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First report of grapevine rupestris vein feathering virus in Vitis vinifera L. from Japan

First report of grapevine rupestris vein feathering virus in Vitis vinifera L. from Japan

Posted on September 11, 2021July 23, 2021 by Vasil

The manufacturing of wine grapes is gaining widespread reputation and being carried out on roughly 2,200 hectares of land in Japan. Scions grafted onto rootstocks usually have been imported from the EU, USA, New Zealand, and Australia into Japan. Unfortunately, viruses have unfold in Japanese vineyards by slipping via the web of plant quarantine.

Grapevine rupestris vein feathering virus (GRVFV), which was detected in a Greek grapevine accessions, is a member of genus Marafivirus in household Tymoviridae (El Beaino et al. 2001). GRVFV has been detected in many international locations equivalent to USA, Canada, Australia, New Zealand, Italy, Spain, Switzerland, Czech Republic, Uruguay, and Pakistan (Jo et al. 2015; Eichmeier et al. 2016; Xiao and Meng 2016; Blouin and MacDiarmid 2017; Reynard et al. 2017; Cho et al. 2018; Mahmood et al. 2019; Wu et al. 2020). Herein we report GRVFV an infection in Vitis vinifera L. grapevines from Japan.

In February 2021, dormant canes from 18 V. vinifera cv. Cabernet Sauvignon with leafroll-like illness signs, rising in a winery situated in Kanagawa Prefecture, had been collected. No typical vein banding symptom by GRVFV had been noticed in the grapevines in the course of the rising season.

Total RNA was remoted from the canes utilizing an RNeasy Plant Mini Kit and QIAshredder (Qiagen, Valencia, CA), and subjected to cDNA synthesis utilizing a PrimeScript 1st Strand cDNA Synthesis Kit (Takara Bio, Shiga, Japan). RT-PCR was carried out with GRVFV_6156F and GRVFV_6600R primers for GRVFV detection (Reynard et al. 2017). The anticipated 445 nucleotides (nt) amplification product was obtained from 4 of 18 grapevines.

Sequence evaluation of the merchandise revealed 91% identities to corresponding sequences of GRVFV isolates CHASS (KY513702) and Mauzac (KY513701) from Switzerland. Genome strolling to find out the whole-genome sequence of the GRVFV isolates from the 4 grapevines was carried out. Briefly, the upstream and downstream of the 445 nt amplification product had been amplified from first-strand cDNA utilizing gene-specific primers designed from the product and CHASS-specific primers.

Each amplified fragment was Sanger sequenced. Next, gene-specific primers had been designed to acquire the entire genome of GRVFV as 13 overlapping DNA fragments from every of the 4 grapevine samples. An similar full genome of 6,704 bp was assembled from the overlapping DNA fragments utilizing MEGA 10 software program and named as NA1 isolate (DDBJ accession no. LC619667).

Phylogenetic evaluation of the NA1 genome and corresponding sequences of GRVFV from different international locations confirmed that NA1 fashioned a cluster with isolate NZ ChTK0004 from New Zealand. In pairwise comparisons, the entire NA1 genome was most similar at 88% and 87%, respectively to isolates NZ ChTK0004 and Mauzac.

The predicted amino acid sequences of NA1 polyprotein shared excessive homologies (96%) to the corresponding polyprotein sequences of NZ ChTK0004 and Mauzac, suggesting that NA1 is genetically much like GRVFV isolates from New Zealand and Switzerland.

First report of grapevine rupestris vein feathering virus in Vitis vinifera L. from Japan

The NA1-infected Cabernet Sauvignon was co-infected with Grapevine leafroll-associated virus 3, Grapevine virus A, and Grapevine rupestris stem pitting-associated virus in keeping with RT-PCR assay for grapevine virus detection (Nakaune and Nakano 2006). The outcomes underscore the significance of intensifying quarantine measures to stop introduction of unique viruses through contaminated wine grape vegetative cuttings.

Long noncoding RNA uc003pxg.1 regulates endothelial cell proliferation and migration through miR‑25‑5p in coronary artery illness

Long noncoding RNAs (lncRNAs) have been reported to be related to the development of coronary artery illness (CAD). In our earlier examine, the degrees of lncRNA uc003pxg.1 had been upregulated in sufferers with CAD in contrast with these in management topics.

However, the position and underlying mechanism of the results of uc003pxg.1 in CAD stay unknown. Therefore, the goal of the current examine was to research the expression sample and organic operate of uc003pxg.1 in CAD. First, uc003pxg.1 expression ranges had been assessed in peripheral blood mononuclear cells remoted from sufferers with CAD by reverse transcription‑quantitative (RT‑q)PCR.

The outcomes demonstrated that the degrees of uc003pxg.1 had been considerably upregulated (~4.6‑fold) in samples from 80 sufferers with CAD in contrast with these in 80 wholesome topics. Subsequently, the current examine demonstrated that small interfering RNA‑mediated uc003pxg.1 knockdown inhibited human umbilical vein endothelial cell (HUVEC) proliferation and migration, which was analyzed utilizing the Cell Counting Kit‑8, cell cycle, EdU and Transwell assays. Additionally, the outcomes of RT‑qPCR and western blot analyses revealed that uc003pxg.1 regulated the mRNA and protein ranges of cyclin D1 and cyclin‑dependent kinase.

Through excessive‑throughput sequencing and twin‑luciferase reporter assays, the current examine demonstrated that microRNA (miR)‑25‑5p was a downstream goal of uc003pxg.1. Further experiments verified that uc003pxg.1 regulated HUVEC proliferation and migration through miR‑25‑5p. The outcomes of the current examine could improve the present understanding of the position of lncRNA uc003pxg.1 in CAD.

From Sampling to Sequencing: A Liquid Biopsy Pre-Analytic Workflow to Maximize Multi-Layer Genomic Information from a Single Tube

Liquid biopsies maintain nice promise for the administration of most cancers. Reliable liquid biopsy knowledge rely upon secure and reproducible pre-analytical protocols that adjust to high quality measures, irrespective of the sampling and processing website.

We established a workflow for plasma preservation, adopted by processing, cell-free nucleic acid isolation, quantification, and enrichment of probably tumor-derived cell-free DNA and RNA. Employing the identical enter materials for a direct comparability of totally different kits and protocols allowed us to formulate unbiased suggestions for pattern assortment, storage, and processing.

The introduced workflow integrates the stabilization in Norgen, PAX, or Streck tubes and subsequent parallel isolation of cell-free DNA and RNA with NucleoSnap and NucleoSpin. Qubit, Bioanalyzer, and TapeStation quantification and high quality management steps had been optimized for minimal pattern use and excessive sensitivity and reproducibility.

Sodium Bisulfite

41900224-2 Bio-WORLD 50 g 46.89 EUR

Sodium Bisulfite

41900224-3 Bio-WORLD 100 g 75.65 EUR

Sodium bisulfite

abx182647-5g Abbexa 5 g 226.8 EUR

Sodium bisulfite

abx182647-96tests Abbexa 96 tests 162.5 EUR

Sodium bisulfite

GK7777 Glentham Life Sciences 100g 115.8 EUR

Sodium bisulfite

GK7777-1 Glentham Life Sciences 1 126.5 EUR

Sodium bisulfite

GK7777-100 Glentham Life Sciences 100 23.7 EUR

Sodium bisulfite

GK7777-1KG Glentham Life Sciences 1 kg 189.6 EUR

Sodium bisulfite

GK7777-500 Glentham Life Sciences 500 71.1 EUR

Sodium bisulfite

GK7777-500G Glentham Life Sciences 500 g 122.4 EUR

Sodium bisulfite

SB0834 Bio Basic 500g 79.66 EUR

Sodium bisulfite

S04360 Pfaltz & Bauer 500G 178.4 EUR
Description: CAS N° 7631-90-5

Nojirimycin Bisulfite

MBS406003-5mg MyBiosource 5mg 180 EUR

Nojirimycin Bisulfite

MBS406003-5x5mg MyBiosource 5x5mg 750 EUR

EpiArt Magnetic DNA Methylation Bisulfite Kit

EM103-00-10rxns Vazyme 10 rxns 1.09 EUR

EpiArt Magnetic DNA Methylation Bisulfite Kit

EM103-01-50rxns Vazyme 50 rxns 73.58 EUR

EpiArt Magnetic DNA Methylation Bisulfite Kit

EM103-02-200rxns Vazyme 200 rxns 247.43 EUR

Glyoxal sodium bisulfite

G04628 Pfaltz & Bauer 100G 148.4 EUR
Description: CAS N° 517-21-5

Sodium Bisulfite Solution

SBC125 ScyTek Laboratories 125 ml 11.57 EUR

Sodium Bisulfite Solution

SBC500 ScyTek Laboratories 500 ml 27 EUR

Sodium Bisulfite Solution

SBC999 ScyTek Laboratories 1000 ml 51.42 EUR

Menadione sodium bisulfite

GA0934 Glentham Life Sciences 25g 241.46 EUR

Menadione sodium bisulfite

GA0934-100 Glentham Life Sciences 100 263.7 EUR

Menadione sodium bisulfite

GA0934-25 Glentham Life Sciences 25 30.1 EUR

Menadione bisulfite (sodium)

HY-B1897A MedChemExpress 10mM/1mL 135.6 EUR

Menadione sodium bisulfite

M080-25G TOKU-E 25 g 30.35 EUR
Description: 130-37-0

Menadione sodium bisulfite

M080-4x25G TOKU-E 4 x 25 g 80.92 EUR
Description: 130-37-0

Menadione sodium bisulfite

MBS5752240-100mg MyBiosource 100mg 220 EUR

Menadione sodium bisulfite

MBS5752240-10mg MyBiosource 10mg 145 EUR

Menadione sodium bisulfite

MBS5752240-200mg MyBiosource 200mg 280 EUR

Menadione sodium bisulfite

MBS5752240-25mg MyBiosource 25mg 155 EUR

Menadione sodium bisulfite

MBS5752240-50mg MyBiosource 50mg 180 EUR

Menadione sodium bisulfite

MBS3610516-100mg MyBiosource 100mg 290 EUR

Menadione sodium bisulfite

MBS3610516-10mg MyBiosource 10mg 200 EUR

Menadione sodium bisulfite

MBS3610516-200mg MyBiosource 200mg 345 EUR

Menadione sodium bisulfite

MBS3610516-25mg MyBiosource 25mg 220 EUR

Menadione sodium bisulfite

MBS3610516-50mg MyBiosource 50mg 245 EUR

Menadione bisulfite sodium

T5963-10mg TargetMol Chemicals 10mg Ask for price
Description: Menadione bisulfite sodium

Menadione bisulfite sodium

T5963-1g TargetMol Chemicals 1g Ask for price
Description: Menadione bisulfite sodium

Menadione bisulfite sodium

T5963-1mg TargetMol Chemicals 1mg Ask for price
Description: Menadione bisulfite sodium

Menadione bisulfite sodium

T5963-50mg TargetMol Chemicals 50mg Ask for price
Description: Menadione bisulfite sodium

Menadione bisulfite sodium

T5963-5mg TargetMol Chemicals 5mg Ask for price
Description: Menadione bisulfite sodium

BisulFlash DNA Bisulfite Conversion Easy Kit

P-1054 EpiGentek 50 reactions 303.6 EUR

D-Mannojirimycin Bisulfite

M165150 Toronto Research Chemicals 10mg 184 EUR

Methylamp RNA Bisulfite Conversion Kit  

P-9003 EpiGentek
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  • 50 Reactions
  • 50 Reactions

Formaldehyde Sodium Bisulfite

F691460 Toronto Research Chemicals 100g 63 EUR
Description: 870-72-4

Galactostatin Bisulfite Adduct

G156500 Toronto Research Chemicals 10mg 227 EUR

Acetone sodium bisulfite 97%

A04620 Pfaltz & Bauer 100G 270.08 EUR
Description: CAS N° 540-92-1

Ammonium Bisulfite - In water

A634143 Toronto Research Chemicals 10ml 69 EUR
Description: 10192-30-0

Glutaraldehyde sodium bisulfite

RM9589-250G EWC Diagnostics 1 unit 105.71 EUR
Description: Glutaraldehyde sodium bisulfite

BisulFlash DNA Bisulfite Conversion Mag-96 Kit

P-1050 EpiGentek 192 reactions 684.66 EUR

Chloroacetaldehyde Sodium Bisulfite

C382483 Toronto Research Chemicals 2.5g 87 EUR
Description: 13064-50-1

T 2588G Sodium Bisulfite adduct

T003103 Toronto Research Chemicals 10mg 1483 EUR

Adrenochrome Bisulfite Sodium Salt

A305255 Toronto Research Chemicals 100mg 181 EUR
Description: 5818-84-8

Menadione Sodium Bisulfite Hydrate

M218663 Toronto Research Chemicals 10g 65 EUR
Description: 217487-19-9

Acetone Sodium Bisulfite pure, 98%

62316 Sisco Laboratories 100 Gms 29.85 EUR
Description: Part B

Formaldehyde Sodium Bisulfite- 13CD2

F702573 Toronto Research Chemicals 10mg 1200 EUR

Ammonium bisulfite 25% aqueous soln.

A29947 Pfaltz & Bauer 250G 86.8 EUR
Description: CAS N° 10192-30-0

Methylamp Whole Cell Bisulfite Modification Kit 

P-1016 EpiGentek
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  • 80 Samples
  • 40 Samples
  • 80 Samples

Menadione sodium bisulfite hydrate-13C6

M218105 Toronto Research Chemicals 50mg 15000 EUR

Glyoxal Sodium Bisulfite (contains oligomers)

G754513 Toronto Research Chemicals 10g 58 EUR
Description: 517-21-5

Formaldehyde/sodium bisulfite addition compound

F05023 Pfaltz & Bauer 500G 74 EUR
Description: CAS N° 870-72-4

Ammonium bisulfite 45% w/v aqueous solution

A29946 Pfaltz & Bauer 250G 168.8 EUR
Description: CAS N° 10192-30-0

Glutaraldehyde sodium bisulfite addition compound

G02580 Pfaltz & Bauer 50G 177.16 EUR
Description: CAS N° 7420-89-5

3-Indoleacetaldehyde bisulfite addition compound

I01290 Pfaltz & Bauer 25MG 239.38 EUR
Description: CAS N° 20095-27-6

Sodium Bisulfite (mixture of NaHSO3 and Na2S2O5)

S614400 Toronto Research Chemicals 100g 80 EUR
Description: 7631-90-5

pOET Sequencing Primers

GWB-200100 GenWay Biotech 2 x 100 ul Ask for price

Sequencing Reaction Clean-Up Kit

34500 Norgen Biotek Corp 50 Preps 77.4 EUR

SequaGel Sequencing System 1L Kit - 1KIT

NAT1136 Scientific Laboratory Supplies 1KIT 187.65 EUR

SEQUENCING UNIT 33X45 CM

ESEQ1100-SYS Consort ea 2060 EUR

Galactostatin Bisulfite (5-Amino-5-deoxy-D-galactopyranose, Galactonojirimycin Bisulfite, 6-(Hydroxymethyl)-2,3,4,6-peperidinetetrol Hydrogensulfite)

MBS6043222-1mg MyBiosource 1(mg 590 EUR

Galactostatin Bisulfite (5-Amino-5-deoxy-D-galactopyranose, Galactonojirimycin Bisulfite, 6-(Hydroxymethyl)-2,3,4,6-peperidinetetrol Hydrogensulfite)

MBS6043222-5x1mg MyBiosource 5x1mg 2500 EUR

SequaGel Sequencing System 2.2L Kit - EACH

NAT1138 Scientific Laboratory Supplies EACH 318.6 EUR

Palmitaldehyde/Sodium Bisulfite Addition Compound _x000D__x000D_

P00520 Pfaltz & Bauer 5G 171.23 EUR
Description: CAS N° 7355-88-6

Sequencing Grade Chymotrypsin

RE013 SAB 100ug 309 EUR

SEQUENCING SYSTEM 20X50 CM

ESEQ1200-SYS Consort ea 2060 EUR

Trypin for Mass & Sequencing

T9600-025 GenDepot 25ug 76 EUR

Trypin for Mass & Sequencing

T9600-100 GenDepot 100ug 133 EUR

Trypin for Mass & Sequencing

T9600-112 GenDepot 12x100ug 1230 EUR

Trypin for Mass & Sequencing

T9600-400 GenDepot 4x100ug 470 EUR

5,6-Diamino-2,4-dihydroxypyrimidine-13C2 Bisulfite Salt

D416392 Toronto Research Chemicals 10mg 161 EUR

Sequencing Grade V8 Protease

RE014 SAB 50ug 209 EUR

5-Hydroxyindole-3-acetaldehyde Sodium Bisulfite Adduct

H942933 Toronto Research Chemicals 500mg 3000 EUR

TNM Sequencing Primers for SE

TMS002-C1-1rxn Vazyme 1 rxn 115 EUR

TNM Sequencing Primers for PE

TMS002-C2-1rxn Vazyme 1 rxn 143.88 EUR

Chymotrypsin for Sequencing grade

C4001-010 GenDepot 4x25ug 375.6 EUR

Chymotrypsin for Sequencing grade

C4001-100 GenDepot 100ug 343.2 EUR

Sequencing Grade Modified Trypsin

RE012 SAB 100ug 229 EUR

EpiNext Post-Bisulfite DNA Library Preparation Kit (Illumina)  

P-1055 EpiGentek
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  • 24 Reactions
  • 12 Reactions
  • 24 Reactions

Oligo, Sequencing Primer, T3 (17mer)

MBS634243-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, T3 (17mer)

MBS634243-5x0033mg MyBiosource 5x0.033mg 1235 EUR

Oligo, Sequencing Primer, T7 (17mer)

MBS634247-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, T7 (17mer)

MBS634247-5x0033mg MyBiosource 5x0.033mg 1235 EUR

Oligo, Sequencing Primer, KS (19mer)

MBS634259-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, KS (19mer)

MBS634259-5x0033mg MyBiosource 5x0.033mg 1235 EUR

Oligo, Sequencing Primer, T3 (20mer)

MBS634261-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, T3 (20mer)

MBS634261-5x0033mg MyBiosource 5x0.033mg 1240 EUR

Oligo, Sequencing Primer, SK (17mer)

MBS634188-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, SK (17mer)

MBS634188-5x0033mg MyBiosource 5x0.033mg 1235 EUR

Oligo, Sequencing Primer, KS (17mer)

MBS634192-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, KS (17mer)

MBS634192-5x0033mg MyBiosource 5x0.033mg 1235 EUR

Oligo, Sequencing Primer, SK (20mer)

MBS634219-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, SK (20mer)

MBS634219-5x0033mg MyBiosource 5x0.033mg 1240 EUR

10 X TTE DNA Sequencing Grade

IBS-BT028 iNtRON Biotechnology Inc 500 mL 35 EUR

Sequencing Reaction Clean-Up 96-Well Kit

34400 Norgen Biotek Corp 2 Plates 155.4 EUR

DNA Library Prep Kit for IIlumina Sequencing

K1475-12 Biovision 12 Rxns 576 EUR

Sequencing Grade Carboxypeptidase B

RE011 SAB 100ug 99 EUR

PCR Clean Up for DNA Sequencing

BT5100 Bio Basic 100preps 114.82 EUR

PCR Clean Up for DNA Sequencing

BT5101 Bio Basic 1000Preps, 1000prep 553.3 EUR

ACES Sequencing Buffer 10X, pH 7.7

21420000-1 Bio-WORLD 1 L 172.16 EUR
Description: 2- (N-morpholino) ethanesulfonic acid) buffer

ACES Sequencing Buffer 10X, pH 7.7

21420000-2 Bio-WORLD 4 L 539 EUR
Description: 2- (N-morpholino) ethanesulfonic acid) buffer

TBE-Buffer 5X for DNA Sequencing

40121072-1 Bio-WORLD 100 mL 32.55 EUR

TBE-Buffer 5X for DNA Sequencing

40121072-2 Bio-WORLD 500 mL 54.99 EUR

TBE-Buffer 5X for DNA Sequencing

40121072-3 Bio-WORLD 1 L 89.78 EUR

TBE-Buffer 5X for DNA Sequencing

40121072-4 Bio-WORLD 4 L 235.65 EUR

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ100UG Innovative research each 148 EUR
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ1MG Innovative research each 765 EUR
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

IBOTRYSEQ400UG Innovative research each 419 EUR
Description: Bovine Trypsin Purified Sequencing Grade

Bovine Trypsin Purified Sequencing Grade

MBS136298-01mg MyBiosource 0.1mg 255 EUR

Bovine Trypsin Purified Sequencing Grade

MBS136298-04mg MyBiosource 0.4mg 540 EUR

Bovine Trypsin Purified Sequencing Grade

MBS136298-1mg MyBiosource 1mg 935 EUR

Bovine Trypsin Purified Sequencing Grade

MBS136298-5x1mg MyBiosource 5x1mg 4010 EUR

MagaDye™ 4 Color Sanger Sequencing Terminator Kit

17068 AAT Bioquest 5 nmoles 2091 EUR

Oligo, Sequencing Primer, SP6 Promoter (19mer)

MBS634260-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, SP6 Promoter (19mer)

MBS634260-5x0033mg MyBiosource 5x0.033mg 1235 EUR

Oligo, Sequencing Primer, T7 Universal (20mer)

MBS634194-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, T7 Universal (20mer)

MBS634194-5x0033mg MyBiosource 5x0.033mg 1240 EUR

Trifluoroacetic Acid (TFA) for HPLC & Sequencing, 99.9%

23947 Sisco Laboratories 10 x1 ml 165.03 EUR
Description: Part A

Oligo, Sequencing Primer, Lambda gt11, Forward (24mer)

MBS634252-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, Lambda gt11, Forward (24mer)

MBS634252-5x0033mg MyBiosource 5x0.033mg 1245 EUR

Oligo, Sequencing Primer, Lambda gt10, Forward (27mer)

MBS634255-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, Lambda gt10, Forward (27mer)

MBS634255-5x0033mg MyBiosource 5x0.033mg 1240 EUR

Oligo, Sequencing Primer, Lambda gt11, Reverse (24mer)

MBS634120-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, Lambda gt11, Reverse (24mer)

MBS634120-5x0033mg MyBiosource 5x0.033mg 1245 EUR

Oligo, Sequencing Primer, Lambda gt10, Reverse (31mer)

MBS634167-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, Lambda gt10, Reverse (31mer)

MBS634167-5x0033mg MyBiosource 5x0.033mg 1245 EUR

D-Mannojirimycin Bisulfite (5-Amino-5-deoxy-D-mannopyranose Hydrogensulfite)

MBS6055354-1mg MyBiosource 1(mg 580 EUR

D-Mannojirimycin Bisulfite (5-Amino-5-deoxy-D-mannopyranose Hydrogensulfite)

MBS6055354-5x1mg MyBiosource 5x1mg 2470 EUR

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY100UG Innovative research each 130 EUR
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY1MG Innovative research each 640 EUR
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

IBOTRYSEQLY400UG Innovative research each 344 EUR
Description: Bovine Trypsin Purified Sequencing Grade Lyophilized

Bovine Trypsin Purified Sequencing Grade Lyophilized

MBS136299-01mg MyBiosource 0.1mg 235 EUR

Bovine Trypsin Purified Sequencing Grade Lyophilized

MBS136299-04mg MyBiosource 0.4mg 465 EUR

Bovine Trypsin Purified Sequencing Grade Lyophilized

MBS136299-1mg MyBiosource 1mg 795 EUR

Bovine Trypsin Purified Sequencing Grade Lyophilized

MBS136299-5x1mg MyBiosource 5x1mg 3400 EUR

PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System)

60200 Norgen Biotek Corp 50 Preps 83.4 EUR

Oligo, Sequencing Primer, pBR322 (BamH I), Clockwise (20mer)

MBS634234-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, pBR322 (BamH I), Clockwise (20mer)

MBS634234-5x0033mg MyBiosource 5x0.033mg 1240 EUR

Oligo, Sequencing Primer, pBR322 (EcoR I), Clockwise (16mer)

MBS634158-0033mg MyBiosource 0.033mg 305 EUR

Oligo, Sequencing Primer, pBR322 (EcoR I), Clockwise (16mer)

MBS634158-5x0033mg MyBiosource 5x0.033mg 1230 EUR

Oligo, Sequencing Primer, pBR322 (Pst I), Counterclockwise (16mer)

MBS634143-0033mg MyBiosource 0.033mg 305 EUR

Oligo, Sequencing Primer, pBR322 (Pst I), Counterclockwise (16mer)

MBS634143-5x0033mg MyBiosource 5x0.033mg 1230 EUR

Oligo, Sequencing Primer, pBR322 (BamH I), Counterclockwise (16mer)

MBS634262-0033mg MyBiosource 0.033mg 305 EUR

Oligo, Sequencing Primer, pBR322 (BamH I), Counterclockwise (16mer)

MBS634262-5x0033mg MyBiosource 5x0.033mg 1230 EUR

PCR and Sequencing Reaction Clean-Up 96-Well Kit (Magnetic Bead System)

62700 Norgen Biotek Corp 2 Plates 227.4 EUR

Oligo, Sequencing Primer, pBR322 (Hind III), Counterclockwise (16mer)

MBS634154-0033mg MyBiosource 0.033mg 305 EUR

Oligo, Sequencing Primer, pBR322 (Hind III), Counterclockwise (16mer)

MBS634154-5x0033mg MyBiosource 5x0.033mg 1230 EUR

EpiNext CUT&RUN Fast Kit

P-2028 EpiGentek 24 Reactions 340 EUR

EpiNext CUT&RUN Fast Kit

P-2028-24 EpiGentek 24 reactions 359.7 EUR

Genorise® Customized PCR primers for cloning and sequencing

GR108023 Genorise Scientific 2 nmol 65 EUR

EpiNext CUT&Tag In-Place (cTIP) Seq Kit

P-2032 EpiGentek
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  • 12 reactions
  • 24 reactions

EpiNext RRBS Library Fast Kit

P-1069 EpiGentek
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  • 12 Reactions
  • 24 Reactions

EpiNext DNA Size Selection Kit

P-1059 EpiGentek
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  • 96 reactions
  • 48 reactions
  • 96 reactions

Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

IBOCHYTLCKSG100UG Innovative research each 297 EUR
Description: Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

MBS136223-01mg MyBiosource 0.1mg 400 EUR

Bovine Alpha Chymotrypsin 3X Crystallized TLCK Treated Sequencing Grade

MBS136223-5x01mg MyBiosource 5x0.1mg 1605 EUR

Oligo, Sequencing Primer, M13 Reverse (16mer) [5'-AAC AGC TAT GAC CAT G-3']

MBS634131-0033mg MyBiosource 0.033mg 305 EUR

Oligo, Sequencing Primer, M13 Reverse (16mer) [5'-AAC AGC TAT GAC CAT G-3']

MBS634131-5x0033mg MyBiosource 5x0.033mg 1230 EUR

Oligo, Sequencing Primer, M13 (-20) Forward (17mer) [5'-GTA AAA CGA CGG CCA GT-3']

MBS634146-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, M13 (-20) Forward (17mer) [5'-GTA AAA CGA CGG CCA GT-3']

MBS634146-5x0033mg MyBiosource 5x0.033mg 1235 EUR

Oligo, Sequencing Primer, M13 Reverse (19mer) [5'-GGA AAC AGC TAT GAC CAT G-3']

MBS634148-0033mg MyBiosource 0.033mg 310 EUR

Oligo, Sequencing Primer, M13 Reverse (19mer) [5'-GGA AAC AGC TAT GAC CAT G-3']

MBS634148-5x0033mg MyBiosource 5x0.033mg 1235 EUR

EpiNext Hi-Fi cDNA Synthesis Kit  

P-9004 EpiGentek
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  • 20 Samples
  • 20 Reactions

EpiNext CUT&RUN RNA m6A-Seq Kit

P-9016 EpiGentek
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  • 12 reactions
  • 24 reactions

Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

IBCTCLOSGPF10UG Innovative research each 165 EUR
Description: Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

Bacteria-Derived Clostripain (Endoproteinase-Arg-C) Sequencing Grade Purified

MBS136171-01mg MyBiosource 0.1mg 270 EUR

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Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

IBCTV8SEQLY250UG Innovative research each 784 EUR
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

IBCTV8SEQLY50UG Innovative research each 304 EUR
Description: Bacteria-Derived V8 Protease (Endoprotease Glu-C) Sequencing Grade Lyophilized

EpiNext DNA Library Preparation Kit (Illumina)  

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EpiNext DNA Purification HT System

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EpiNext NGS Barcode (Index) Set-12  

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SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK

AM-96-SEPTA-3100 CORNING 10/pk 639.6 EUR
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BisulFlash DNA Modification Kit 

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Sinapine bisulfate

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Potassium bisulfate 97%

P22531 Pfaltz & Bauer 100G 140.16 EUR
Description: CAS N° 7646-93-7

(±)-Clopidogrel (bisulfate)

HY-107867 MedChemExpress 10 mg 54.11 EUR
Description: Clopidogrel sulfate is an antiplatelet agent. Specifically, Clopidogrel sulfate inhibits the binding of ADP to its receptors on the membranes of platelet cells, and blocks ADP-mediated activation of the glycoprotein GPIIb/IIIa complex. Clopidogrel sulfate can be used for research of heart disease and stroke[1].

Atazanavir Bisulfate Salt

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Atazanavir Bisulfate Salt

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Atazanavir Bisulfate Salt

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Ammonium bisulfate 98%_x000D__x000D_

A29945 Pfaltz & Bauer 500G 244.4 EUR
Description: CAS N° 7803-63-6

Sodium bisulfate, monohydrate

SB7834 Bio Basic 500g 73.57 EUR

rac-Clopidogrel-D3 Bisulfate

ESS0319-10mg ESSchemco 10 mg 192.5 EUR

Tetrapropylammonium Bisulfate

T310590 Toronto Research Chemicals 100mg 178 EUR
Description: 56211-70-2

Coproporphyrin III Sodium BIsulfate Salt

C685415 Toronto Research Chemicals 50mg 7600 EUR

Coproporphyrin III-15N4 Sodium BIsulfate Salt

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ACES™ Signal Sequence Kit

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Pyridin-3-ylacetaldehyde Bisulfate Adduct (~90%)

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We present the effectivity of the proposed workflow by profitable droplet digital PCR amplification of each cell-free DNA and RNA and by detection of tumor-specific alterations in low-coverage whole-genome sequencing and DNA methylation profiling of plasma-derived cell-free DNA. For the primary time, we demonstrated profitable parallel extraction of cell-free DNA and RNA from plasma samples. This workflow paves the highway in the direction of multi-layer genomic evaluation from one single liquid biopsy pattern.

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  • Mouse SALa(Salusin Alpha) ELISA Kit
  • Mouse SALb(Salusin Beta) ELISA Kit
  • Mouse SEMA4D(Semaphorin 4D) ELISA Kit
  • Mouse SEPP1(Selenoprotein P1, Plasma) ELISA Kit
  • Mouse SPB(Surfactant Associated Protein B) ELISA Kit
  • Mouse SPC(Surfactant Associated Protein C) ELISA Kit
  • Mouse SYP(Synaptophysin) ELISA Kit
  • Mouse TFF1(Trefoil Factor 1) ELISA Kit
  • Mouse TLN(Talin) ELISA Kit
  • Mouse TLR9(Toll Like Receptor 9) ELISA Kit
  • Mouse UCN2(Urocortin 2) ELISA Kit
  • Mouse VEGF121(Vascular Endothelial Growth Factor 121) ELISA Kit
  • Mouse VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit
  • Mouse VF(Visfatin) ELISA Kit
  • Pig ACE(Angiotensin I Converting Enzyme) ELISA Kit
  • Pig ADP(Adiponectin) ELISA Kit
  • Pig aHSG(Alpha-2-Heremans Schmid Glycoprotein) ELISA Kit
  • Pig ANGPT2(Angiopoietin 2) ELISA Kit
  • Pig APOA2(Apolipoprotein A2) ELISA Kit
  • Pig C3(Complement Component 3) ELISA Kit
  • Pig CCK(Cholecystokinin) ELISA Kit
  • Pig COL3(Collagen Type III) ELISA Kit
  • Pig CRP(C Reactive Protein) ELISA Kit
  • Pig CST3(Cystatin 3) ELISA Kit
  • Pig D2D(D-Dimer) ELISA Kit
  • Pig DAO(Diamine Oxidase) ELISA Kit
  • Pig EGF(Epidermal Growth Factor) ELISA Kit
  • Pig FGF7(Fibroblast Growth Factor 7) ELISA Kit
  • Pig GH(Growth Hormone) ELISA Kit
  • Pig GHRH(Growth Hormone Releasing Hormone) ELISA Kit
  • Pig HIF1a(Hypoxia Inducible Factor 1 Alpha) ELISA Kit
  • Pig Hpt(Haptoglobin) ELISA Kit
  • Pig IFNa(Interferon Alpha) ELISA Kit
  • Pig IFNb(Interferon Beta) ELISA Kit
  • Pig IgE(Immunoglobulin E) ELISA Kit
  • Pig IGF1(Insulin Like Growth Factor 1) ELISA Kit
  • Pig IGF2(Insulin Like Growth Factor 2) ELISA Kit
  • Pig IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit
  • Pig IL12B(Interleukin 12B) ELISA Kit
  • Pig IL13(Interleukin 13) ELISA Kit
  • Pig IL17(Interleukin 17) ELISA Kit
  • Pig IL18(Interleukin 18) ELISA Kit
  • Pig IL1a(Interleukin 1 Alpha) ELISA Kit
  • Pig IL2(Interleukin 2) ELISA Kit
  • Pig IL4(Interleukin 4) ELISA Kit
  • Pig IL5(Interleukin 5) ELISA Kit
  • Pig LEP(Leptin) ELISA Kit
  • Pig MMP9(Matrix Metalloproteinase 9) ELISA Kit
  • Pig MPO(Myeloperoxidase) ELISA Kit
  • Pig NOS3(Nitric Oxide Synthase 3, Endothelial) ELISA Kit
  • Pig OC(Osteocalcin) ELISA Kit
  • Pig SELE(Selectin, Endothelium) ELISA Kit
  • Pig SELP(Selectin, Platelet) ELISA Kit
  • Pig SPA(Surfactant Associated Protein A) ELISA Kit
  • Pig SST(Somatostatin) ELISA Kit
  • Pig TF(Tissue Factor) ELISA Kit
  • Pig TGFb1(Transforming Growth Factor Beta 1) ELISA Kit
  • Pig TIMP1(Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit
  • Pig TM(Thrombomodulin) ELISA Kit
  • Pig TNNI3(Troponin I Type 3, Cardiac) ELISA Kit
  • Pig tPA(Plasminogen Activator, Tissue) ELISA Kit
  • Pig VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit
  • Pig vWF(Von Willebrand Factor) ELISA Kit
  • Rabbit APOA1(Apolipoprotein A1) ELISA Kit
  • Rabbit FDP(Fibrinogen Degradation Product) ELISA Kit
  • Rabbit FSH(Follicle Stimulating Hormone) ELISA Kit
  • Rabbit Hpt(Haptoglobin) ELISA Kit
  • Rabbit IFNb(Interferon Beta) ELISA Kit
  • Rabbit LOX1(Lectin Like Oxidized Low Density Lipoprotein Receptor 1) ELISA Kit
  • Rabbit MMP10(Matrix Metalloproteinase 10) ELISA Kit
  • Rabbit NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
  • Rabbit S100A11(S100 Calcium Binding Protein A11) ELISA Kit
  • Rabbit SAA(Serum Amyloid A) ELISA Kit
  • Rabbit TLR9(Toll Like Receptor 9) ELISA Kit
  • Rat a1M(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
  • Rat ABCG2(ATP Binding Cassette Transporter G2) ELISA Kit
  • Rat ACE2(Angiotensin I Converting Enzyme 2) ELISA Kit
  • Rat ACVB(Activin B) ELISA Kit
  • Rat ADAM9(A Disintegrin And Metalloprotease 9) ELISA Kit
  • Rat ADM(Adrenomedullin) ELISA Kit
  • Rat AIF(Apoptosis Inducing Factor) ELISA Kit
  • Rat ALOX5(Arachidonate-5-Lipoxygenase) ELISA Kit
  • Rat ALP(Alkaline Phosphatase) ELISA Kit
  • Rat ALPL(Alkaline Phosphatase, Liver/Bone/Kidney) ELISA Kit
  • Rat AngIII(Angiotensin III) ELISA Kit
  • Rat ANP(Atrial Natriuretic Peptide) ELISA Kit
  • Rat ANXA2(Annexin A2) ELISA Kit
  • Rat APOB(Apolipoprotein B) ELISA Kit
  • Rat APOC1(Apolipoprotein C1) ELISA Kit
  • Rat AST(Aspartate Aminotransferase) ELISA Kit
  • Rat Bax(Bcl2 Associated X Protein) ELISA Kit
  • Rat BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
  • Rat BNP(Brain Natriuretic Peptide) ELISA Kit
  • Rat C1INH(Complement 1 Inhibitor) ELISA Kit
  • Rat C1q(Complement 1q) ELISA Kit
  • Rat C3(Complement Component 3) ELISA Kit
  • Rat CA2(Carbonic Anhydrase II) ELISA Kit
  • Rat CACT(Carnitine Acylcarnitine Translocase) ELISA Kit
  • Rat CAMP(Cathelicidin Antimicrobial Peptide) ELISA Kit
  • Rat CaN(Calcineurin) ELISA Kit
  • Rat CAPNS1(Calpain, Small Subunit 1) ELISA Kit
  • Rat CCK8(Cholecystokinin 8, Octapeptide) ELISA Kit
  • Rat ChAT(Choline Acetyltransferase) ELISA Kit
  • Rat CHGA(Chromogranin A) ELISA Kit
  • Rat CLU(Clusterin) ELISA Kit
  • Rat COMP(Cartilage Oligomeric Matrix Protein) ELISA Kit
  • Rat CPP(Copeptin) ELISA Kit
  • Rat CSTA(Cystatin A) ELISA Kit
  • Rat CSTB(Cystatin B) ELISA Kit
  • Rat CYSLTR2(Cysteinyl Leukotriene Receptor 2) ELISA Kit
  • Rat DAT(Dopamine Transporter) ELISA Kit
  • Rat DEFb1(Defensin Beta 1) ELISA Kit
  • Rat Dyn(Big Dynorphin) ELISA Kit
  • Rat EMILIN1(Elastin Microfibril Interface Located Protein 1) ELISA Kit
  • Rat ERa(Estrogen Receptor Alpha) ELISA Kit
  • Rat FABP3(Fatty Acid Binding Protein 3, Muscle And Heart) ELISA Kit
  • Rat FETUB(Fetuin B) ELISA Kit
  • Rat FGa(Fibrinogen Alpha) ELISA Kit
  • Rat FGF10(Fibroblast Growth Factor 10) ELISA Kit
  • Rat FGF2(Fibroblast Growth Factor 2, Basic) ELISA Kit
  • Rat FGF23(Fibroblast Growth Factor 23) ELISA Kit
  • Rat FOS(V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog) ELISA Kit
  • Rat FS(Follistatin) ELISA Kit
  • Rat GAL(Galanin) ELISA Kit
  • Rat GAL4(Galectin 4) ELISA Kit
  • Rat GDF15(Growth Differentiation Factor 15) ELISA Kit
  • Rat GnRH(Gonadotropin Releasing Hormone) ELISA Kit
  • Rat GS(Glutamine synthetase) ELISA Kit
  • Rat HAT1(Histone Acetyltransferase 1) ELISA Kit
  • Rat HbA1c(Glycated Hemoglobin A1c) ELISA Kit
  • Rat HDL(High Density Lipoprotein) ELISA Kit
  • Rat HPX(Hemopexin) ELISA Kit
  • Rat IDO(Indoleamine-2,3-Dioxygenase) ELISA Kit
  • Rat IL17B(Interleukin 17B) ELISA Kit
  • Rat IL21(Interleukin 21) ELISA Kit
  • Rat IL2Ra(Interleukin 2 Receptor Alpha) ELISA Kit
  • Rat IL6R(Interleukin 6 Receptor) ELISA Kit
  • Rat IL7R(Interleukin 7 Receptor) ELISA Kit
  • Rat IL8Rb(Interleukin 8 Receptor Beta) ELISA Kit
  • Rat INHA(Inhibin A) ELISA Kit
  • Rat INHB(Inhibin B) ELISA Kit
  • Rat ITGaM(Integrin Alpha M) ELISA Kit
  • Rat LBP(Lipopolysaccharide Binding Protein) ELISA Kit
  • Rat LDH(Lactate Dehydrogenase) ELISA Kit
  • Rat LOX1(Lectin Like Oxidized Low Density Lipoprotein Receptor 1) ELISA Kit
  • Rat LRG1(Leucine Rich Alpha-2-Glycoprotein 1) ELISA Kit
  • Rat MAP1A(Microtubule Associated Protein 1A) ELISA Kit
  • Rat MCP1(Monocyte Chemotactic Protein 1) ELISA Kit
  • Rat MEP1a(Meprin A Alpha) ELISA Kit
  • Rat MT1(Metallothionein 1) ELISA Kit
  • Rat MUC5B(Mucin 5 Subtype B) ELISA Kit
  • Rat NFkB(Nuclear Factor Kappa B) ELISA Kit
  • Rat NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
  • Rat NOX4(Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4) ELISA Kit
  • Rat NR3C1(Nuclear Receptor Subfamily 3, Group C, Member 1) ELISA Kit
  • Rat NRG1(Neuregulin 1) ELISA Kit
  • Rat Ntn1(Netrin 1) ELISA Kit
  • Rat PDGFB(Platelet Derived Growth Factor Subunit B) ELISA Kit
  • Rat PIICP(Procollagen II C-Terminal Propeptide) ELISA Kit
  • Rat PIIINP(Procollagen III N-Terminal Propeptide) ELISA Kit
  • Rat PKCa(Protein Kinase C Alpha) ELISA Kit
  • Rat PKCz(Protein Kinase C Zeta) ELISA Kit
  • Rat Plg(Plasminogen) ELISA Kit
  • Rat PP(Pancreatic Polypeptide) ELISA Kit
  • Rat PR3(Proteinase 3) ELISA Kit
  • Rat PRKAb1(Protein Kinase, AMP Activated Beta 1) ELISA Kit
  • Rat PTHrP(Parathyroid Hormone Related Protein) ELISA Kit
  • Rat SDH(Sorbitol Dehydrogenase) ELISA Kit
  • Rat SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit
  • Rat SPA2(Surfactant Associated Protein A2) ELISA Kit
  • Rat SPD(Surfactant Associated Protein D) ELISA Kit
  • Rat SYP(Synaptophysin) ELISA Kit
  • Rat TARC(Thymus Activation Regulated Chemokine) ELISA Kit
  • Rat TFF3(Trefoil Factor 3, Intestinal) ELISA Kit
  • Rat TGFbR1(Transforming Growth Factor Beta Receptor I) ELISA Kit
  • Rat TNC(Tenascin C) ELISA Kit
  • Rat TrxR1(Thioredoxin Reductase 1) ELISA Kit
  • Rat UCN1(Urocortin 1) ELISA Kit
  • Rat VEGFR2(Vascular Endothelial Growth Factor Receptor 2) ELISA Kit
  • Rat vWF(Von Willebrand Factor) ELISA Kit
  • Sheep TIMP1(Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit
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