The manufacturing of wine grapes is gaining widespread reputation and being carried out on roughly 2,200 hectares of land in Japan. Scions grafted onto rootstocks usually have been imported from the EU, USA, New Zealand, and Australia into Japan. Unfortunately, viruses have unfold in Japanese vineyards by slipping via the web of plant quarantine.
Grapevine rupestris vein feathering virus (GRVFV), which was detected in a Greek grapevine accessions, is a member of genus Marafivirus in household Tymoviridae (El Beaino et al. 2001). GRVFV has been detected in many international locations equivalent to USA, Canada, Australia, New Zealand, Italy, Spain, Switzerland, Czech Republic, Uruguay, and Pakistan (Jo et al. 2015; Eichmeier et al. 2016; Xiao and Meng 2016; Blouin and MacDiarmid 2017; Reynard et al. 2017; Cho et al. 2018; Mahmood et al. 2019; Wu et al. 2020). Herein we report GRVFV an infection in Vitis vinifera L. grapevines from Japan.
In February 2021, dormant canes from 18 V. vinifera cv. Cabernet Sauvignon with leafroll-like illness signs, rising in a winery situated in Kanagawa Prefecture, had been collected. No typical vein banding symptom by GRVFV had been noticed in the grapevines in the course of the rising season.
Total RNA was remoted from the canes utilizing an RNeasy Plant Mini Kit and QIAshredder (Qiagen, Valencia, CA), and subjected to cDNA synthesis utilizing a PrimeScript 1st Strand cDNA Synthesis Kit (Takara Bio, Shiga, Japan). RT-PCR was carried out with GRVFV_6156F and GRVFV_6600R primers for GRVFV detection (Reynard et al. 2017). The anticipated 445 nucleotides (nt) amplification product was obtained from 4 of 18 grapevines.
Sequence evaluation of the merchandise revealed 91% identities to corresponding sequences of GRVFV isolates CHASS (KY513702) and Mauzac (KY513701) from Switzerland. Genome strolling to find out the whole-genome sequence of the GRVFV isolates from the 4 grapevines was carried out. Briefly, the upstream and downstream of the 445 nt amplification product had been amplified from first-strand cDNA utilizing gene-specific primers designed from the product and CHASS-specific primers.
Each amplified fragment was Sanger sequenced. Next, gene-specific primers had been designed to acquire the entire genome of GRVFV as 13 overlapping DNA fragments from every of the 4 grapevine samples. An similar full genome of 6,704 bp was assembled from the overlapping DNA fragments utilizing MEGA 10 software program and named as NA1 isolate (DDBJ accession no. LC619667).
Phylogenetic evaluation of the NA1 genome and corresponding sequences of GRVFV from different international locations confirmed that NA1 fashioned a cluster with isolate NZ ChTK0004 from New Zealand. In pairwise comparisons, the entire NA1 genome was most similar at 88% and 87%, respectively to isolates NZ ChTK0004 and Mauzac.
The predicted amino acid sequences of NA1 polyprotein shared excessive homologies (96%) to the corresponding polyprotein sequences of NZ ChTK0004 and Mauzac, suggesting that NA1 is genetically much like GRVFV isolates from New Zealand and Switzerland.
The NA1-infected Cabernet Sauvignon was co-infected with Grapevine leafroll-associated virus 3, Grapevine virus A, and Grapevine rupestris stem pitting-associated virus in keeping with RT-PCR assay for grapevine virus detection (Nakaune and Nakano 2006). The outcomes underscore the significance of intensifying quarantine measures to stop introduction of unique viruses through contaminated wine grape vegetative cuttings.
Long noncoding RNA uc003pxg.1 regulates endothelial cell proliferation and migration through miR‑25‑5p in coronary artery illness
Long noncoding RNAs (lncRNAs) have been reported to be related to the development of coronary artery illness (CAD). In our earlier examine, the degrees of lncRNA uc003pxg.1 had been upregulated in sufferers with CAD in contrast with these in management topics.
However, the position and underlying mechanism of the results of uc003pxg.1 in CAD stay unknown. Therefore, the goal of the current examine was to research the expression sample and organic operate of uc003pxg.1 in CAD. First, uc003pxg.1 expression ranges had been assessed in peripheral blood mononuclear cells remoted from sufferers with CAD by reverse transcription‑quantitative (RT‑q)PCR.
The outcomes demonstrated that the degrees of uc003pxg.1 had been considerably upregulated (~4.6‑fold) in samples from 80 sufferers with CAD in contrast with these in 80 wholesome topics. Subsequently, the current examine demonstrated that small interfering RNA‑mediated uc003pxg.1 knockdown inhibited human umbilical vein endothelial cell (HUVEC) proliferation and migration, which was analyzed utilizing the Cell Counting Kit‑8, cell cycle, EdU and Transwell assays. Additionally, the outcomes of RT‑qPCR and western blot analyses revealed that uc003pxg.1 regulated the mRNA and protein ranges of cyclin D1 and cyclin‑dependent kinase.
Through excessive‑throughput sequencing and twin‑luciferase reporter assays, the current examine demonstrated that microRNA (miR)‑25‑5p was a downstream goal of uc003pxg.1. Further experiments verified that uc003pxg.1 regulated HUVEC proliferation and migration through miR‑25‑5p. The outcomes of the current examine could improve the present understanding of the position of lncRNA uc003pxg.1 in CAD.
From Sampling to Sequencing: A Liquid Biopsy Pre-Analytic Workflow to Maximize Multi-Layer Genomic Information from a Single Tube
Liquid biopsies maintain nice promise for the administration of most cancers. Reliable liquid biopsy knowledge rely upon secure and reproducible pre-analytical protocols that adjust to high quality measures, irrespective of the sampling and processing website.
We established a workflow for plasma preservation, adopted by processing, cell-free nucleic acid isolation, quantification, and enrichment of probably tumor-derived cell-free DNA and RNA. Employing the identical enter materials for a direct comparability of totally different kits and protocols allowed us to formulate unbiased suggestions for pattern assortment, storage, and processing.
The introduced workflow integrates the stabilization in Norgen, PAX, or Streck tubes and subsequent parallel isolation of cell-free DNA and RNA with NucleoSnap and NucleoSpin. Qubit, Bioanalyzer, and TapeStation quantification and high quality management steps had been optimized for minimal pattern use and excessive sensitivity and reproducibility.
 Sodium bisulfite |
|
abx182647-5g |
Abbexa |
5 g |
EUR 189 |
- Shipped within 1-2 weeks.
|
Sodium bisulfite |
|
SB0834 |
Bio Basic |
500g |
EUR 66.38 |
- Product category: Biochemicals/Misc. Biochemicals
|
 EpiNext DNA Size Selection Kit |
|
P-1059 |
EpiGentek |
96 reactions |
EUR 300.85 |
|
Description: The best epigenetics products |
) Menadione bisulfite (sodium) |
|
HY-B1897A |
MedChemExpress |
10mM/1mL |
EUR 113 |
 Menadione sodium bisulfite |
|
M080-25G |
TOKU-E |
25 g |
EUR 64 |
Menadione sodium bisulfite |
|
M080-4x25G |
TOKU-E |
4 x 25 g |
EUR 121 |
 EpiArt DNA Methylation Bisulfite Kit |
|
EM101-01 |
Vazyme |
50 rxn |
EUR 241 |
EpiArt DNA Methylation Bisulfite Kit |
|
EM101-02 |
Vazyme |
200 rxn |
EUR 609 |
 Methylamp RNA Bisulfite Conversion Kit |
|
P-9003 |
EpiGentek |
50 Reactions |
EUR 300.85 |
|
Description: kits suitable for this type of research |
 Chymotrypsin for Sequencing grade |
|
C4001-010 |
GenDepot |
4x25ug |
EUR 313 |
Chymotrypsin for Sequencing grade |
|
C4001-100 |
GenDepot |
100ug |
EUR 286 |
 sequencing unit 33x45 cm |
|
ESEQ1100-SYS |
Consort |
ea |
EUR 1535 |
 sequencing system 20x50 cm |
|
ESEQ1200-SYS |
Consort |
ea |
EUR 1535 |
 Trypin for Mass & Sequencing |
|
T9600-025 |
GenDepot |
25ug |
EUR 145 |
Trypin for Mass & Sequencing |
|
T9600-100 |
GenDepot |
100ug |
EUR 219 |
Trypin for Mass & Sequencing |
|
T9600-112 |
GenDepot |
12x100ug |
EUR 1657 |
Trypin for Mass & Sequencing |
|
T9600-400 |
GenDepot |
4x100ug |
EUR 651 |
 EpiNext Hi-Fi cDNA Synthesis Kit |
|
P-9004 |
EpiGentek |
20 Samples |
EUR 318.25 |
|
Description: Ask the seller for details |
) EpiNext DNA Library Preparation Kit (Illumina) |
|
P-1051 |
EpiGentek |
24 Reactions |
EUR 1179.55 |
|
Description: kits suitable for this type of research |
 EpiNext DNA Purification HT System |
|
P-1063 |
EpiGentek |
480 ml |
EUR 6022.55 |
|
Description: The best epigenetics products |
 Methylamp Whole Cell Bisulfite Modification Kit |
|
P-1016 |
EpiGentek |
80 Samples |
EUR 496.6 |
|
Description: The best epigenetics products |
 BisulFlash DNA Bisulfite Conversion Easy Kit |
|
P-1054 |
EpiGentek |
50 reactions |
EUR 253 |
|
Description: kits suitable for this type of research |
) EpiNext ChIP-Seq High-Sensitivity Kit (Illumina) |
|
P-2030 |
EpiGentek |
24 Reactions |
EUR 1498.55 |
|
Description: The best epigenetics products |
 DNA Library Prep Kit for IIlumina Sequencing |
|
K1475-12 |
Biovision |
12 Rxns |
EUR 480 |
 Set-12) EpiNext NGS Barcode (Index) Set-12 |
|
P-1060 |
EpiGentek |
144 Reactions |
EUR 197.9 |
|
Description: reagents widely cited |
 BisulFlash DNA Bisulfite Conversion Mag-96 Kit |
|
P-1050 |
EpiGentek |
192 reactions |
EUR 570.55 |
|
Description: The best epigenetics products |
) EpiNext High-Sensitivity DNA Library Preparation Kit (Illumina) |
|
P-1053 |
EpiGentek |
24 Reactions |
EUR 1179.55 |
|
Description: reagents widely cited |
 PCR Clean Up for DNA Sequencing |
|
BT5100 |
Bio Basic |
100preps |
EUR 95.68 |
- Product category: PCR Related/PCR Kits (Cleanup)
|
PCR Clean Up for DNA Sequencing |
|
BT5101 |
Bio Basic |
1000Preps, 1000prep |
EUR 461.08 |
- Product category: PCR Related/PCR Kits (Cleanup)
|
 Frit Kit |
|
FRIT-KIT |
Next Advance |
1each |
EUR 124 |
|
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
 Column Packing Kit |
|
PACK-KIT |
Next Advance |
1pack |
EUR 1035 |
|
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
 PCR Mycoplasma Detection Kit |
|
M034-Kit |
TOKU-E |
Kit |
EUR 266 |
 Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
|
CAS400A-KIT |
SBI |
1 kit (10 rxn) |
EUR 1110 |
|
|
 CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV100PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
 CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV105PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
 MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV120PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
 MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV125PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
 Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|
CAS700A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|
CAS720A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
|
CAS740A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
) T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
|
CAS510A-KIT |
SBI |
1 Kit |
EUR 805 |
|
|
-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV200PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV205PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV220PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|
CASLV225PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
 Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
|
CAS750A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
|
CAS770A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
|
CAS790A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
|
 and Fast ligase (2.5ul)]) Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
|
CAS9LIG-KIT |
SBI |
1 Kit |
EUR 153 |
|
|
) PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
|
PIN320A-KIT |
SBI |
1 Kit |
EUR 4941 |
- Category: PinPoint Integrase Tools
|
) PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
|
PIN340iPS-KIT |
SBI |
1 Kit |
EUR 4941 |
- Category: PinPoint Integrase Tools
|
, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)) AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|
GE620A-KIT |
SBI |
1 kit |
EUR 2132 |
|
|
, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)) AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|
GE622A-KIT |
SBI |
1 kit |
EUR 2132 |
|
|
, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)) AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|
GE624A-KIT |
SBI |
1 kit |
EUR 2132 |
|
|
) hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9) |
|
CAS620A-KIT |
SBI |
1 kit |
EUR 2152 |
|
|
|
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression) |
) PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
|
PIN300A-KIT |
SBI |
1 Kit |
EUR 2798 |
- Category: PinPoint Integrase Tools
|
) PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
|
PIN400A-KIT |
SBI |
1 Kit |
EUR 2798 |
- Category: PinPoint Integrase Tools
|
) PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
|
PIN410A-KIT |
SBI |
1 Kit |
EUR 4335 |
- Category: PinPoint Integrase Tools
|
) PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
|
PIN412A-KIT |
SBI |
1 Kit |
EUR 4335 |
- Category: PinPoint Integrase Tools
|
 vWF Acty. Kit |
|
ABP-ACT-KIT |
Abpcorp |
12 x 8 microwells |
EUR 428 |
 vWF Ant. Kit |
|
ABP-TOT-KIT |
Abpcorp |
12 x 8 microwells |
EUR 394 |
 PrecisionX Multiplex gRNA Cloning Kit |
|
CAS9-GRNA-KIT |
SBI |
10 rxn |
EUR 445 |
|
|
) mRNAExpress mRNA Synthesis kit (5 reactions) |
|
MR-KIT-1 |
SBI |
5 reactions |
EUR 1152 |
- Category: Stem Cell Products
|
 with goat anti-rabbit HRP secondary antibody) ExoAb Antibody Kit (CD9, CD63, CD81, Hsp70 antibodies, rabbit anti-human) with goat anti-rabbit HRP secondary antibody |
|
EXOAB-KIT-1 |
SBI |
25 ul each |
EUR 627 |
|
|
) CLOuD9 Gene Expression Regulation Kit (includes 10 ug each of dCas9-PYL1 and dCas9-ABI1 lentivectors, and 100 ul of 0.5M Inducer Agent) |
|
CASCL9-100A-KIT |
SBI |
1 Kit |
EUR 1132 |
|
|
 AXYGEN® AXYPET® PRO STARTER KIT: 4 PIPETTORS, PLEXI STAND, SAMPLE TIPS |
|
AP-STR-KIT-P |
CORNING |
1/pk |
EUR 721 |
|
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
 AXYPET STARTER KIT 1 AP-20, AP-200 & AP-1000 WITH ADDITIONAL FREE RACKS OF AXYGEN PIPETTE TIPS |
|
AP-STR-KIT-1 |
CORNING |
1/pk |
EUR 355 |
|
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
 AXYPET STARTER KIT 2 AP-10, AP-100 & AP-1000 WITH ADDITIONAL FREE RACKS OF AXYGEN PIPETTE TIPS |
|
AP-STR-KIT-2 |
CORNING |
1/pk |
EUR 367 |
|
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller |
 SEPTA MAT, FOR 96 WELL PCR PLATES, SILICONE, GREY, NONSTERILE, FOR ABI MULTI-CAPILLARY SEQUENCING INSTRUMENTS, BULK |
|
AM-96-SEPTA-3100 |
CORNING |
10/pk |
EUR 533 |
|
Description: Sealing Products; Sealing mats - Axygen |
 ELISA kit for Pregnenolone Kit |
|
E-EL-0086 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 377 |
- Gentaur's Pregnenolone ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Pregnenolone. During the reaction, Pregnenolone in the sample or standard competes with a fixed amount of P
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|
|
Description: A competitive ELISA kit for quantitative measurement of Pregnenolone Kit in samples from Serum, Plasma, Cell supernatant |
 Anti-c-Kit/Kit Antibody |
|
A01335-1 |
BosterBio |
100ug/vial |
EUR 334 |
 Human KIT/SCFR ELISA kit |
|
LF-EK50791 |
Abfrontier |
1×96T |
EUR 648 |
 (OKCD06003)) KIT ELISA Kit (Human) (OKCD06003) |
|
OKCD06003 |
Aviva Systems Biology |
96 Wells |
EUR 648 |
|
Description: Description of target: This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.61ng/mL |
 (OKCD06004)) KIT ELISA Kit (Mouse) (OKCD06004) |
|
OKCD06004 |
Aviva Systems Biology |
96 Wells |
EUR 779 |
|
Description: Description of target: The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: < 0.063ng/mL |
 (OKEH07314)) KIT ELISA Kit (Bovine) (OKEH07314) |
|
OKEH07314 |
Aviva Systems Biology |
96 Wells |
EUR 1092 |
|
Description: Description of target: ;Species reactivity: Bovine;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.15ng/mL |
 (OKEH07315)) KIT ELISA Kit (Chicken) (OKEH07315) |
|
OKEH07315 |
Aviva Systems Biology |
96 Wells |
EUR 1184 |
|
Description: Description of target: ;Species reactivity: Chicken;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.15ng/mL |
 (OKEH07316)) KIT ELISA Kit (Dog) (OKEH07316) |
|
OKEH07316 |
Aviva Systems Biology |
96 Wells |
EUR 1184 |
|
Description: Description of target: ;Species reactivity: Dog;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.15ng/mL |
 (OKEH07317)) KIT ELISA Kit (Pig) (OKEH07317) |
|
OKEH07317 |
Aviva Systems Biology |
96 Wells |
EUR 1092 |
|
Description: Description of target: ;Species reactivity: Pig;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.15ng/mL |
 (OKBB01105)) Kit ELISA Kit (Mouse) (OKBB01105) |
|
OKBB01105 |
Aviva Systems Biology |
96 Wells |
EUR 505 |
|
Description: Description of target: SCFR(Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.;Species reactivity: Mouse;Application: ELISA;Assay info: ;Sensitivity: <10pg/ml |
 (OKAN04574)) KIT ELISA Kit (Human) (OKAN04574) |
|
OKAN04574 |
Aviva Systems Biology |
96 Wells |
EUR 792 |
|
Description: Description of target: This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.61 ng/mL |
 Kit) ELISA kit for BH4 (Tetrahydrobiopterin) Kit |
|
E-EL-0110 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 377 |
- Gentaur's BH4 ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BH4. During the reaction, BH4 in the sample or standard competes with a fixed amount of BH4 on the solid phase suppo
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|
Description: A competitive ELISA kit for quantitative measurement of BH4 (Tetrahydrobiopterin) Kit in samples from Serum, Plasma, Cell supernatant |
 Kit) ELISA kit for BH2 (Dihydrobiopterin) Kit |
|
E-EL-0111 |
Elabscience Biotech |
1 plate of 96 wells |
EUR 377 |
- Gentaur's BH2 ELISA kit utilizes the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with BH2. During the reaction, BH2 in the sample or standard competes with a fixed amount of BH2 on the solid phase suppo
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|
|
Description: A competitive ELISA kit for quantitative measurement of BH2 (Dihydrobiopterin) Kit in samples from Serum, Plasma, Cell supernatant |
 Mouse Kit Ligand, Kitl ELISA Kit |
|
DEIA140 |
Creative Diagnostics |
10 plates |
EUR 2495 |
|
Description: Mouse Kitl ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant SCF in a sandwich ELISA format within the range of 32-5,250 pg/mL. Using the ELISA protocol described below, this kit provides sufficient reagents to assay Kitl in approximately 1,500 ELISA plate wells. |
 Human Kit Ligand, KitLG ELISA Kit |
|
DEIA141 |
Creative Diagnostics |
10 plates |
EUR 2495 |
|
Description: Human KITLG ELISA development kit contains the key components required for the quantitative measurement of natural and/or recombinant KITLG in a sandwich ELISA format within the range of 16-2,000 pg/mL. Using the ELISA protocol described below, this kit provides sufficient reagents to assay KITLG in approximately 1,000 ELISA plate wells |
 Rat Kit Ligand, Kitlg ELISA Kit |
|
DEIA142 |
Creative Diagnostics |
10 plates |
EUR 2495 |
|
Description: The Human DKK1 ELISA kit is for the quantitative determination of Human DKK1.;This ELISA kit contains the basic components required for the development of sandwich ELISAs. Each kit contains sufficient materials to run ELISAs on five 96-well plates. |
 Bovine KITLG/ Kit ligand ELISA Kit |
|
E0164Bo |
Sunlong |
1 Kit |
EUR 717 |
 Rat Kitlg/ Kit ligand ELISA Kit |
|
E0545Ra |
Sunlong |
1 Kit |
EUR 546 |
 ELISA Kit) Human Kit ligand (KITLG) ELISA Kit |
|
abx253483-96tests |
Abbexa |
96 tests |
EUR 668 |
- Shipped within 5-12 working days.
|
We present the effectivity of the proposed workflow by profitable droplet digital PCR amplification of each cell-free DNA and RNA and by detection of tumor-specific alterations in low-coverage whole-genome sequencing and DNA methylation profiling of plasma-derived cell-free DNA. For the primary time, we demonstrated profitable parallel extraction of cell-free DNA and RNA from plasma samples. This workflow paves the highway in the direction of multi-layer genomic evaluation from one single liquid biopsy pattern.
