The manufacturing of wine grapes is gaining widespread reputation and being carried out on roughly 2,200 hectares of land in Japan. Scions grafted onto rootstocks usually have been imported from the EU, USA, New Zealand, and Australia into Japan. Unfortunately, viruses have unfold in Japanese vineyards by slipping via the web of plant quarantine.
Grapevine rupestris vein feathering virus (GRVFV), which was detected in a Greek grapevine accessions, is a member of genus Marafivirus in household Tymoviridae (El Beaino et al. 2001). GRVFV has been detected in many international locations equivalent to USA, Canada, Australia, New Zealand, Italy, Spain, Switzerland, Czech Republic, Uruguay, and Pakistan (Jo et al. 2015; Eichmeier et al. 2016; Xiao and Meng 2016; Blouin and MacDiarmid 2017; Reynard et al. 2017; Cho et al. 2018; Mahmood et al. 2019; Wu et al. 2020). Herein we report GRVFV an infection in Vitis vinifera L. grapevines from Japan.
In February 2021, dormant canes from 18 V. vinifera cv. Cabernet Sauvignon with leafroll-like illness signs, rising in a winery situated in Kanagawa Prefecture, had been collected. No typical vein banding symptom by GRVFV had been noticed in the grapevines in the course of the rising season.
Total RNA was remoted from the canes utilizing an RNeasy Plant Mini Kit and QIAshredder (Qiagen, Valencia, CA), and subjected to cDNA synthesis utilizing a PrimeScript 1st Strand cDNA Synthesis Kit (Takara Bio, Shiga, Japan). RT-PCR was carried out with GRVFV_6156F and GRVFV_6600R primers for GRVFV detection (Reynard et al. 2017). The anticipated 445 nucleotides (nt) amplification product was obtained from 4 of 18 grapevines.
Sequence evaluation of the merchandise revealed 91% identities to corresponding sequences of GRVFV isolates CHASS (KY513702) and Mauzac (KY513701) from Switzerland. Genome strolling to find out the whole-genome sequence of the GRVFV isolates from the 4 grapevines was carried out. Briefly, the upstream and downstream of the 445 nt amplification product had been amplified from first-strand cDNA utilizing gene-specific primers designed from the product and CHASS-specific primers.
Each amplified fragment was Sanger sequenced. Next, gene-specific primers had been designed to acquire the entire genome of GRVFV as 13 overlapping DNA fragments from every of the 4 grapevine samples. An similar full genome of 6,704 bp was assembled from the overlapping DNA fragments utilizing MEGA 10 software program and named as NA1 isolate (DDBJ accession no. LC619667).
Phylogenetic evaluation of the NA1 genome and corresponding sequences of GRVFV from different international locations confirmed that NA1 fashioned a cluster with isolate NZ ChTK0004 from New Zealand. In pairwise comparisons, the entire NA1 genome was most similar at 88% and 87%, respectively to isolates NZ ChTK0004 and Mauzac.
The predicted amino acid sequences of NA1 polyprotein shared excessive homologies (96%) to the corresponding polyprotein sequences of NZ ChTK0004 and Mauzac, suggesting that NA1 is genetically much like GRVFV isolates from New Zealand and Switzerland.
The NA1-infected Cabernet Sauvignon was co-infected with Grapevine leafroll-associated virus 3, Grapevine virus A, and Grapevine rupestris stem pitting-associated virus in keeping with RT-PCR assay for grapevine virus detection (Nakaune and Nakano 2006). The outcomes underscore the significance of intensifying quarantine measures to stop introduction of unique viruses through contaminated wine grape vegetative cuttings.
Long noncoding RNA uc003pxg.1 regulates endothelial cell proliferation and migration through miR‑25‑5p in coronary artery illness
Long noncoding RNAs (lncRNAs) have been reported to be related to the development of coronary artery illness (CAD). In our earlier examine, the degrees of lncRNA uc003pxg.1 had been upregulated in sufferers with CAD in contrast with these in management topics.
However, the position and underlying mechanism of the results of uc003pxg.1 in CAD stay unknown. Therefore, the goal of the current examine was to research the expression sample and organic operate of uc003pxg.1 in CAD. First, uc003pxg.1 expression ranges had been assessed in peripheral blood mononuclear cells remoted from sufferers with CAD by reverse transcription‑quantitative (RT‑q)PCR.
The outcomes demonstrated that the degrees of uc003pxg.1 had been considerably upregulated (~4.6‑fold) in samples from 80 sufferers with CAD in contrast with these in 80 wholesome topics. Subsequently, the current examine demonstrated that small interfering RNA‑mediated uc003pxg.1 knockdown inhibited human umbilical vein endothelial cell (HUVEC) proliferation and migration, which was analyzed utilizing the Cell Counting Kit‑8, cell cycle, EdU and Transwell assays. Additionally, the outcomes of RT‑qPCR and western blot analyses revealed that uc003pxg.1 regulated the mRNA and protein ranges of cyclin D1 and cyclin‑dependent kinase.
Through excessive‑throughput sequencing and twin‑luciferase reporter assays, the current examine demonstrated that microRNA (miR)‑25‑5p was a downstream goal of uc003pxg.1. Further experiments verified that uc003pxg.1 regulated HUVEC proliferation and migration through miR‑25‑5p. The outcomes of the current examine could improve the present understanding of the position of lncRNA uc003pxg.1 in CAD.
From Sampling to Sequencing: A Liquid Biopsy Pre-Analytic Workflow to Maximize Multi-Layer Genomic Information from a Single Tube
Liquid biopsies maintain nice promise for the administration of most cancers. Reliable liquid biopsy knowledge rely upon secure and reproducible pre-analytical protocols that adjust to high quality measures, irrespective of the sampling and processing website.
We established a workflow for plasma preservation, adopted by processing, cell-free nucleic acid isolation, quantification, and enrichment of probably tumor-derived cell-free DNA and RNA. Employing the identical enter materials for a direct comparability of totally different kits and protocols allowed us to formulate unbiased suggestions for pattern assortment, storage, and processing.
The introduced workflow integrates the stabilization in Norgen, PAX, or Streck tubes and subsequent parallel isolation of cell-free DNA and RNA with NucleoSnap and NucleoSpin. Qubit, Bioanalyzer, and TapeStation quantification and high quality management steps had been optimized for minimal pattern use and excessive sensitivity and reproducibility.
Sodium Bisulfite |
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| 41900224-2 | Bio-WORLD | 50 g | 46.89 EUR |
Sodium Bisulfite |
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| 41900224-3 | Bio-WORLD | 100 g | 75.65 EUR |
Sodium bisulfite |
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| abx182647-5g | Abbexa | 5 g | 226.8 EUR |
Sodium bisulfite |
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| abx182647-96tests | Abbexa | 96 tests | 162.5 EUR |
Sodium bisulfite |
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| GK7777 | Glentham Life Sciences | 100g | 115.8 EUR |
Sodium bisulfite |
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| GK7777-1 | Glentham Life Sciences | 1 | 126.5 EUR |
Sodium bisulfite |
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| GK7777-100 | Glentham Life Sciences | 100 | 23.7 EUR |
Sodium bisulfite |
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| GK7777-1KG | Glentham Life Sciences | 1 kg | 189.6 EUR |
Sodium bisulfite |
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| GK7777-500 | Glentham Life Sciences | 500 | 71.1 EUR |
Sodium bisulfite |
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| GK7777-500G | Glentham Life Sciences | 500 g | 122.4 EUR |
Sodium bisulfite |
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| SB0834 | Bio Basic | 500g | 79.66 EUR |
Sodium bisulfite |
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| S04360 | Pfaltz & Bauer | 500G | 178.4 EUR |
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Description: CAS N° 7631-90-5 |
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Nojirimycin Bisulfite |
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| MBS406003-5mg | MyBiosource | 5mg | 180 EUR |
Nojirimycin Bisulfite |
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| MBS406003-5x5mg | MyBiosource | 5x5mg | 750 EUR |
EpiArt Magnetic DNA Methylation Bisulfite Kit |
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| EM103-00-10rxns | Vazyme | 10 rxns | 1.09 EUR |
EpiArt Magnetic DNA Methylation Bisulfite Kit |
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| EM103-01-50rxns | Vazyme | 50 rxns | 73.58 EUR |
EpiArt Magnetic DNA Methylation Bisulfite Kit |
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| EM103-02-200rxns | Vazyme | 200 rxns | 247.43 EUR |
Glyoxal sodium bisulfite |
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| G04628 | Pfaltz & Bauer | 100G | 148.4 EUR |
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Description: CAS N° 517-21-5 |
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Sodium Bisulfite Solution |
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| SBC125 | ScyTek Laboratories | 125 ml | 11.57 EUR |
Sodium Bisulfite Solution |
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| SBC500 | ScyTek Laboratories | 500 ml | 27 EUR |
Sodium Bisulfite Solution |
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| SBC999 | ScyTek Laboratories | 1000 ml | 51.42 EUR |
Menadione sodium bisulfite |
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| GA0934 | Glentham Life Sciences | 25g | 241.46 EUR |
Menadione sodium bisulfite |
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| GA0934-100 | Glentham Life Sciences | 100 | 263.7 EUR |
Menadione sodium bisulfite |
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| GA0934-25 | Glentham Life Sciences | 25 | 30.1 EUR |
Menadione bisulfite (sodium) |
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| HY-B1897A | MedChemExpress | 10mM/1mL | 135.6 EUR |
Menadione sodium bisulfite |
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| M080-25G | TOKU-E | 25 g | 30.35 EUR |
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Description: 130-37-0 |
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Menadione sodium bisulfite |
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| M080-4x25G | TOKU-E | 4 x 25 g | 80.92 EUR |
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Description: 130-37-0 |
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Menadione sodium bisulfite |
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| MBS5752240-100mg | MyBiosource | 100mg | 220 EUR |
Menadione sodium bisulfite |
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| MBS5752240-10mg | MyBiosource | 10mg | 145 EUR |
Menadione sodium bisulfite |
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| MBS5752240-200mg | MyBiosource | 200mg | 280 EUR |
Menadione sodium bisulfite |
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| MBS5752240-25mg | MyBiosource | 25mg | 155 EUR |
Menadione sodium bisulfite |
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| MBS5752240-50mg | MyBiosource | 50mg | 180 EUR |
Menadione sodium bisulfite |
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| MBS3610516-100mg | MyBiosource | 100mg | 290 EUR |
Menadione sodium bisulfite |
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| MBS3610516-10mg | MyBiosource | 10mg | 200 EUR |
Menadione sodium bisulfite |
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| MBS3610516-200mg | MyBiosource | 200mg | 345 EUR |
Menadione sodium bisulfite |
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| MBS3610516-25mg | MyBiosource | 25mg | 220 EUR |
Menadione sodium bisulfite |
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| MBS3610516-50mg | MyBiosource | 50mg | 245 EUR |
Menadione bisulfite sodium |
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| T5963-10mg | TargetMol Chemicals | 10mg | Ask for price |
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Description: Menadione bisulfite sodium |
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Menadione bisulfite sodium |
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| T5963-1g | TargetMol Chemicals | 1g | Ask for price |
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Description: Menadione bisulfite sodium |
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Menadione bisulfite sodium |
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| T5963-1mg | TargetMol Chemicals | 1mg | Ask for price |
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Description: Menadione bisulfite sodium |
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Menadione bisulfite sodium |
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| T5963-50mg | TargetMol Chemicals | 50mg | Ask for price |
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Description: Menadione bisulfite sodium |
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Menadione bisulfite sodium |
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| T5963-5mg | TargetMol Chemicals | 5mg | Ask for price |
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Description: Menadione bisulfite sodium |
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BisulFlash DNA Bisulfite Conversion Easy Kit |
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| P-1054 | EpiGentek | 50 reactions | 303.6 EUR |
D-Mannojirimycin Bisulfite |
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| M165150 | Toronto Research Chemicals | 10mg | 184 EUR |
Methylamp RNA Bisulfite Conversion Kit |
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| P-9003 | EpiGentek |
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Formaldehyde Sodium Bisulfite |
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| F691460 | Toronto Research Chemicals | 100g | 63 EUR |
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Description: 870-72-4 |
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Galactostatin Bisulfite Adduct |
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| G156500 | Toronto Research Chemicals | 10mg | 227 EUR |
Acetone sodium bisulfite 97% |
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| A04620 | Pfaltz & Bauer | 100G | 270.08 EUR |
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Description: CAS N° 540-92-1 |
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Ammonium Bisulfite - In water |
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| A634143 | Toronto Research Chemicals | 10ml | 69 EUR |
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Description: 10192-30-0 |
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Glutaraldehyde sodium bisulfite |
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| RM9589-250G | EWC Diagnostics | 1 unit | 105.71 EUR |
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Description: Glutaraldehyde sodium bisulfite |
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BisulFlash DNA Bisulfite Conversion Mag-96 Kit |
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| P-1050 | EpiGentek | 192 reactions | 684.66 EUR |
Chloroacetaldehyde Sodium Bisulfite |
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| C382483 | Toronto Research Chemicals | 2.5g | 87 EUR |
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Description: 13064-50-1 |
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T 2588G Sodium Bisulfite adduct |
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| T003103 | Toronto Research Chemicals | 10mg | 1483 EUR |
Adrenochrome Bisulfite Sodium Salt |
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| A305255 | Toronto Research Chemicals | 100mg | 181 EUR |
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Description: 5818-84-8 |
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Menadione Sodium Bisulfite Hydrate |
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| M218663 | Toronto Research Chemicals | 10g | 65 EUR |
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Description: 217487-19-9 |
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Acetone Sodium Bisulfite pure, 98% |
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| 62316 | Sisco Laboratories | 100 Gms | 29.85 EUR |
|
Description: Part B |
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Formaldehyde Sodium Bisulfite- 13CD2 |
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| F702573 | Toronto Research Chemicals | 10mg | 1200 EUR |
Ammonium bisulfite 25% aqueous soln. |
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| A29947 | Pfaltz & Bauer | 250G | 86.8 EUR |
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Description: CAS N° 10192-30-0 |
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Methylamp Whole Cell Bisulfite Modification Kit |
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| P-1016 | EpiGentek |
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Menadione sodium bisulfite hydrate-13C6 |
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| M218105 | Toronto Research Chemicals | 50mg | 15000 EUR |
Glyoxal Sodium Bisulfite (contains oligomers) |
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| G754513 | Toronto Research Chemicals | 10g | 58 EUR |
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Description: 517-21-5 |
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Formaldehyde/sodium bisulfite addition compound |
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| F05023 | Pfaltz & Bauer | 500G | 74 EUR |
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Description: CAS N° 870-72-4 |
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Ammonium bisulfite 45% w/v aqueous solution |
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| A29946 | Pfaltz & Bauer | 250G | 168.8 EUR |
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Description: CAS N° 10192-30-0 |
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Glutaraldehyde sodium bisulfite addition compound |
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| G02580 | Pfaltz & Bauer | 50G | 177.16 EUR |
|
Description: CAS N° 7420-89-5 |
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3-Indoleacetaldehyde bisulfite addition compound |
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| I01290 | Pfaltz & Bauer | 25MG | 239.38 EUR |
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Description: CAS N° 20095-27-6 |
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Sodium Bisulfite (mixture of NaHSO3 and Na2S2O5) |
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| S614400 | Toronto Research Chemicals | 100g | 80 EUR |
|
Description: 7631-90-5 |
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pOET Sequencing Primers |
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| GWB-200100 | GenWay Biotech | 2 x 100 ul | Ask for price |
Sequencing Reaction Clean-Up Kit |
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| 34500 | Norgen Biotek Corp | 50 Preps | 77.4 EUR |
SequaGel Sequencing System 1L Kit - 1KIT |
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| NAT1136 | Scientific Laboratory Supplies | 1KIT | 187.65 EUR |
SEQUENCING UNIT 33X45 CM |
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| ESEQ1100-SYS | Consort | ea | 2060 EUR |
Galactostatin Bisulfite (5-Amino-5-deoxy-D-galactopyranose, Galactonojirimycin Bisulfite, 6-(Hydroxymethyl)-2,3,4,6-peperidinetetrol Hydrogensulfite) |
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| MBS6043222-1mg | MyBiosource | 1(mg | 590 EUR |
Galactostatin Bisulfite (5-Amino-5-deoxy-D-galactopyranose, Galactonojirimycin Bisulfite, 6-(Hydroxymethyl)-2,3,4,6-peperidinetetrol Hydrogensulfite) |
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| MBS6043222-5x1mg | MyBiosource | 5x1mg | 2500 EUR |
SequaGel Sequencing System 2.2L Kit - EACH |
|||
| NAT1138 | Scientific Laboratory Supplies | EACH | 318.6 EUR |
Palmitaldehyde/Sodium Bisulfite Addition Compound _x000D__x000D_ |
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| P00520 | Pfaltz & Bauer | 5G | 171.23 EUR |
|
Description: CAS N° 7355-88-6 |
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Sequencing Grade Chymotrypsin |
|||
| RE013 | SAB | 100ug | 309 EUR |
SEQUENCING SYSTEM 20X50 CM |
|||
| ESEQ1200-SYS | Consort | ea | 2060 EUR |
Trypin for Mass & Sequencing |
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| T9600-025 | GenDepot | 25ug | 76 EUR |
Trypin for Mass & Sequencing |
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| T9600-100 | GenDepot | 100ug | 133 EUR |
Trypin for Mass & Sequencing |
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| T9600-112 | GenDepot | 12x100ug | 1230 EUR |
Trypin for Mass & Sequencing |
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| T9600-400 | GenDepot | 4x100ug | 470 EUR |
5,6-Diamino-2,4-dihydroxypyrimidine-13C2 Bisulfite Salt |
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| D416392 | Toronto Research Chemicals | 10mg | 161 EUR |
Sequencing Grade V8 Protease |
|||
| RE014 | SAB | 50ug | 209 EUR |
5-Hydroxyindole-3-acetaldehyde Sodium Bisulfite Adduct |
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| H942933 | Toronto Research Chemicals | 500mg | 3000 EUR |
TNM Sequencing Primers for SE |
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| TMS002-C1-1rxn | Vazyme | 1 rxn | 115 EUR |
TNM Sequencing Primers for PE |
|||
| TMS002-C2-1rxn | Vazyme | 1 rxn | 143.88 EUR |
Chymotrypsin for Sequencing grade |
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| C4001-010 | GenDepot | 4x25ug | 375.6 EUR |
Chymotrypsin for Sequencing grade |
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| C4001-100 | GenDepot | 100ug | 343.2 EUR |
Sequencing Grade Modified Trypsin |
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| RE012 | SAB | 100ug | 229 EUR |
EpiNext Post-Bisulfite DNA Library Preparation Kit (Illumina) |
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| P-1055 | EpiGentek |
|
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Oligo, Sequencing Primer, T3 (17mer) |
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| MBS634243-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, T3 (17mer) |
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| MBS634243-5x0033mg | MyBiosource | 5x0.033mg | 1235 EUR |
Oligo, Sequencing Primer, T7 (17mer) |
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| MBS634247-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, T7 (17mer) |
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| MBS634247-5x0033mg | MyBiosource | 5x0.033mg | 1235 EUR |
Oligo, Sequencing Primer, KS (19mer) |
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| MBS634259-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, KS (19mer) |
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| MBS634259-5x0033mg | MyBiosource | 5x0.033mg | 1235 EUR |
Oligo, Sequencing Primer, T3 (20mer) |
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| MBS634261-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, T3 (20mer) |
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| MBS634261-5x0033mg | MyBiosource | 5x0.033mg | 1240 EUR |
Oligo, Sequencing Primer, SK (17mer) |
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| MBS634188-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, SK (17mer) |
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| MBS634188-5x0033mg | MyBiosource | 5x0.033mg | 1235 EUR |
Oligo, Sequencing Primer, KS (17mer) |
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| MBS634192-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, KS (17mer) |
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| MBS634192-5x0033mg | MyBiosource | 5x0.033mg | 1235 EUR |
Oligo, Sequencing Primer, SK (20mer) |
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| MBS634219-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, SK (20mer) |
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| MBS634219-5x0033mg | MyBiosource | 5x0.033mg | 1240 EUR |
10 X TTE DNA Sequencing Grade |
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| IBS-BT028 | iNtRON Biotechnology Inc | 500 mL | 35 EUR |
Sequencing Reaction Clean-Up 96-Well Kit |
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| 34400 | Norgen Biotek Corp | 2 Plates | 155.4 EUR |
DNA Library Prep Kit for IIlumina Sequencing |
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| K1475-12 | Biovision | 12 Rxns | 576 EUR |
Sequencing Grade Carboxypeptidase B |
|||
| RE011 | SAB | 100ug | 99 EUR |
PCR Clean Up for DNA Sequencing |
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| BT5100 | Bio Basic | 100preps | 114.82 EUR |
PCR Clean Up for DNA Sequencing |
|||
| BT5101 | Bio Basic | 1000Preps, 1000prep | 553.3 EUR |
ACES Sequencing Buffer 10X, pH 7.7 |
|||
| 21420000-1 | Bio-WORLD | 1 L | 172.16 EUR |
|
Description: 2- (N-morpholino) ethanesulfonic acid) buffer |
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ACES Sequencing Buffer 10X, pH 7.7 |
|||
| 21420000-2 | Bio-WORLD | 4 L | 539 EUR |
|
Description: 2- (N-morpholino) ethanesulfonic acid) buffer |
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TBE-Buffer 5X for DNA Sequencing |
|||
| 40121072-1 | Bio-WORLD | 100 mL | 32.55 EUR |
TBE-Buffer 5X for DNA Sequencing |
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| 40121072-2 | Bio-WORLD | 500 mL | 54.99 EUR |
TBE-Buffer 5X for DNA Sequencing |
|||
| 40121072-3 | Bio-WORLD | 1 L | 89.78 EUR |
TBE-Buffer 5X for DNA Sequencing |
|||
| 40121072-4 | Bio-WORLD | 4 L | 235.65 EUR |
Bovine Trypsin Purified Sequencing Grade |
|||
| IBOTRYSEQ100UG | Innovative research | each | 148 EUR |
|
Description: Bovine Trypsin Purified Sequencing Grade |
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Bovine Trypsin Purified Sequencing Grade |
|||
| IBOTRYSEQ1MG | Innovative research | each | 765 EUR |
|
Description: Bovine Trypsin Purified Sequencing Grade |
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Bovine Trypsin Purified Sequencing Grade |
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| IBOTRYSEQ400UG | Innovative research | each | 419 EUR |
|
Description: Bovine Trypsin Purified Sequencing Grade |
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Bovine Trypsin Purified Sequencing Grade |
|||
| MBS136298-01mg | MyBiosource | 0.1mg | 255 EUR |
Bovine Trypsin Purified Sequencing Grade |
|||
| MBS136298-04mg | MyBiosource | 0.4mg | 540 EUR |
Bovine Trypsin Purified Sequencing Grade |
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| MBS136298-1mg | MyBiosource | 1mg | 935 EUR |
Bovine Trypsin Purified Sequencing Grade |
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| MBS136298-5x1mg | MyBiosource | 5x1mg | 4010 EUR |
MagaDye™ 4 Color Sanger Sequencing Terminator Kit |
|||
| 17068 | AAT Bioquest | 5 nmoles | 2091 EUR |
Oligo, Sequencing Primer, SP6 Promoter (19mer) |
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| MBS634260-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, SP6 Promoter (19mer) |
|||
| MBS634260-5x0033mg | MyBiosource | 5x0.033mg | 1235 EUR |
Oligo, Sequencing Primer, T7 Universal (20mer) |
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| MBS634194-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, T7 Universal (20mer) |
|||
| MBS634194-5x0033mg | MyBiosource | 5x0.033mg | 1240 EUR |
Trifluoroacetic Acid (TFA) for HPLC & Sequencing, 99.9% |
|||
| 23947 | Sisco Laboratories | 10 x1 ml | 165.03 EUR |
|
Description: Part A |
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Oligo, Sequencing Primer, Lambda gt11, Forward (24mer) |
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| MBS634252-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, Lambda gt11, Forward (24mer) |
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| MBS634252-5x0033mg | MyBiosource | 5x0.033mg | 1245 EUR |
Oligo, Sequencing Primer, Lambda gt10, Forward (27mer) |
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| MBS634255-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, Lambda gt10, Forward (27mer) |
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| MBS634255-5x0033mg | MyBiosource | 5x0.033mg | 1240 EUR |
Oligo, Sequencing Primer, Lambda gt11, Reverse (24mer) |
|||
| MBS634120-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, Lambda gt11, Reverse (24mer) |
|||
| MBS634120-5x0033mg | MyBiosource | 5x0.033mg | 1245 EUR |
Oligo, Sequencing Primer, Lambda gt10, Reverse (31mer) |
|||
| MBS634167-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, Lambda gt10, Reverse (31mer) |
|||
| MBS634167-5x0033mg | MyBiosource | 5x0.033mg | 1245 EUR |
D-Mannojirimycin Bisulfite (5-Amino-5-deoxy-D-mannopyranose Hydrogensulfite) |
|||
| MBS6055354-1mg | MyBiosource | 1(mg | 580 EUR |
D-Mannojirimycin Bisulfite (5-Amino-5-deoxy-D-mannopyranose Hydrogensulfite) |
|||
| MBS6055354-5x1mg | MyBiosource | 5x1mg | 2470 EUR |
Bovine Trypsin Purified Sequencing Grade Lyophilized |
|||
| IBOTRYSEQLY100UG | Innovative research | each | 130 EUR |
|
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized |
|||
Bovine Trypsin Purified Sequencing Grade Lyophilized |
|||
| IBOTRYSEQLY1MG | Innovative research | each | 640 EUR |
|
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized |
|||
Bovine Trypsin Purified Sequencing Grade Lyophilized |
|||
| IBOTRYSEQLY400UG | Innovative research | each | 344 EUR |
|
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized |
|||
Bovine Trypsin Purified Sequencing Grade Lyophilized |
|||
| MBS136299-01mg | MyBiosource | 0.1mg | 235 EUR |
Bovine Trypsin Purified Sequencing Grade Lyophilized |
|||
| MBS136299-04mg | MyBiosource | 0.4mg | 465 EUR |
Bovine Trypsin Purified Sequencing Grade Lyophilized |
|||
| MBS136299-1mg | MyBiosource | 1mg | 795 EUR |
Bovine Trypsin Purified Sequencing Grade Lyophilized |
|||
| MBS136299-5x1mg | MyBiosource | 5x1mg | 3400 EUR |
PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System) |
|||
| 60200 | Norgen Biotek Corp | 50 Preps | 83.4 EUR |
Oligo, Sequencing Primer, pBR322 (BamH I), Clockwise (20mer) |
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| MBS634234-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, pBR322 (BamH I), Clockwise (20mer) |
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| MBS634234-5x0033mg | MyBiosource | 5x0.033mg | 1240 EUR |
Oligo, Sequencing Primer, pBR322 (EcoR I), Clockwise (16mer) |
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| MBS634158-0033mg | MyBiosource | 0.033mg | 305 EUR |
Oligo, Sequencing Primer, pBR322 (EcoR I), Clockwise (16mer) |
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| MBS634158-5x0033mg | MyBiosource | 5x0.033mg | 1230 EUR |
Oligo, Sequencing Primer, pBR322 (Pst I), Counterclockwise (16mer) |
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| MBS634143-0033mg | MyBiosource | 0.033mg | 305 EUR |
Oligo, Sequencing Primer, pBR322 (Pst I), Counterclockwise (16mer) |
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| MBS634143-5x0033mg | MyBiosource | 5x0.033mg | 1230 EUR |
Oligo, Sequencing Primer, pBR322 (BamH I), Counterclockwise (16mer) |
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| MBS634262-0033mg | MyBiosource | 0.033mg | 305 EUR |
Oligo, Sequencing Primer, pBR322 (BamH I), Counterclockwise (16mer) |
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| MBS634262-5x0033mg | MyBiosource | 5x0.033mg | 1230 EUR |
PCR and Sequencing Reaction Clean-Up 96-Well Kit (Magnetic Bead System) |
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| 62700 | Norgen Biotek Corp | 2 Plates | 227.4 EUR |
Oligo, Sequencing Primer, pBR322 (Hind III), Counterclockwise (16mer) |
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| MBS634154-0033mg | MyBiosource | 0.033mg | 305 EUR |
Oligo, Sequencing Primer, pBR322 (Hind III), Counterclockwise (16mer) |
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| MBS634154-5x0033mg | MyBiosource | 5x0.033mg | 1230 EUR |
EpiNext CUT&RUN Fast Kit |
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| P-2028 | EpiGentek | 24 Reactions | 340 EUR |
EpiNext CUT&RUN Fast Kit |
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| P-2028-24 | EpiGentek | 24 reactions | 359.7 EUR |
Genorise® Customized PCR primers for cloning and sequencing |
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| GR108023 | Genorise Scientific | 2 nmol | 65 EUR |
EpiNext CUT&Tag In-Place (cTIP) Seq Kit |
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| P-2032 | EpiGentek |
|
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EpiNext RRBS Library Fast Kit |
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| P-1069 | EpiGentek |
|
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EpiNext DNA Size Selection Kit |
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| P-1059 | EpiGentek |
|
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Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade |
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| IBOCHYTLCKSG100UG | Innovative research | each | 297 EUR |
|
Description: Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade |
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Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade |
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| MBS136223-01mg | MyBiosource | 0.1mg | 400 EUR |
Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade |
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| MBS136223-5x01mg | MyBiosource | 5x0.1mg | 1605 EUR |
Oligo, Sequencing Primer, M13 Reverse (16mer) [5'-AAC AGC TAT GAC CAT G-3'] |
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| MBS634131-0033mg | MyBiosource | 0.033mg | 305 EUR |
Oligo, Sequencing Primer, M13 Reverse (16mer) [5'-AAC AGC TAT GAC CAT G-3'] |
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| MBS634131-5x0033mg | MyBiosource | 5x0.033mg | 1230 EUR |
Oligo, Sequencing Primer, M13 (-20) Forward (17mer) [5'-GTA AAA CGA CGG CCA GT-3'] |
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| MBS634146-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, M13 (-20) Forward (17mer) [5'-GTA AAA CGA CGG CCA GT-3'] |
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| MBS634146-5x0033mg | MyBiosource | 5x0.033mg | 1235 EUR |
Oligo, Sequencing Primer, M13 Reverse (19mer) [5'-GGA AAC AGC TAT GAC CAT G-3'] |
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| MBS634148-0033mg | MyBiosource | 0.033mg | 310 EUR |
Oligo, Sequencing Primer, M13 Reverse (19mer) [5'-GGA AAC AGC TAT GAC CAT G-3'] |
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| MBS634148-5x0033mg | MyBiosource | 5x0.033mg | 1235 EUR |
EpiNext Hi-Fi cDNA Synthesis Kit |
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| P-9004 | EpiGentek |
|
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EpiNext CUT&RUN RNA m6A-Seq Kit |
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| P-9016 | EpiGentek |
|
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Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified |
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| IBCTCLOSGPF10UG | Innovative research | each | 165 EUR |
|
Description: Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified |
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Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified |
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| MBS136171-01mg | MyBiosource | 0.1mg | 270 EUR |
Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified |
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| MBS136171-5x001mg | MyBiosource | 5x0.01mg | 1035 EUR |
Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized |
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| IBCTV8SEQLY250UG | Innovative research | each | 784 EUR |
|
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized |
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Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized |
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| IBCTV8SEQLY50UG | Innovative research | each | 304 EUR |
|
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized |
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EpiNext DNA Library Preparation Kit (Illumina) |
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| P-1051 | EpiGentek |
|
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EpiNext DNA Purification HT System |
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| P-1063 | EpiGentek |
|
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EpiNext NGS Barcode (Index) Set-12 |
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| P-1060 | EpiGentek |
|
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EpiNext ChIP-Seq High-Sensitivity Kit (Illumina) |
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| P-2030 | EpiGentek |
|
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SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK |
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| AM-96-SEPTA-3100 | CORNING | 10/pk | 639.6 EUR |
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Description: Sealing Products; Sealing mats - Axygen |
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EpiNext High-Sensitivity DNA Library Preparation Kit (Illumina) |
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| P-1053 | EpiGentek |
|
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BisulFlash DNA Modification Kit |
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| P-1026 | EpiGentek |
|
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Sinapine bisulfate |
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| TBZ1907 | ChemNorm | unit | Ask for price |
Potassium bisulfate 97% |
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| P22531 | Pfaltz & Bauer | 100G | 140.16 EUR |
|
Description: CAS N° 7646-93-7 |
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(±)-Clopidogrel (bisulfate) |
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| HY-107867 | MedChemExpress | 10 mg | 54.11 EUR |
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Description: Clopidogrel sulfate is an antiplatelet agent. Specifically, Clopidogrel sulfate inhibits the binding of ADP to its receptors on the membranes of platelet cells, and blocks ADP-mediated activation of the glycoprotein GPIIb/IIIa complex. Clopidogrel sulfate can be used for research of heart disease and stroke[1]. |
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Atazanavir Bisulfate Salt |
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| A790050 | Toronto Research Chemicals | 10mg | 184 EUR |
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Description: 229975-97-7 |
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Atazanavir Bisulfate Salt |
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| MBS6054340-25mg | MyBiosource | 2.5(mg | 390 EUR |
Atazanavir Bisulfate Salt |
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| MBS6054340-5x25mg | MyBiosource | 5x2.5mg | 1600 EUR |
Ammonium bisulfate 98%_x000D__x000D_ |
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| A29945 | Pfaltz & Bauer | 500G | 244.4 EUR |
|
Description: CAS N° 7803-63-6 |
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Sodium bisulfate, monohydrate |
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| SB7834 | Bio Basic | 500g | 73.57 EUR |
rac-Clopidogrel-D3 Bisulfate |
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| ESS0319-10mg | ESSchemco | 10 mg | 192.5 EUR |
Tetrapropylammonium Bisulfate |
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| T310590 | Toronto Research Chemicals | 100mg | 178 EUR |
|
Description: 56211-70-2 |
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Coproporphyrin III Sodium BIsulfate Salt |
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| C685415 | Toronto Research Chemicals | 50mg | 7600 EUR |
Coproporphyrin III-15N4 Sodium BIsulfate Salt |
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| C685417 | Toronto Research Chemicals | 0.25mg | 5474 EUR |
ACES™ Signal Sequence Kit |
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| 0148-2 | AthenaES | kit | 264 EUR |
Pyridin-3-ylacetaldehyde Bisulfate Adduct (~90%) |
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| P991758 | Toronto Research Chemicals | 100mg | 184 EUR |
We present the effectivity of the proposed workflow by profitable droplet digital PCR amplification of each cell-free DNA and RNA and by detection of tumor-specific alterations in low-coverage whole-genome sequencing and DNA methylation profiling of plasma-derived cell-free DNA. For the primary time, we demonstrated profitable parallel extraction of cell-free DNA and RNA from plasma samples. This workflow paves the highway in the direction of multi-layer genomic evaluation from one single liquid biopsy pattern.