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Deploying new generation sequencing for the study of flesh color depletion in Atlantic Salmon (Salmo salar)

Deploying new generation sequencing for the study of flesh color depletion in Atlantic Salmon (Salmo salar)

Posted on July 23, 2021 by Vasil

The flesh pigmentation of farmed Atlantic salmon is fashioned by accumulation of carotenoids derived from industrial diets. In the salmon gastrointestinal system, the hindgut is taken into account important in the processes of carotenoids uptake and metabolism. In Tasmania, flesh color depletion can noticeably have an effect on farmed Atlantic salmon at completely different ranges of severity following extraordinarily sizzling summers.

In this study, RNA sequencing (RNA-Seq) was carried out to research the discount in flesh pigmentation. Library preparation is a key step that considerably impacts the effectiveness of RNA sequencing (RNA-Seq) experiments. Besides the generally used complete transcript RNA-Seq methodology, the 3′ mRNA-Seq methodology is being utilized extensively, owing to its diminished value, enabling extra repeats to be sequenced at the expense of decrease decision.

Therefore, the output of the Illumina TruSeq equipment (complete transcript RNA-Seq) and the Lexogen QuantSeq equipment (3′ mRNA-Seq) was analyzed to establish genes in the Atlantic salmon hindgut which are differentially expressed (DEGs) between two flesh color phenotypes. each strategies, DEGs between the two color phenotypes have been related to metallic ion transport, oxidation-reduction processes, and immune responses.

We additionally discovered DEGs associated to lipid metabolism in the QuantSeq methodology. In the TruSeq methodology, a missense mutation was detected in DEGs in completely different flesh color traits. The quantity of DEGs discovered in the TruSeq libraries was a lot larger than the QuantSeq; nonetheless, the pattern of DEGs in each library strategies was related and validated by qPCR.

First Report of Little cherry virus 1 Infecting Sweet Cherry in Ontario, Canada

The Niagara fruit belt is one of the richest fruit-producing areas in Canada, contributing to 90% of Ontario’s tender fruits reminiscent of peach, plum and candy cherry. Little cherry virus 1 (LCV1) of the genus Velarivirus is a causal agent of little cherry illness which has devastated cherry crops in many areas (Eastwell and Bernardy 1998, Jelkmann and Eastwell, 2011).

From 2013 to 2018, foliar signs indicative of viral an infection reminiscent of leaf deformation, ringspot, mottling, vein clearing, and reddening have been discovered on candy cherry bushes grown in the Niagara area. To decide if these bushes have been contaminated by a virus, small RNAs (sRNAs) have been remoted from individually pooled asymptomatic and symptomatic leaves utilizing the mirPremier microRNA isolation equipment (Sigma Aldrich Canada, Oakville, ON).

The sRNAs have been used to create two libraries (4 leaves per library) with the TruSeq Small RNA Sample Prep Kit (Illumina, San Diego, CA). The sRNA libraries have been individually sequenced with the MiSeq Desktop Sequencer (Illumina, San Diego, CA). In whole, 5,380,196 reads have been obtained and Trimmomatic (Bolger et al. 2014) was used to take away adaptors.

The remaining 4,733,804 clear reads have been assembled into contigs utilizing Velvet 0.7.31 and Oases 0.2.09 (Schulz et al. 2012) with minimal size of 75 nt (Supplementary Table 1). A BLASTn search (Altschul et al. 1997) of the contigs recognized the presence of Cherry virus A (genus: Capillovirus), two members of the Ilarvirus genus (Prunus necrotic ringspot virus and Prune dwarf virus) in each libraries. LCV1 was solely discovered in contigs derived from the symptomatic library.

Of the clear reads, 22,016 have been assembled into six contigs (with lengths starting from 86 to 116 nt, Supplementary Table 1) mapping to LCV1, protecting 7.07% of the viral genome. To affirm LCV1 an infection, primers have been designed from the assembled contigs and used for reverse transcription polymerase chain response (RT-PCR).

Amplicons have been sequenced and the terminal sequences have been decided utilizing 5′ and three’ RACE Systems (Invitrogen, Burlington, ON). Degenerate primers have been designed from a number of sequence alignments of printed LCV1 genomes for amplification and primer strolling to acquire the sequence of LCV1 (Table S2). The full genome sequence of LCV1 has a size of 16,934 nt and was deposited in GenBank .

Deploying new generation sequencing for the study of flesh color depletion in Atlantic Salmon (Salmo salar)

A BLASTn search confirmed that this isolate is sort of similar (99.6% sequence identification) to an isolate from California . To decide the incidence of an infection, a discipline survey was carried out at the similar location throughout spring months of 2014 to 2018 utilizing RT-PCR with primers particular to the viral coat protein gene (Supplementary Tale 2).

Among 46 cherry bushes sampled, two (4.3%) bushes have been contaminated with LCV1 and confirmed adverse outcomes with CVA, PNRSV and PDV. Both bushes displayed delicate suturing of major and secondary veins (Supplementary Figure 1).

LCV1 has been recognized in Western stone fruit producing areas (British Columbia in Canada, and Washington, California, and Oregon in the United States of America). To the greatest of our data, that is the first report of LCV1 in any japanese area of Canada. The low incidence of LCV1 means that this virus shouldn’t be widespread in this area. Routine monitoring and detection of LCV1 is required to forestall this devastating cherry illness from spreading in this area.

Streptococcus, Centrocestus formosanus and Myxobolus tilapiae concurrent infections in farmed Nile tilapia (Oreochromis niloticus)

Stress triggered concurrent microbial/parasitic infections are prevalent in earthen pond based mostly farmed Nile tilapia Oreochromis niloticus. In the present study, a complete of thirty 5 O. niloticus have been collected from a industrial fish farm with a historical past of extreme mortalities at Port Said, Egypt. Nile tilapia samples have been subjected to bacteriological, parasitological and pathological examinations.

Twenty one Enterococcus fecalis and 15 Streptococcus agalactiae isolates have been presumptively recognized using the semi-automated API 20 Strept take a look at equipment. The identities of the retrieved micro organism have been confirmed by the sequencing of 16 S rRNA gene.

Moribund O. niloticus have been discovered to be closely contaminated by one or each of Centrocestus formosanus encysted metacercariae (EMC) and/or Myxobolus tilapiae spores presenting a singular type of synergistic and/or symbiotic relationship.

The identities of each parasites have been confirmed by morphological and molecular characterization. Variable circulatory, degenerative, necrotic and proliferative modifications have been additionally seen in hematopoietic organs. Interestingly, a number of myxobolus spores and EMC have been seen in some histological sections.

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It was apparent that the present concurrent bacterial and parasitic infections are triggered by the deleterious results of some stressing environmental circumstances. The unfavorable weather conditions (excessive temperature and excessive relative humidity) recorded at the surge of mortalities are possible predisposing stress elements.

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  • Dog GMCSF(Colony Stimulating Factor 2, Granulocyte Macrophage) ELISA Kit
  • Dog ICAM1(Intercellular Adhesion Molecule 1) ELISA Kit
  • Dog IFNa(Interferon Alpha) ELISA Kit
  • Dog IFNg(Interferon Gamma) ELISA Kit
  • Dog IL10(Interleukin 10) ELISA Kit
  • Dog IL17(Interleukin 17) ELISA Kit
  • Dog IL18(Interleukin 18) ELISA Kit
  • Dog IL2(Interleukin 2) ELISA Kit
  • Dog IL4(Interleukin 4) ELISA Kit
  • Dog IL6(Interleukin 6) ELISA Kit
  • Dog OPN(Osteopontin) ELISA Kit
  • Dog REN(Renin) ELISA Kit
  • Dog SPA(Surfactant Associated Protein A) ELISA Kit
  • Dog TGFb1(Transforming Growth Factor Beta 1) ELISA Kit
  • Dog TGFb2(Transforming Growth Factor Beta 2) ELISA Kit
  • Guinea pig CRP(C Reactive Protein) ELISA Kit
  • Guinea pig EDN1(Endothelin 1) ELISA Kit
  • Guinea pig IFNg(Interferon Gamma) ELISA Kit
  • Guinea pig IL10(Interleukin 10) ELISA Kit
  • Guinea pig IL5(Interleukin 5) ELISA Kit
  • Human 20S-PSM(20S-Proteasome) ELISA Kit
  • Human ABCD1(ATP Binding Cassette Transporter D1) ELISA Kit
  • Human ACTN1(Actinin Alpha 1) ELISA Kit
  • Human ACVA(Activin A) ELISA Kit
  • Human ADH(Antidiuretic Hormone) ELISA Kit
  • Human ADIG(Adipogenin) ELISA Kit
  • Human ADPRH(ADP Ribosylarginine Hydrolase) ELISA Kit
  • Human ADRb1(Adrenergic Receptor Beta 1) ELISA Kit
  • Human ADRb3(Adrenergic Receptor Beta 3) ELISA Kit
  • Human ALDOS(Aldosterone Synthase) ELISA Kit
  • Human AMY2(Amylase Alpha 2, Pancreatic) ELISA Kit
  • Human ANKRD12(Ankyrin Repeat Domain Protein 12) ELISA Kit
  • Human ANP(Atrial Natriuretic Peptide) ELISA Kit
  • Human ANXA7(Annexin A7) ELISA Kit
  • Human AOPP(Advanced Oxidation Protein Products) ELISA Kit
  • Human AP3b1(Adaptor Related Protein Complex 3 Beta 1) ELISA Kit
  • Human AQP5(Aquaporin 5) ELISA Kit
  • Human ARNTL(Aryl Hydrocarbon Receptor Nuclear Translocator Like Protein) ELISA Kit
  • Human AS3MT(Arsenic, 3-Oxidation State Methyltransferase) ELISA Kit
  • Human ATF1(Activating Transcription Factor 1) ELISA Kit
  • Human ATP6AP2(ATPase, H+ Transporting, Lysosomal Accessory Protein 2) ELISA Kit
  • Human BCAS4(Breast Carcinoma Amplified Sequence 4) ELISA Kit
  • Human Bcl9L(B-Cell CLL/Lymphoma 9 Like Protein) ELISA Kit
  • Human BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
  • Human BNP(Brain Natriuretic Peptide) ELISA Kit
  • Human BRD4(Bromodomain Containing Protein 4) ELISA Kit
  • Human cADPRH(Cyclic ADP Ribose Hydrolase) ELISA Kit
  • Human CAR(Constitutive Androstane Receptor) ELISA Kit
  • Human CASP11(Caspase 11) ELISA Kit
  • Human CCK25(Cholecystokinin 25) ELISA Kit
  • Human CCL16(Chemokine C-C-Motif Ligand 16) ELISA Kit
  • Human CCNA(Cyclin A) ELISA Kit
  • Human CCND3(Cyclin D3) ELISA Kit
  • Human CGa(Chorionic Gonadotropin Alpha Polypeptide) ELISA Kit
  • Human CGb(Chorionic Gonadotropin Beta Polypeptide) ELISA Kit
  • Human CGb(Chorionic Gonadotropin Beta Polypeptide) ELISA Kit
  • Human CHRD(Chordin) ELISA Kit
  • Human CLDN18(Claudin 18) ELISA Kit
  • Human CLDN2(Claudin 2) ELISA Kit
  • Human CLDN8(Claudin 8) ELISA Kit
  • Human CLL1(Collectin Liver 1) ELISA Kit
  • Human CNP(C-Type Natriuretic Peptide) ELISA Kit
  • Human COCH(Cochlin) ELISA Kit
  • Human COL4a5(Collagen Type IV Alpha 5) ELISA Kit
  • Human CPLX2(Complexin 2) ELISA Kit
  • Human CPLX2(Complexin 2) ELISA Kit
  • Human CPP(Copeptin) ELISA Kit
  • Human CPP(Copeptin) ELISA Kit
  • Human CRT(Calreticulin) ELISA Kit
  • Human CRYbB2(Crystallin Beta B2) ELISA Kit
  • Human CST5(Cystatin 5) ELISA Kit
  • Human CYCS(Cytochrome C, Somatic) ELISA Kit
  • Human CYP21B(Cytochrome P450 21B) ELISA Kit
  • Human CYP2B6(Cytochrome P450 2B6) ELISA Kit
  • Human CYP2C18(Cytochrome P450 2C18) ELISA Kit
  • Human CYP2C19(Cytochrome P450 2C19) ELISA Kit
  • Human CYP2D6(Cytochrome P450 2D6) ELISA Kit
  • Human CYP46A1(Cytochrome P450 46A1) ELISA Kit
  • Human CYPD(Cyclophilin D) ELISA Kit
  • Human CYS1(Cystin 1) ELISA Kit
  • Human CYSLTR2(Cysteinyl Leukotriene Receptor 2) ELISA Kit
  • Human CYTL1(Cytokine Like Protein 1) ELISA Kit
  • Human DAPP1(Dual Adaptor Of Phosphotyrosine And 3-Phosphoinositides) ELISA Kit
  • Human DDOST(Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase) ELISA Kit
  • Human DIO1(Deiodinase, Iodothyronine, Type I) ELISA Kit
  • Human DKK3(Dickkopf Related Protein 3) ELISA Kit
  • Human DLC1(Deleted In Liver Cancer 1) ELISA Kit
  • Human DYNC2H1(Dynein, Cytoplasmic 2, Heavy Chain 1) ELISA Kit
  • Human ES(Endostatin) ELISA Kit
  • Human FADD(Fas Associating Death Domain Containing Protein) ELISA Kit
  • Human FAM3A(Family With Sequence Similarity 3, Member A) ELISA Kit
  • Human FGF13(Fibroblast Growth Factor 13) ELISA Kit
  • Human FGF18(Fibroblast Growth Factor 18) ELISA Kit
  • Human FGF2(Fibroblast Growth Factor 2, Basic) ELISA Kit
  • Human FGF20(Fibroblast Growth Factor 20) ELISA Kit
  • Human FLOT1(Flotillin 1) ELISA Kit
  • Human FNTb(Farnesyltransferase Beta) ELISA Kit
  • Human FOLR2(Folate Receptor 2, Fetal) ELISA Kit
  • Human FOXP1(Forkhead Box Protein P1) ELISA Kit
  • Human FOXQ1(Forkhead Box Protein Q1) ELISA Kit
  • Human FPN(Ferroportin) ELISA Kit
  • Human FUT2(Fucosyltransferase 2) ELISA Kit
  • Human GAL12(Galectin 12) ELISA Kit
  • Human gEP(Gamma-Endorphin) ELISA Kit
  • Human GH(Growth Hormone) ELISA Kit
  • Human GH(Growth Hormone) ELISA Kit
  • Human GLP1(Glucagon Like Peptide 1) ELISA Kit
  • Human GluAP(Glutamyl Aminopeptidase) ELISA Kit
  • Human GnRH(Gonadotropin Releasing Hormone) ELISA Kit
  • Human GRId2(Glutamate Receptor, Ionotropic, Delta 2) ELISA Kit
  • Human GRIN1(Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 1) ELISA Kit
  • Human GSDMD(Gasdermin D) ELISA Kit
  • Human GUCA2A(Guanylate Cyclase Activator 2A) ELISA Kit
  • Human H1(Histone H1) ELISA Kit
  • Human HbA1c(Glycated Hemoglobin A1c) ELISA Kit
  • Human HbA1c(Glycated Hemoglobin A1c) ELISA Kit
  • Human HBd(Hemoglobin Delta) ELISA Kit
  • Human HCK(Hemopoietic Cell Kinase) ELISA Kit
  • Human HCN4(Hyperpolarization Activated Cyclic Nucleotide Gated Potassium Channel 4) ELISA Kit
  • Human HJV(Hemojuvelin) ELISA Kit
  • Human HJV(Hemojuvelin) ELISA Kit
  • Human HPEPT1(Hydrogen Ion/Peptide Transporter 1) ELISA Kit
  • Human HPEPT2(Hydrogen Ion/Peptide Transporter 2) ELISA Kit
  • Human HSP90(Heat Shock Protein 90) ELISA Kit
  • Human HSPA1L(Heat Shock 70kDa Protein 1 Like Protein) ELISA Kit
  • Human Igk(Immunoglobulin Kappa) ELISA Kit
  • Human IkBKg(Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Kinase Gamma) ELISA Kit
  • Human IL22R(Interleukin 22 Receptor) ELISA Kit
  • Human IMA(Ischemia Modified Albumin) ELISA Kit
  • Human INHbA(Inhibin Beta A) ELISA Kit
  • Human IRAK2(Interleukin 1 Receptor Associated Kinase 2) ELISA Kit
  • Human IRS2(Insulin Receptor Substrate 2) ELISA Kit
  • Human JAZF1(Juxtaposed With Another Zinc Finger Protein 1) ELISA Kit
  • Human JTB(Jumping Translocation Breakpoint Protein) ELISA Kit
  • Human KISS1(Kisspeptin 1) ELISA Kit
  • Human KLK6(Kallikrein 6) ELISA Kit
  • Human LAB7-1(B-Lymphocyte Activation Antigen B7-1) ELISA Kit
  • Human LAMa2(Laminin Alpha 2) ELISA Kit
  • Human LH(Luteinizing Hormone) ELISA Kit
  • Human LHb(Luteinizing Hormone Beta Polypeptide) ELISA Kit
  • Human LMP7(Large Multifunctional Peptidase 7) ELISA Kit
  • Human LPIN1(Lipin 1) ELISA Kit
  • Human MAOA(Monoamine Oxidase A) ELISA Kit
  • Human MAPK9(Mitogen Activated Protein Kinase 9) ELISA Kit
  • Human MCCC2(Methylcrotonoyl Coenzyme A Carboxylase 2) ELISA Kit
  • Human MCP1(Monocyte Chemotactic Protein 1) ELISA Kit
  • Human MEFV(Mediterranean Fever) ELISA Kit
  • Human MEP1a(Meprin A Alpha) ELISA Kit
  • Human MPL(Myeloproliferative Leukemia Virus Oncogene) ELISA Kit
  • Human MSTN(Myostatin) ELISA Kit
  • Human MVD(Mevalonate Decarboxylase) ELISA Kit
  • Human NCOR1(Nuclear Receptor Corepressor 1) ELISA Kit
  • Human NEFL(Neurofilament, Light Polypeptide) ELISA Kit
  • Human NIPA1(Non Imprinted In Prader Willi/Angelman Syndrome Region Protein 1) ELISA Kit
  • Human NKAP(NFKB Activating Protein) ELISA Kit
  • Human NKX3-1(NK3 Homeobox Protein 1) ELISA Kit
  • Human NLRP3(NLR Family, Pyrin Domain Containing Protein 3) ELISA Kit
  • Human NOD2(Nucleotide Binding Oligomerization Domain Containing Protein 2) ELISA Kit
  • Human NOG(Noggin) ELISA Kit
  • Human NR1D1(Nuclear Receptor Subfamily 1, Group D, Member 1) ELISA Kit
  • Human NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
  • Human OB(Obestatin) ELISA Kit
  • Human OB(Obestatin) ELISA Kit
  • Human OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit
  • Human PDHb(Pyruvate Dehydrogenase Beta) ELISA Kit
  • Human PEX2(Peroxisomal Biogenesis Factor 2) ELISA Kit
  • Human PI(Proinsulin) ELISA Kit
  • Human PIGL(Phosphatidylinositol Glycan L) ELISA Kit
  • Human PIICP(Procollagen II C-Terminal Propeptide) ELISA Kit
  • Human PIIICP(Procollagen III C-Terminal Propeptide) ELISA Kit
  • Human PIIINP(Procollagen III N-Terminal Propeptide) ELISA Kit
  • Human PIN1(Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 1) ELISA Kit
  • Human PINP(Procollagen I N-Terminal Propeptide) ELISA Kit
  • Human PINP(Procollagen I N-Terminal Propeptide) ELISA Kit
  • Human PLK3(Polo Like Kinase 3) ELISA Kit
  • Human PNPLA2(Patatin Like Phospholipase Domain Containing Protein 2) ELISA Kit
  • Human PP(Pancreatic Polypeptide) ELISA Kit
  • Human PPM1B(Protein Phosphatase, Mg2+/Mn2+ Dependent 1B) ELISA Kit
  • Human PRMT1(Protein Arginine Methyltransferase 1) ELISA Kit
  • Human ProNT/NMN(Proneurotensin/neuromedin N) ELISA Kit
  • Human PSGL1(P-Selectin Glycoprotein Ligand 1) ELISA Kit
  • Human PSMb10(Proteasome Subunit Beta Type 10) ELISA Kit
  • Human PTHrP(Parathyroid Hormone Related Protein) ELISA Kit
  • Human PTPRF(Protein Tyrosine Phosphatase Receptor Type F) ELISA Kit
  • Human PTPRZ(Protein Tyrosine Phosphatase Receptor Type Z) ELISA Kit
  • Human RASSF1(Ras Association Domain Containing Protein 1) ELISA Kit
  • Human RBM8(RNA Binding Motif Protein 8) ELISA Kit
  • Human RHD(Rh Blood Group, D Antigen) ELISA Kit
  • Human Rock2(Rho Associated Coiled Coil Containing Protein Kinase 2) ELISA Kit
  • Human RORa(RAR Related Orphan Receptor Alpha) ELISA Kit
  • Human RPS9(Ribosomal Protein S9) ELISA Kit
  • Human RUNX3(Runt Related Transcription Factor 3) ELISA Kit
  • Human S100A4(S100 Calcium Binding Protein A4) ELISA Kit
  • Human S100A4(S100 Calcium Binding Protein A4) ELISA Kit
  • Human SAA(Serum Amyloid A) ELISA Kit
  • Human SEPT8(Septin 8) ELISA Kit
  • Human SESN3(Sestrin 3) ELISA Kit
  • Human SLC39A4(Solute Carrier Family 39, Member 4) ELISA Kit
  • Human SLC6A19(Solute Carrier Family 6, Member 19) ELISA Kit
  • Human SLC7A11(Solute Carrier Family 7, Member 11) ELISA Kit
  • Human Slit3(Slit Homolog 3) ELISA Kit
  • Human Smad1(Mothers Against Decapentaplegic Homolog 1) ELISA Kit
  • Human Smad2(Mothers Against Decapentaplegic Homolog 2) ELISA Kit
  • Human Smad5(Mothers Against Decapentaplegic Homolog 5) ELISA Kit
  • Human Smad9(Mothers Against Decapentaplegic Homolog 9) ELISA Kit
  • Human SMARCC1(SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily C, Member 1) ELISA Kit
  • Human SNAP25(Synaptosomal Associated Protein 25kDa) ELISA Kit
  • Human SOD(Superoxide Dismutases) ELISA Kit
  • Human SPAG9(Sperm Associated Antigen 9) ELISA Kit
  • Human SPRR4(Small Proline Rich Protein 4) ELISA Kit
  • Human STAT1(Signal Transducer And Activator Of Transcription 1) ELISA Kit
  • Human STAT3(Signal Transducer And Activator Of Transcription 3) ELISA Kit
  • Human STMN1(Stathmin 1) ELISA Kit
  • Human SYN1(Synapsin I) ELISA Kit
  • Human SYP(Synaptophysin) ELISA Kit
  • Human SYT7(Synaptotagmin VII) ELISA Kit
  • Human TACTILE(T-Cell Activation Antigen, Increased Late Expression) ELISA Kit
  • Human TAN1(Translocation Associated Notch Homolog 1) ELISA Kit
  • Human TAUT(Taurine Transporter) ELISA Kit
  • Human TGFb2(Transforming Growth Factor Beta 2) ELISA Kit
  • Human TNP1(Transition Protein 1) ELISA Kit
  • Human TrxR2(Thioredoxin Reductase 2) ELISA Kit
  • Human TSPAN6(Tetraspanin 6) ELISA Kit
  • Human TUBb1(Tubulin Beta 1) ELISA Kit
  • Human Ub(Ubiquitin) ELISA Kit
  • Human UCHL1(Ubiquitin Carboxyl Terminal Hydrolase L1) ELISA Kit
  • Human UCN2(Urocortin 2) ELISA Kit
  • Human UROC1(Urocanase Domain Containing Protein 1) ELISA Kit
  • Human UTRN(Utrophin) ELISA Kit
  • Human UXS1(UDP Glucuronate Decarboxylase 1) ELISA Kit
  • Human VASH1(Vasohibin 1) ELISA Kit
  • Human VF(Visfatin) ELISA Kit
  • Human vWF(Von Willebrand Factor) ELISA Kit
  • Human WNT3A(Wingless Type MMTV Integration Site Family, Member 3A) ELISA Kit
  • Human WNT5A(Wingless Type MMTV Integration Site Family, Member 5A) ELISA Kit
  • Human ZFHX4(Zinc Finger Homeobox Protein 4) ELISA Kit
  • Mouse a1M(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
  • Mouse ACVA(Activin A) ELISA Kit
  • Mouse ADAM17(A Disintegrin And Metalloprotease 17) ELISA Kit
  • Mouse aHSP(Alpha-Hemoglobin Stabilizing Protein) ELISA Kit
  • Mouse aMSH(Alpha-Melanocyte Stimulating Hormone) ELISA Kit
  • Mouse AngIII(Angiotensin III) ELISA Kit
  • Mouse ANP(Atrial Natriuretic Peptide) ELISA Kit
  • Mouse BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
  • Mouse BNP(Brain Natriuretic Peptide) ELISA Kit
  • Mouse C3c(Complement C3 Convertase) ELISA Kit
  • Mouse C4(Complement Component 4) ELISA Kit
  • Mouse CA2(Carbonic Anhydrase II) ELISA Kit
  • Mouse CAMP(Cathelicidin Antimicrobial Peptide) ELISA Kit
  • Mouse CaN(Calcineurin) ELISA Kit
  • Mouse CAPNS1(Calpain, Small Subunit 1) ELISA Kit
  • Mouse CASP14(Caspase 14) ELISA Kit
  • Mouse CAV1(Caveolin 1) ELISA Kit
  • Mouse CCR2(Chemokine C-C-Motif Receptor 2) ELISA Kit
  • Mouse CD8a(Cluster Of Differentiation 8a) ELISA Kit
  • Mouse CD99(Cluster Of Differentiation 99) ELISA Kit
  • Mouse CKB(Creatine Kinase, Brain) ELISA Kit
  • Mouse CXCL16(Chemokine C-X-C-Motif Ligand 16) ELISA Kit
  • Mouse CYSLTR2(Cysteinyl Leukotriene Receptor 2) ELISA Kit
  • Mouse DAO(Diamine Oxidase) ELISA Kit
  • Mouse DAT(Dopamine Transporter) ELISA Kit
  • Mouse F13A1(Coagulation Factor XIII A1 Polypeptide) ELISA Kit
  • Mouse FABP7(Fatty Acid Binding Protein 7, Brain) ELISA Kit
  • Mouse FGa(Fibrinogen Alpha) ELISA Kit
  • Mouse FGF2(Fibroblast Growth Factor 2, Basic) ELISA Kit
  • Mouse FGF21(Fibroblast Growth Factor 21) ELISA Kit
  • Mouse GDF15(Growth Differentiation Factor 15) ELISA Kit
  • Mouse GnRH(Gonadotropin Releasing Hormone) ELISA Kit
  • Mouse GSTm1(Glutathione S Transferase Mu 1) ELISA Kit
  • Mouse GYS2(Glycogen Synthase 2, Liver) ELISA Kit
  • Mouse HPX(Hemopexin) ELISA Kit
  • Mouse IGFBP2(Insulin Like Growth Factor Binding Protein 2) ELISA Kit
  • Mouse IGFBP6(Insulin Like Growth Factor Binding Protein 6) ELISA Kit
  • Mouse IgM(Immunoglobulin M) ELISA Kit
  • Mouse IL34(Interleukin 34) ELISA Kit
  • Mouse IL35(Interleukin 35) ELISA Kit
  • Mouse INHA(Inhibin A) ELISA Kit
  • Mouse INHB(Inhibin B) ELISA Kit
  • Mouse INHbB(Inhibin Beta B) ELISA Kit
  • Mouse ITGb1(Integrin Beta 1) ELISA Kit
  • Mouse LAG3(Lymphocyte Activation Gene 3) ELISA Kit
  • Mouse LDHB(Lactate Dehydrogenase B) ELISA Kit
  • Mouse LIPE(Lipase, Hormone Sensitive) ELISA Kit
  • Mouse LNGFR(Low Affinity Nerve Growth Factor Receptor) ELISA Kit
  • Mouse MEP1a(Meprin A Alpha) ELISA Kit
  • Mouse MSTN(Myostatin) ELISA Kit
  • Mouse NEF3(Neurofilament 3) ELISA Kit
  • Mouse NEFL(Neurofilament, Light Polypeptide) ELISA Kit
  • Mouse NES1(Nesfatin 1) ELISA Kit
  • Mouse NNE(Enolase, Non Neuronal) ELISA Kit
  • Mouse NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
  • Mouse NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
  • Mouse OB(Obestatin) ELISA Kit
  • Mouse ORM2(Orosomucoid 2) ELISA Kit
  • Mouse Pax(Paxillin) ELISA Kit
  • Mouse PIICP(Procollagen II C-Terminal Propeptide) ELISA Kit
  • Mouse PIIICP(Procollagen III C-Terminal Propeptide) ELISA Kit
  • Mouse PINP(Procollagen I N-Terminal Propeptide) ELISA Kit
  • Mouse PINP(Procollagen I N-Terminal Propeptide) ELISA Kit
  • Mouse PP(Pancreatic Polypeptide) ELISA Kit
  • Mouse ProNT/NMN(Proneurotensin/neuromedin N) ELISA Kit
  • Mouse PTHrP(Parathyroid Hormone Related Protein) ELISA Kit
  • Mouse RNASE1(Ribonuclease A) ELISA Kit
  • Mouse RNASE3(Ribonuclease A3) ELISA Kit
  • Mouse SALa(Salusin Alpha) ELISA Kit
  • Mouse SALb(Salusin Beta) ELISA Kit
  • Mouse SEMA4D(Semaphorin 4D) ELISA Kit
  • Mouse SEPP1(Selenoprotein P1, Plasma) ELISA Kit
  • Mouse SPB(Surfactant Associated Protein B) ELISA Kit
  • Mouse SPC(Surfactant Associated Protein C) ELISA Kit
  • Mouse SYP(Synaptophysin) ELISA Kit
  • Mouse TFF1(Trefoil Factor 1) ELISA Kit
  • Mouse TLN(Talin) ELISA Kit
  • Mouse TLR9(Toll Like Receptor 9) ELISA Kit
  • Mouse UCN2(Urocortin 2) ELISA Kit
  • Mouse VEGF121(Vascular Endothelial Growth Factor 121) ELISA Kit
  • Mouse VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit
  • Mouse VF(Visfatin) ELISA Kit
  • Pig ACE(Angiotensin I Converting Enzyme) ELISA Kit
  • Pig ADP(Adiponectin) ELISA Kit
  • Pig aHSG(Alpha-2-Heremans Schmid Glycoprotein) ELISA Kit
  • Pig ANGPT2(Angiopoietin 2) ELISA Kit
  • Pig APOA2(Apolipoprotein A2) ELISA Kit
  • Pig C3(Complement Component 3) ELISA Kit
  • Pig CCK(Cholecystokinin) ELISA Kit
  • Pig COL3(Collagen Type III) ELISA Kit
  • Pig CRP(C Reactive Protein) ELISA Kit
  • Pig CST3(Cystatin 3) ELISA Kit
  • Pig D2D(D-Dimer) ELISA Kit
  • Pig DAO(Diamine Oxidase) ELISA Kit
  • Pig EGF(Epidermal Growth Factor) ELISA Kit
  • Pig FGF7(Fibroblast Growth Factor 7) ELISA Kit
  • Pig GH(Growth Hormone) ELISA Kit
  • Pig GHRH(Growth Hormone Releasing Hormone) ELISA Kit
  • Pig HIF1a(Hypoxia Inducible Factor 1 Alpha) ELISA Kit
  • Pig Hpt(Haptoglobin) ELISA Kit
  • Pig IFNa(Interferon Alpha) ELISA Kit
  • Pig IFNb(Interferon Beta) ELISA Kit
  • Pig IgE(Immunoglobulin E) ELISA Kit
  • Pig IGF1(Insulin Like Growth Factor 1) ELISA Kit
  • Pig IGF2(Insulin Like Growth Factor 2) ELISA Kit
  • Pig IGFBP3(Insulin Like Growth Factor Binding Protein 3) ELISA Kit
  • Pig IL12B(Interleukin 12B) ELISA Kit
  • Pig IL13(Interleukin 13) ELISA Kit
  • Pig IL17(Interleukin 17) ELISA Kit
  • Pig IL18(Interleukin 18) ELISA Kit
  • Pig IL1a(Interleukin 1 Alpha) ELISA Kit
  • Pig IL2(Interleukin 2) ELISA Kit
  • Pig IL4(Interleukin 4) ELISA Kit
  • Pig IL5(Interleukin 5) ELISA Kit
  • Pig LEP(Leptin) ELISA Kit
  • Pig MMP9(Matrix Metalloproteinase 9) ELISA Kit
  • Pig MPO(Myeloperoxidase) ELISA Kit
  • Pig NOS3(Nitric Oxide Synthase 3, Endothelial) ELISA Kit
  • Pig OC(Osteocalcin) ELISA Kit
  • Pig SELE(Selectin, Endothelium) ELISA Kit
  • Pig SELP(Selectin, Platelet) ELISA Kit
  • Pig SPA(Surfactant Associated Protein A) ELISA Kit
  • Pig SST(Somatostatin) ELISA Kit
  • Pig TF(Tissue Factor) ELISA Kit
  • Pig TGFb1(Transforming Growth Factor Beta 1) ELISA Kit
  • Pig TIMP1(Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit
  • Pig TM(Thrombomodulin) ELISA Kit
  • Pig TNNI3(Troponin I Type 3, Cardiac) ELISA Kit
  • Pig tPA(Plasminogen Activator, Tissue) ELISA Kit
  • Pig VEGFA(Vascular Endothelial Growth Factor A) ELISA Kit
  • Pig vWF(Von Willebrand Factor) ELISA Kit
  • Rabbit APOA1(Apolipoprotein A1) ELISA Kit
  • Rabbit FDP(Fibrinogen Degradation Product) ELISA Kit
  • Rabbit FSH(Follicle Stimulating Hormone) ELISA Kit
  • Rabbit Hpt(Haptoglobin) ELISA Kit
  • Rabbit IFNb(Interferon Beta) ELISA Kit
  • Rabbit LOX1(Lectin Like Oxidized Low Density Lipoprotein Receptor 1) ELISA Kit
  • Rabbit MMP10(Matrix Metalloproteinase 10) ELISA Kit
  • Rabbit NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
  • Rabbit S100A11(S100 Calcium Binding Protein A11) ELISA Kit
  • Rabbit SAA(Serum Amyloid A) ELISA Kit
  • Rabbit TLR9(Toll Like Receptor 9) ELISA Kit
  • Rat a1M(Alpha-1-Microglobulin/Bikunin Precursor) ELISA Kit
  • Rat ABCG2(ATP Binding Cassette Transporter G2) ELISA Kit
  • Rat ACE2(Angiotensin I Converting Enzyme 2) ELISA Kit
  • Rat ACVB(Activin B) ELISA Kit
  • Rat ADAM9(A Disintegrin And Metalloprotease 9) ELISA Kit
  • Rat ADM(Adrenomedullin) ELISA Kit
  • Rat AIF(Apoptosis Inducing Factor) ELISA Kit
  • Rat ALOX5(Arachidonate-5-Lipoxygenase) ELISA Kit
  • Rat ALP(Alkaline Phosphatase) ELISA Kit
  • Rat ALPL(Alkaline Phosphatase, Liver/Bone/Kidney) ELISA Kit
  • Rat AngIII(Angiotensin III) ELISA Kit
  • Rat ANP(Atrial Natriuretic Peptide) ELISA Kit
  • Rat ANXA2(Annexin A2) ELISA Kit
  • Rat APOB(Apolipoprotein B) ELISA Kit
  • Rat APOC1(Apolipoprotein C1) ELISA Kit
  • Rat AST(Aspartate Aminotransferase) ELISA Kit
  • Rat Bax(Bcl2 Associated X Protein) ELISA Kit
  • Rat BDNF(Brain Derived Neurotrophic Factor) ELISA Kit
  • Rat BNP(Brain Natriuretic Peptide) ELISA Kit
  • Rat C1INH(Complement 1 Inhibitor) ELISA Kit
  • Rat C1q(Complement 1q) ELISA Kit
  • Rat C3(Complement Component 3) ELISA Kit
  • Rat CA2(Carbonic Anhydrase II) ELISA Kit
  • Rat CACT(Carnitine Acylcarnitine Translocase) ELISA Kit
  • Rat CAMP(Cathelicidin Antimicrobial Peptide) ELISA Kit
  • Rat CaN(Calcineurin) ELISA Kit
  • Rat CAPNS1(Calpain, Small Subunit 1) ELISA Kit
  • Rat CCK8(Cholecystokinin 8, Octapeptide) ELISA Kit
  • Rat ChAT(Choline Acetyltransferase) ELISA Kit
  • Rat CHGA(Chromogranin A) ELISA Kit
  • Rat CLU(Clusterin) ELISA Kit
  • Rat COMP(Cartilage Oligomeric Matrix Protein) ELISA Kit
  • Rat CPP(Copeptin) ELISA Kit
  • Rat CSTA(Cystatin A) ELISA Kit
  • Rat CSTB(Cystatin B) ELISA Kit
  • Rat CYSLTR2(Cysteinyl Leukotriene Receptor 2) ELISA Kit
  • Rat DAT(Dopamine Transporter) ELISA Kit
  • Rat DEFb1(Defensin Beta 1) ELISA Kit
  • Rat Dyn(Big Dynorphin) ELISA Kit
  • Rat EMILIN1(Elastin Microfibril Interface Located Protein 1) ELISA Kit
  • Rat ERa(Estrogen Receptor Alpha) ELISA Kit
  • Rat FABP3(Fatty Acid Binding Protein 3, Muscle And Heart) ELISA Kit
  • Rat FETUB(Fetuin B) ELISA Kit
  • Rat FGa(Fibrinogen Alpha) ELISA Kit
  • Rat FGF10(Fibroblast Growth Factor 10) ELISA Kit
  • Rat FGF2(Fibroblast Growth Factor 2, Basic) ELISA Kit
  • Rat FGF23(Fibroblast Growth Factor 23) ELISA Kit
  • Rat FOS(V-Fos FBJ Murine Osteosarcoma Viral Oncogene Homolog) ELISA Kit
  • Rat FS(Follistatin) ELISA Kit
  • Rat GAL(Galanin) ELISA Kit
  • Rat GAL4(Galectin 4) ELISA Kit
  • Rat GDF15(Growth Differentiation Factor 15) ELISA Kit
  • Rat GnRH(Gonadotropin Releasing Hormone) ELISA Kit
  • Rat GS(Glutamine synthetase) ELISA Kit
  • Rat HAT1(Histone Acetyltransferase 1) ELISA Kit
  • Rat HbA1c(Glycated Hemoglobin A1c) ELISA Kit
  • Rat HDL(High Density Lipoprotein) ELISA Kit
  • Rat HPX(Hemopexin) ELISA Kit
  • Rat IDO(Indoleamine-2,3-Dioxygenase) ELISA Kit
  • Rat IL17B(Interleukin 17B) ELISA Kit
  • Rat IL21(Interleukin 21) ELISA Kit
  • Rat IL2Ra(Interleukin 2 Receptor Alpha) ELISA Kit
  • Rat IL6R(Interleukin 6 Receptor) ELISA Kit
  • Rat IL7R(Interleukin 7 Receptor) ELISA Kit
  • Rat IL8Rb(Interleukin 8 Receptor Beta) ELISA Kit
  • Rat INHA(Inhibin A) ELISA Kit
  • Rat INHB(Inhibin B) ELISA Kit
  • Rat ITGaM(Integrin Alpha M) ELISA Kit
  • Rat LBP(Lipopolysaccharide Binding Protein) ELISA Kit
  • Rat LDH(Lactate Dehydrogenase) ELISA Kit
  • Rat LOX1(Lectin Like Oxidized Low Density Lipoprotein Receptor 1) ELISA Kit
  • Rat LRG1(Leucine Rich Alpha-2-Glycoprotein 1) ELISA Kit
  • Rat MAP1A(Microtubule Associated Protein 1A) ELISA Kit
  • Rat MCP1(Monocyte Chemotactic Protein 1) ELISA Kit
  • Rat MEP1a(Meprin A Alpha) ELISA Kit
  • Rat MT1(Metallothionein 1) ELISA Kit
  • Rat MUC5B(Mucin 5 Subtype B) ELISA Kit
  • Rat NFkB(Nuclear Factor Kappa B) ELISA Kit
  • Rat NGAL(Neutrophil Gelatinase Associated Lipocalin) ELISA Kit
  • Rat NOX4(Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4) ELISA Kit
  • Rat NR3C1(Nuclear Receptor Subfamily 3, Group C, Member 1) ELISA Kit
  • Rat NRG1(Neuregulin 1) ELISA Kit
  • Rat Ntn1(Netrin 1) ELISA Kit
  • Rat PDGFB(Platelet Derived Growth Factor Subunit B) ELISA Kit
  • Rat PIICP(Procollagen II C-Terminal Propeptide) ELISA Kit
  • Rat PIIINP(Procollagen III N-Terminal Propeptide) ELISA Kit
  • Rat PKCa(Protein Kinase C Alpha) ELISA Kit
  • Rat PKCz(Protein Kinase C Zeta) ELISA Kit
  • Rat Plg(Plasminogen) ELISA Kit
  • Rat PP(Pancreatic Polypeptide) ELISA Kit
  • Rat PR3(Proteinase 3) ELISA Kit
  • Rat PRKAb1(Protein Kinase, AMP Activated Beta 1) ELISA Kit
  • Rat PTHrP(Parathyroid Hormone Related Protein) ELISA Kit
  • Rat SDH(Sorbitol Dehydrogenase) ELISA Kit
  • Rat SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit
  • Rat SPA2(Surfactant Associated Protein A2) ELISA Kit
  • Rat SPD(Surfactant Associated Protein D) ELISA Kit
  • Rat SYP(Synaptophysin) ELISA Kit
  • Rat TARC(Thymus Activation Regulated Chemokine) ELISA Kit
  • Rat TFF3(Trefoil Factor 3, Intestinal) ELISA Kit
  • Rat TGFbR1(Transforming Growth Factor Beta Receptor I) ELISA Kit
  • Rat TNC(Tenascin C) ELISA Kit
  • Rat TrxR1(Thioredoxin Reductase 1) ELISA Kit
  • Rat UCN1(Urocortin 1) ELISA Kit
  • Rat VEGFR2(Vascular Endothelial Growth Factor Receptor 2) ELISA Kit
  • Rat vWF(Von Willebrand Factor) ELISA Kit
  • Sheep TIMP1(Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit
  • Simian Immunodeficiency Virus SIV

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