First Report of Barley virus G in the United States and California
Barley (Hordeum vulgare) is a helpful annual cereal crop grown broadly all through the United States and the world. The majority of barley grown commercially in California and all through the U.S. is used for livestock feed, with the the rest getting used by the malting trade and, to a lesser extent, direct meals consumption; additionally it is typically employed as a canopy crop (Lazicki et al. 2016).
Yellow dwarf viruses (YDVs), in the household Luteoviridae, that infect barley and different cereal crops are frequent and broadly distributed all through California and the U.S. (Griesbach et al. 1989; Seabloom et al. 2009). In April 2018, 5 barley samples exhibiting typical signs of YDV an infection (primarily yellowing of leaf margins and suggestions) collected from fields in Yolo county planted with cultivar Butta 12 , have been examined for viruses.
Total RNA was extracted from leaf tissue utilizing Trizol reagent, in keeping with the producer’s protocol. RNA was used as template in a multiplexed RT-PCR assay designed for the generic detection of barley and cereal infecting YDVs, utilizing the protocol established by Malmstrom and Shu (2004). A 372 basepair amplicon indicative of Polerovirus an infection was amplified from two of the samples and sequenced (Quintara Biosciences), and the ensuing information analyzed through a BLASTn search.
No additional testing or work was finished with the three samples that examined unfavourable. Not unexpectedly, the high outcome returned for one among the constructive samples was Cereal yellow dwarf virus-RPV (CYDV-RPV; 98% id), a virus frequent to cereals in California and the U.S.. Unexpectedly, nevertheless, the high outcome returned for the different pattern was Barley virus G (BVG), sharing 98.43% id with the Uiseong BVG isolate (GenBank accession LC259081).
To additional affirm the presence of BVG in the pattern, the full-length viral genome was amplified utilizing two-step RT-PCR with primers concentrating on the excessive 5′ and 3′ ends of the viral genome, utilizing the PrimeScript RT and PrimeSTAR GXL DNA Polymerase kits (Takara Bio), cloned into the binary vector pJL89 and a BLAST search of the ensuing 5621 nucleotide full-length sequence (100% question protection) as soon as once more returned outcomes exhibiting the YDV to be BVG.
The full-length sequence was deposited into GenBank (MW853785). Nucleotide sequence comparisons confirmed that the CA BVG isolate shares 96.62%, 96.57%, and 96.02% id with the sequences of the BVG-Gimje, BVG-Uiseong (LC259081), and BVG-Aus8 (LC500836) isolates, respectively. To our data, that is the first report of barley virus G in California and in the United states.
Currently the prevalence, host vary and mode and timing of introduction of BVG in California and the U.S. are unknown; its influence on cereal manufacturing and yield in any location in which it has been recognized to this point can also be unknown and could warrant additional investigation.
Prevalence, Characterization, and Antimicrobial Susceptibility of Listeria monocytogenes from Raw Beef and Slaughterhouse Environments in Korea
The prevalence of Listeria monocytogenes in uncooked beef and in slaughterhouse environments was investigated from April 2019 to February 2020. Three hundred uncooked beef samples have been bought from 50 retailers and 10 eating places (5 samples per supply).
One hundred and thirty-four samples from slaughterhouse environments have been collected by swabbing (10 × 10 cm) the surfaces, gloves, splitting noticed, and drains. L. monocytogenes was detected and recognized in keeping with the technique described in ISO 11290-1, and confirmed by 16S rRNA sequencing. L. monocytogenes was detected in uncooked beef, gloves used in carcass splitting (6/21, 28.6%), the splitting noticed, and the drain zone (1/15, 6.7%).
All isolates have been serotype 1/2a or 1/2c, primarily based on screening utilizing multiplex PCR-based serogrouping assay and serotyping equipment for O-H antigens. Pulsed-field gel electrophoresis (PFGE) following ApaI digestion of eight PFGE pulsotypes and 4 PFGE teams have been recognized. Biofilm formation evaluation utilizing Crystal Violet staining revealed the highest biofilm formation in pressure LM-16, adopted by D190613.
REAGENT BOTTLE, 1L, PP |
1500P-1L |
CORNING |
10/pk |
EUR 100 |
Description: Reusable Plastics; Resusable Bottles |
BEAKER,1L,PLASTIC,LOW FORM,PP,6/6 |
1000P-1L |
CORNING |
6/pk |
EUR 63 |
Description: Reusable Plastics; Resuable Beakers |
BEAKER,1L,PLASTIC,LOW FORM,PFA,1/6 |
1003P-1L |
CORNING |
1/pk |
EUR 653 |
Description: Reusable Plastics; Resuable Beakers |
BEAKER,1L,WITH HANDLE AND SPOUT,PP,12/12 |
1015P-1L |
CORNING |
12/pk |
EUR 116 |
Description: Reusable Plastics; Resuable Beakers |
CYLINDER,GRAD,1L,PLASTIC,SINGLE METRIC SCALE,PP,TC W/FUNNEL,1/6 |
3022P-1L |
CORNING |
1/pk |
EUR 161 |
Description: Reusable Plastics; Reusable Cylinders |
VOL FLASK,1L,PLASTIC,CLASS A,PMP,24/29 TAPERED PP STOPPER,1/3 |
5640P-1L |
CORNING |
1/pk |
EUR 133 |
Description: Reusable Plastics; Reusable Volumetric Flasks |
VOL FLASK,1L,PLASTIC,CLASS B,PP,24/29 TAPERED PP STOPPER,1/3 |
5641P-1L |
CORNING |
1/pk |
EUR 121 |
Description: Reusable Plastics; Reusable Volumetric Flasks |
PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
PIN400A-KIT |
SBI |
1 Kit |
EUR 2798 |
|
Chymotrypsin for Sequencing grade |
C4001-010 |
GenDepot |
4x25ug |
EUR 313 |
Chymotrypsin for Sequencing grade |
C4001-100 |
GenDepot |
100ug |
EUR 286 |
sequencing unit 33x45 cm |
ESEQ1100-SYS |
Consort |
ea |
EUR 1535 |
Trypin for Mass & Sequencing |
T9600-025 |
GenDepot |
25ug |
EUR 145 |
Trypin for Mass & Sequencing |
T9600-100 |
GenDepot |
100ug |
EUR 219 |
Trypin for Mass & Sequencing |
T9600-112 |
GenDepot |
12x100ug |
EUR 1657 |
Trypin for Mass & Sequencing |
T9600-400 |
GenDepot |
4x100ug |
EUR 651 |
PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
PIN300A-KIT |
SBI |
1 Kit |
EUR 2798 |
|
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
PIN410A-KIT |
SBI |
1 Kit |
EUR 4335 |
|
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
PIN412A-KIT |
SBI |
1 Kit |
EUR 4335 |
|
DNA Library Prep Kit for IIlumina Sequencing |
K1475-12 |
Biovision |
12 Rxns |
EUR 480 |
PLASTIC CLAMP, 1L |
480152 |
CORNING |
1/pk |
EUR 73 |
Description: Lab Equipment; Constant Temperature Equipment |
Beaker Brushes, 1L |
BR007-1K |
Bio Basic |
1 UNIT |
EUR 53.48 |
|
SLINGSHOT-1L Antibody |
abx224128-100ug |
Abbexa |
100 ug |
EUR 411 |
|
SLINGSHOT-1L Antibody |
abx016133-100ug |
Abbexa |
100 ug |
EUR 411 |
|
SLINGSHOT-1L Antibody |
48407-100ul |
SAB |
100ul |
EUR 333 |
SLINGSHOT-1L Antibody |
48407-50ul |
SAB |
50ul |
EUR 239 |
Dermcidin-1L (human) |
H-7316.0500 |
Bachem |
0.5mg |
EUR 177 |
Description: Sum Formula: C210H359N57O71; CAS# [478898-18-9] net |
Dermcidin-1L (human) |
H-7316.1000 |
Bachem |
1.0mg |
EUR 294 |
Description: Sum Formula: C210H359N57O71; CAS# [478898-18-9] net |
PCR Clean Up for DNA Sequencing |
BT5100 |
Bio Basic |
100preps |
EUR 95.68 |
|
PCR Clean Up for DNA Sequencing |
BT5101 |
Bio Basic |
1000Preps, 1000prep |
EUR 461.08 |
|
Frit Kit |
FRIT-KIT |
Next Advance |
1each |
EUR 124 |
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
Column Packing Kit |
PACK-KIT |
Next Advance |
1pack |
EUR 1035 |
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
CELL EXPANSION BAG, 1L |
91-200-85 |
CORNING |
1/pk |
EUR 112 |
Description: Flexible Containers; Bioprocess Bags and Parts |
InstantBlue 1L (Instant Blue) |
ISB1L |
Expedeon |
1L bottle |
EUR 165 |
|
SLINGSHOT-1L Conjugated Antibody |
C48407 |
SAB |
100ul |
EUR 397 |
PCR Mycoplasma Detection Kit |
M034-Kit |
TOKU-E |
Kit |
EUR 266 |
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
CAS400A-KIT |
SBI |
1 kit (10 rxn) |
EUR 1110 |
|
Human Protein phosphatase 1L, PPM1L ELISA KIT |
ELI-45425h |
Lifescience Market |
96 Tests |
EUR 824 |
Bovine Protein phosphatase 1L, PPM1L ELISA KIT |
ELI-36194b |
Lifescience Market |
96 Tests |
EUR 928 |
Mouse Protein phosphatase 1L, Ppm1l ELISA KIT |
ELI-36546m |
Lifescience Market |
96 Tests |
EUR 865 |
ELISA kit for Human Metallothionein-1L (MT1L) |
KTE61511-48T |
Abbkine |
48T |
EUR 332 |
|
Description: Quantitative sandwich ELISA for measuring Human Metallothionein-1L (MT1L) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Metallothionein-1L (MT1L) |
KTE61511-5platesof96wells |
Abbkine |
5 plates of 96 wells |
EUR 2115 |
|
Description: Quantitative sandwich ELISA for measuring Human Metallothionein-1L (MT1L) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
ELISA kit for Human Metallothionein-1L (MT1L) |
KTE61511-96T |
Abbkine |
96T |
EUR 539 |
|
Description: Quantitative sandwich ELISA for measuring Human Metallothionein-1L (MT1L) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids. |
CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV100PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV105PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV120PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV125PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS700A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS720A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
CAS740A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
CAS510A-KIT |
SBI |
1 Kit |
EUR 805 |
|
Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV200PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV205PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV220PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
CASLV225PA-KIT |
SBI |
1 Kit |
EUR 1132 |
|
COLLECTION CONTAINER 1L, EVA FILM |
91-200-01 |
CORNING |
1/pk |
EUR 80 |
Description: Flexible Containers; Bioprocess Bags and Parts |
COLLECTION CONTAINER 1L, EVA FILM |
91-200-42 |
CORNING |
1/pk |
EUR 73 |
Description: Flexible Containers; Bioprocess Bags and Parts |
ICE PAN, MINI, 1L GREEN |
432115 |
CORNING |
1/pk |
EUR 93 |
Description: BioCision; BioCision TruCool |
ICE PAN, MINI, 1L BLUE |
432116 |
CORNING |
1/pk |
EUR 93 |
Description: BioCision; BioCision TruCool |
ICE PAN, MINI, 1L RED |
432117 |
CORNING |
1/pk |
EUR 93 |
Description: BioCision; BioCision TruCool |
ICE PAN, MINI, 1L ORANGE |
432118 |
CORNING |
1/pk |
EUR 93 |
Description: BioCision; BioCision TruCool |
ICE PAN, MINI, 1L PINK |
432120 |
CORNING |
1/pk |
EUR 93 |
Description: BioCision; BioCision TruCool |
ICE PAN, MINI, 1L PURPLE |
432121 |
CORNING |
1/pk |
EUR 93 |
Description: BioCision; BioCision TruCool |
CLAMP FOR 1L ERLENMYER FLASK |
480115 |
CORNING |
1/pk |
EUR 63 |
Description: Lab Equipment; Shakers & Mixers |
Recombinant human Protein phosphatase 1L |
P1206 |
FN Test |
100ug |
Ask for price |
|
Description: Recombinant protein for human Protein phosphatase 1L |
Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
CAS750A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
CAS770A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
CAS790A-KIT |
SBI |
10 rxn |
EUR 1132 |
|
Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
CAS9LIG-KIT |
SBI |
1 Kit |
EUR 153 |
|
PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
PIN320A-KIT |
SBI |
1 Kit |
EUR 4941 |
|
PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
PIN340iPS-KIT |
SBI |
1 Kit |
EUR 4941 |
|
Human Retinoic Acid Early Transcript 1L ELISA kit |
E01R0358-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Human Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Retinoic Acid Early Transcript 1L ELISA kit |
E01R0358-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Human Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Human Retinoic Acid Early Transcript 1L ELISA kit |
E01R0358-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Human Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Retinoic Acid Early Transcript 1L ELISA kit |
E03R0358-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Mouse Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Retinoic Acid Early Transcript 1L ELISA kit |
E03R0358-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Mouse Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Mouse Retinoic Acid Early Transcript 1L ELISA kit |
E03R0358-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Mouse Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Retinoic Acid Early Transcript 1L ELISA kit |
E02R0358-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Rat Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Retinoic Acid Early Transcript 1L ELISA kit |
E02R0358-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Rat Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Rat Retinoic Acid Early Transcript 1L ELISA kit |
E02R0358-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Rat Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Retinoic Acid Early Transcript 1L ELISA kit |
E08R0358-192T |
BlueGene |
192 tests |
EUR 1270 |
|
Description: A competitive ELISA for quantitative measurement of Canine Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Retinoic Acid Early Transcript 1L ELISA kit |
E08R0358-48 |
BlueGene |
1 plate of 48 wells |
EUR 520 |
|
Description: A competitive ELISA for quantitative measurement of Canine Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Dog Retinoic Acid Early Transcript 1L ELISA kit |
E08R0358-96 |
BlueGene |
1 plate of 96 wells |
EUR 685 |
|
Description: A competitive ELISA for quantitative measurement of Canine Retinoic Acid Early Transcript 1L in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
Although L. monocytogenes isolates have been inclined to most antimicrobials, some resistance to penicillin (8/15, 53.3%) and tetracycline (2/15, 13.3%) was noticed. These outcomes recommend that LM-16 has been repeatedly current in biofilms in the slaughterhouse environments since 2006. Our examine signifies that L. monocytogenes contamination in uncooked beef may constantly happen throughout beef processing in slaughterhouse environments by contact with gloves, splitting saws, and drains.