Diabetic nephropathy (DN) critically impacts the individuals’s life and well being in China. This examine aimed to research the impact of circRNA circ-ITCH on bettering DN by regulating the miR-33a-5p/SIRT6 axis and the potential mechanism of motion. High glucose (HG)-induced rat mesangial cells (RMCs) have been used to simulate the DN in vitro.
Reverse transcription-quantitative PCR (RT-qPCR) and western blot evaluation have been carried out to detect the gene or protein expression. Cell Counting Kit-8 (CCK-8) and wound therapeutic assays have been carried out to estimate the cell viability and migration functionality. Immunofluorescence and enzyme-linked immunosorbent assay (ELISA) have been used to detect the α-Smooth Muscle Actin (α-SMA) expression and ranges of inflammatory components.
The potential associations between circ-ITCH and miR-33a-5p, miR-33a-5p and SIRT6 in RMCs have been measured through dual-luciferase reporter assay. Streptozotocin (STZ) was used to induce the diabetic mice. Blood glucose and serum insulin of mice have been decided by corresponding kits, and blood urea nitrogen (BUN) and serum creatinine (SCr) have been measured utilizing an automated biochemical analyzer.
Hematoxylin and eosin (H&E) staining and periodic acid-Schiff (PAS) staining have been utilized to look at the diploma of pathological damage and fibrosis of renal tissues. The outcomes of the current examine revealed that circ-ITCH expression was clearly decreased in HG-induced RMCs. In addition, circ-ITCH overexpression inhibited the viability, migration, fibrosis and inflammatory response of HG-induced RMCs.
Further experiments confirmed that miR-33a-5p could also be a direct goal of circ-ITCH and SIRT6 could also be a direct goal of miR-33a-5p. Notably, the miR-33a-5p mimic or shRNA-SIRT6 have been found to reverse the inhibitory results of circ-ITCH on the proliferation, migration, fibrosis and inflammatory response of HG-induced RMCs. Furthermore, circ-ITCH overexpression ameliorated renal inflammation and fibrosis in STZ-induced diabetic mice.
First Report of Barley virus G in the United States and California
Barley (Hordeum vulgare) is a helpful annual cereal crop grown broadly all through the United States and the world. The majority of barley grown commercially in California and all through the U.S. is used for livestock feed, with the the rest getting used by the malting trade and, to a lesser extent, direct meals consumption; additionally it is typically employed as a canopy crop (Lazicki et al. 2016).
Yellow dwarf viruses (YDVs), in the household Luteoviridae, that infect barley and different cereal crops are frequent and broadly distributed all through California and the U.S. (Griesbach et al. 1989; Seabloom et al. 2009). In April 2018, 5 barley samples exhibiting typical signs of YDV an infection (primarily yellowing of leaf margins and suggestions) collected from fields in Yolo county planted with cultivar Butta 12 , have been examined for viruses.
Total RNA was extracted from leaf tissue utilizing Trizol reagent, in keeping with the producer’s protocol. RNA was used as template in a multiplexed RT-PCR assay designed for the generic detection of barley and cereal infecting YDVs, utilizing the protocol established by Malmstrom and Shu (2004). A 372 basepair amplicon indicative of Polerovirus an infection was amplified from two of the samples and sequenced (Quintara Biosciences), and the ensuing information analyzed through a BLASTn search.
No additional testing or work was finished with the three samples that examined unfavourable. Not unexpectedly, the high outcome returned for one among the constructive samples was Cereal yellow dwarf virus-RPV (CYDV-RPV; 98% id), a virus frequent to cereals in California and the U.S.. Unexpectedly, nevertheless, the high outcome returned for the different pattern was Barley virus G (BVG), sharing 98.43% id with the Uiseong BVG isolate (GenBank accession LC259081).
To additional affirm the presence of BVG in the pattern, the full-length viral genome was amplified utilizing two-step RT-PCR with primers concentrating on the excessive 5′ and 3′ ends of the viral genome, utilizing the PrimeScript RT and PrimeSTAR GXL DNA Polymerase kits (Takara Bio), cloned into the binary vector pJL89 and a BLAST search of the ensuing 5621 nucleotide full-length sequence (100% question protection) as soon as once more returned outcomes exhibiting the YDV to be BVG.
The full-length sequence was deposited into GenBank (MW853785). Nucleotide sequence comparisons confirmed that the CA BVG isolate shares 96.62%, 96.57%, and 96.02% id with the sequences of the BVG-Gimje, BVG-Uiseong (LC259081), and BVG-Aus8 (LC500836) isolates, respectively. To our data, that is the first report of barley virus G in California and in the United states.
Currently the prevalence, host vary and mode and timing of introduction of BVG in California and the U.S. are unknown; its influence on cereal manufacturing and yield in any location in which it has been recognized to this point can also be unknown and could warrant additional investigation.
Prevalence, Characterization, and Antimicrobial Susceptibility of Listeria monocytogenes from Raw Beef and Slaughterhouse Environments in Korea
The prevalence of Listeria monocytogenes in uncooked beef and in slaughterhouse environments was investigated from April 2019 to February 2020. Three hundred uncooked beef samples have been bought from 50 retailers and 10 eating places (5 samples per supply).
One hundred and thirty-four samples from slaughterhouse environments have been collected by swabbing (10 × 10 cm) the surfaces, gloves, splitting noticed, and drains. L. monocytogenes was detected and recognized in keeping with the technique described in ISO 11290-1, and confirmed by 16S rRNA sequencing. L. monocytogenes was detected in uncooked beef, gloves used in carcass splitting (6/21, 28.6%), the splitting noticed, and the drain zone (1/15, 6.7%).
All isolates have been serotype 1/2a or 1/2c, primarily based on screening utilizing multiplex PCR-based serogrouping assay and serotyping equipment for O-H antigens. Pulsed-field gel electrophoresis (PFGE) following ApaI digestion of eight PFGE pulsotypes and 4 PFGE teams have been recognized. Biofilm formation evaluation utilizing Crystal Violet staining revealed the highest biofilm formation in pressure LM-16, adopted by D190613.
UreaGel 29:1 System 1L Kit |
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| NAT1128 | Scientific Laboratory Supplies | EACH | 127.68 EUR |
Filtration System 47mm Complete Assembly |
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| 5809/KIT | Scientific Laboratory Supplies | EACH | 427.69 EUR |
Corning Reagent Bottle 1L PP |
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| 1500P-1L | Scientific Laboratory Supplies | PK10 | 118.56 EUR |
BEAKER,1L,PLASTIC,LOW FORM,PP,6/6 |
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| 1000P-1L | CORNING | 6/pk | 75.6 EUR |
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Description: Reusable Plastics; Resuable Beakers |
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BEAKER,1L,PLASTIC,LOW FORM,PFA,1/6 |
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| 1003P-1L | CORNING | 1/pk | 783.6 EUR |
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Description: Reusable Plastics; Resuable Beakers |
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BEAKER,1L,WITH HANDLE AND SPOUT,PP,12/12 |
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| 1015P-1L | CORNING | 12/pk | 139.2 EUR |
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Description: Reusable Plastics; Resuable Beakers |
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Diss Media Conc Hydrochloric Acid 0.01N (1L to 40L) |
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| DBC10-1L | Scientific Laboratory Supplies | PK6 | 449.16 EUR |
Diss Media Conc Hydrochloric Acid 0.1N (1L to 40L) |
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| DBC11-1L | Scientific Laboratory Supplies | PK6 | 449.16 EUR |
Diss Media Conc K Phosph pH 6.0 (1L to 40L) |
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| DBC02-1L | Scientific Laboratory Supplies | PK6 | 449.16 EUR |
Diss Media Conc K Phosph pH 7.2 (1L to 40L) |
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| DBC04-1L | Scientific Laboratory Supplies | PK6 | 449.16 EUR |
Diss Media Conc K Phosph pH 7.5 (1L to 40L) |
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| DBC05-1L | Scientific Laboratory Supplies | PK6 | 449.16 EUR |
Diss Media Conc Na Lauryl Sulphate 0.50% (1L to 20L) |
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| DBC07-1L | Scientific Laboratory Supplies | 1L | 82.08 EUR |
Diss Media Conc K Phosph pH 8.0 (1L to 40L) |
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| DBC42-1L | Scientific Laboratory Supplies | PK6 | 449.16 EUR |
SequaGel MD SSCP Kit |
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| NAT1170 | Scientific Laboratory Supplies | 1KIT | 217.74 EUR |
SequaGel MD Heteroduplex Kit |
|||
| NAT1172 | Scientific Laboratory Supplies | EACH | 231.42 EUR |
Diss Media Conc K Phosph pH 4.5 (1L of to 40L) |
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| DBC40-1L | Scientific Laboratory Supplies | PK6 | 449.16 EUR |
Diss Media Conc K Phosph pH 7.1 (1L of to 40L) |
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| DBC41-1L | Scientific Laboratory Supplies | PK6 | 449.16 EUR |
Kinesis Filtration System 300ml/1L; replacement Filter Flask |
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| FIL1005 | Scientific Laboratory Supplies | EACH | 88.92 EUR |
EpiNext Bisulfite Sequencing Kit (Illumina) |
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| P-1056-12 | EpiGentek | 12 Reactions | 625.9 EUR |
EpiNext Bisulfite Sequencing Kit (Illumina) |
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| P-1056-24 | EpiGentek | 24 Reactions | 1225.4 EUR |
EpiNext Bisulfite Sequencing Kit (Illumina) |
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| P-1056 | EpiGentek |
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CYLINDER,GRAD,1L,PLASTIC,SINGLE METRIC SCALE,PP,TC W/FUNNEL,1/6 |
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| 3022P-1L | CORNING | 1/pk | 193.2 EUR |
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Description: Reusable Plastics; Reusable Cylinders |
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100ML SequaGel Buffer |
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| NAT1140 | Scientific Laboratory Supplies | 100ML | 24.97 EUR |
450ML SequaGel XR |
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| NAT1160 | Scientific Laboratory Supplies | 450ML | 75.24 EUR |
PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN400A-KIT | SBI | 1 Kit | 3357.6 EUR |
VOL FLASK,1L,PLASTIC,CLASS A,PMP,24/29 TAPERED PP STOPPER,1/3 |
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| 5640P-1L | CORNING | 1/pk | 159.6 EUR |
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Description: Reusable Plastics; Reusable Volumetric Flasks |
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VOL FLASK,1L,PLASTIC,CLASS B,PP,24/29 TAPERED PP STOPPER,1/3 |
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| 5641P-1L | CORNING | 1/pk | 145.2 EUR |
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Description: Reusable Plastics; Reusable Volumetric Flasks |
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PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN300A-KIT | SBI | 1 Kit | 3357.6 EUR |
Chymotrypsin for Sequencing grade |
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| C4001-010 | GenDepot | 4x25ug | 375.6 EUR |
Chymotrypsin for Sequencing grade |
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| C4001-100 | GenDepot | 100ug | 343.2 EUR |
sequencing unit 33x45 cm |
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| ESEQ1100-SYS | Consort | ea | 1842 EUR |
Trypin for Mass & Sequencing |
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| T9600-025 | GenDepot | 25ug | 174 EUR |
Trypin for Mass & Sequencing |
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| T9600-100 | GenDepot | 100ug | 262.8 EUR |
Trypin for Mass & Sequencing |
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| T9600-112 | GenDepot | 12x100ug | 1988.4 EUR |
Trypin for Mass & Sequencing |
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| T9600-400 | GenDepot | 4x100ug | 781.2 EUR |
SDS-Blue™ - Coomassie based solution for protein staining in SDS-PAGE |
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| 04-GSB | Gentaur Genprice |
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Description: SDS-Blue™ is an innovative patented formula, based on Coomassie blue, that comes in a convenient ready to use format for staining proteins in SDS-PAGE (sodium dodecyl sulphate–polyacrylamide gel electrophoresis). The formulation of SDS-Blue™ provides numerous advantages compared to the classic Coomassie staining or to other similar protein stains. SDS-Blue™ provides higher sensitivity, virtually no background and eliminates the need for destaining of the gel due to its high specificity and affinity to bind to protein only. Not only does SDS-Blue™ yield clear and sharp bands, but it also contains no methanol and acetic acid, making it non-hazardous, safe to handle and friendly to the environment when disposed of. Two other advantages that make SDS-Blue™ the better option is that it is not light sensitive and can be stored at ambient temperature for 24 months. And this provides a considerable convenience, especially to laboratories that need and keep big amount of protein staining solutions – no more jammed refrigerators, you can keep SDS-Blue™ wherever it is most convenient for You! |
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PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN410A-KIT | SBI | 1 Kit | 5202 EUR |
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN412A-KIT | SBI | 1 Kit | 5202 EUR |
450ML SequaGel UreaGel Concentrate |
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| NAT1132 | Scientific Laboratory Supplies | 450ML | 75.24 EUR |
200ML SequaGel UreaGel Buffer |
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| NAT1142 | Scientific Laboratory Supplies | 200ML | 28.5 EUR |
450ML SequaGel UreaGel-6 |
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| NAT1144 | Scientific Laboratory Supplies | 450ML | 63.84 EUR |
450ML SequaGel UreaGel-8 |
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| NAT1148 | Scientific Laboratory Supplies | 450ML | 63.84 EUR |
450ML SequaGel UreaGel Diluent |
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| NAT1152 | Scientific Laboratory Supplies | 450ML | 35.68 EUR |
90ML SequaGel Complete Buffer |
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| NAT1156 | Scientific Laboratory Supplies | 90ML | 23.83 EUR |
100ML SequaGel XR Concentrate |
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| NAT1164 | Scientific Laboratory Supplies | 100ML | 78.66 EUR |
450ML SequaGel XR Concentrate |
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| NAT1166 | Scientific Laboratory Supplies | 450ML | 338.58 EUR |
DNA Library Prep Kit for IIlumina Sequencing |
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| K1475-12 | Biovision | 12 Rxns | 576 EUR |
200ML SequaGel UreaGel Complete Buffer |
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| NAT1158 | Scientific Laboratory Supplies | 200ML | 29.75 EUR |
200ML SequaGel MD Monomer Solution |
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| NAT1168 | Scientific Laboratory Supplies | 200ML | 181.26 EUR |
MagaDye™ 4 Color Sanger Sequencing Terminator Kit |
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| 17068 | AAT Bioquest | 5 nmoles | 2091 EUR |
Frit Kit |
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| FRIT-KIT | Next Advance | 1each | 148.8 EUR |
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Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
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1L Hanna pH 6.86 buffer 1L |
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| HI70061L | Scientific Laboratory Supplies | 1L | 29.64 EUR |
1L HarrisHematoxylin |
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| NAT1338 | Scientific Laboratory Supplies | 1L | 100.32 EUR |
1L Autofluor |
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| NAT1446 | Scientific Laboratory Supplies | 1L | 223.44 EUR |
Sharpsbin 1L |
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| SAF7352 | Scientific Laboratory Supplies | EACH | 3.42 EUR |
Percoll 1L |
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| 17089101 | Scientific Laboratory Supplies | EACH | 425.22 EUR |
PCR Clean Up for DNA Sequencing |
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| BT5100 | Bio Basic | 100preps | 114.82 EUR |
PCR Clean Up for DNA Sequencing |
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| BT5101 | Bio Basic | 1000Preps, 1000prep | 553.3 EUR |
Column Packing Kit |
|||
| PACK-KIT | Next Advance | 1pack | 1242 EUR |
|
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
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Watershed System Kit |
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| M-WS180320-1 | MiTeGen | 1 KIT | 9296 EUR |
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Description: Watershed System Kit |
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PCR Mycoplasma Detection Kit |
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| M034-Kit | TOKU-E | Kit | 319.2 EUR |
1.2ML SequaGel MD SSCP Stop Solution |
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| NAT1174 | Scientific Laboratory Supplies | 1.2ML | 29.75 EUR |
1L Opticlear Sample Kit II |
|||
| NAT1496 | Scientific Laboratory Supplies | 1L | 175.56 EUR |
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
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| CAS400A-KIT | SBI | 1 kit (10 rxn) | 1332 EUR |
Pesticide Kit Containing All 10 Multi-Compound Mixes |
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| SPXPR-KIT | Scientific Laboratory Supplies | 1ML | 1923.18 EUR |
CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV100PA-KIT | SBI | 1 Kit | 1358.4 EUR |
CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV105PA-KIT | SBI | 1 Kit | 1358.4 EUR |
MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV120PA-KIT | SBI | 1 Kit | 1358.4 EUR |
MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV125PA-KIT | SBI | 1 Kit | 1358.4 EUR |
Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS700A-KIT | SBI | 10 rxn | 1358.4 EUR |
Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS720A-KIT | SBI | 10 rxn | 1358.4 EUR |
Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS740A-KIT | SBI | 10 rxn | 1358.4 EUR |
Beaker Brushes, 1L |
|||
| BR007-1K | Bio Basic | 1 UNIT | 64.18 EUR |
SLINGSHOT-1L Antibody |
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| 48407-100ul | SAB | 100ul | 399.6 EUR |
SLINGSHOT-1L Antibody |
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| 48407-50ul | SAB | 50ul | 286.8 EUR |
SLINGSHOT-1L Antibody |
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| abx016133-100ug | Abbexa | 100 ug | 493.2 EUR |
SLINGSHOT-1L Antibody |
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| abx224128-100ug | Abbexa | 100 ug | 493.2 EUR |
1L-chiro-Inositol |
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| GC7626-100MG | Glentham Life Sciences | 100 mg | 122.4 EUR |
1L-chiro-Inositol |
|||
| GC7626-1G | Glentham Life Sciences | 1 g | 322.8 EUR |
Dermcidin-1L (human) |
|||
| H-7316.0500 | Bachem | 0.5mg | 212.4 EUR |
|
Description: Sum Formula: C210H359N57O71; CAS# [478898-18-9] net |
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Dermcidin-1L (human) |
|||
| H-7316.1000 | Bachem | 1.0mg | 352.8 EUR |
|
Description: Sum Formula: C210H359N57O71; CAS# [478898-18-9] net |
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1L Flask Clamp |
|||
| MIX1332 | Scientific Laboratory Supplies | EACH | 30.78 EUR |
1L Tween 20 |
|||
| NAT1084 | Scientific Laboratory Supplies | 1L | 124.26 EUR |
1L ProtoBlue Safe |
|||
| NAT1108 | Scientific Laboratory Supplies | 1L | 112.86 EUR |
1L ProtoStain Blue |
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| NAT1112 | Scientific Laboratory Supplies | 1L | 118.56 EUR |
1L AcrylaGel 30% |
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| NAT1118 | Scientific Laboratory Supplies | 1L | 133.38 EUR |
1L TBE 10X |
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| NAT1196 | Scientific Laboratory Supplies | 1L | 44 EUR |
1L TBE 5X |
|||
| NAT1200 | Scientific Laboratory Supplies | 1L | 36.82 EUR |
1L Denaturation Solution |
|||
| NAT1232 | Scientific Laboratory Supplies | 1L | 48.79 EUR |
1L Neutralization Solution |
|||
| NAT1234 | Scientific Laboratory Supplies | 1L | 48.79 EUR |
1L ProtoGel (30%) |
|||
| NAT1260 | Scientific Laboratory Supplies | 1L | 135.66 EUR |
1L ProtoGel (40%) |
|||
| NAT1264 | Scientific Laboratory Supplies | 1L | 161.88 EUR |
1L Scotts Tapwater |
|||
| NAT1342 | Scientific Laboratory Supplies | 1L | 34.54 EUR |
1L Oxosol 306 |
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| NAT1382 | Scientific Laboratory Supplies | 1L | 86.64 EUR |
Flask Receiving 1L |
|||
| EVA5012 | Scientific Laboratory Supplies | EACH | 204.06 EUR |
Arsenite 0.05M 1L |
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| SA2005F | Scientific Laboratory Supplies | 1L | 101.46 EUR |
Stainless Container 1L |
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| SAM1111 | Scientific Laboratory Supplies | EACH | 263.34 EUR |
Scoop 1L Polypropylene |
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| SCO1016 | Scientific Laboratory Supplies | EACH | 28.16 EUR |
GL45 Flask - 1L |
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| FLA1006 | Scientific Laboratory Supplies | PK10 | 241.68 EUR |
Flask Filter 1L |
|||
| FLA7724 | Scientific Laboratory Supplies | PK5 | 282.12 EUR |
Beaker 1L SSteel |
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| BEA3328 | Scientific Laboratory Supplies | EACH | 104.88 EUR |
Percoll Plus 1L |
|||
| 17544501 | Scientific Laboratory Supplies | EACH | 641.82 EUR |
Hellmanexcleaning concentrate 1l |
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| CEL2106 | Scientific Laboratory Supplies | EACH | 79.8 EUR |
Dosing Bottle 1L |
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| CLE2020 | Scientific Laboratory Supplies | EACH | 8.16 EUR |
Sepharose 6B 1L |
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| 17011001 | Scientific Laboratory Supplies | EACH | 918.84 EUR |
Sepharose 4B 1L |
|||
| 17012001 | Scientific Laboratory Supplies | EACH | 918.84 EUR |
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
|||
| CAS510A-KIT | SBI | 1 Kit | 966 EUR |
Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV200PA-KIT | SBI | 1 Kit | 1358.4 EUR |
Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV205PA-KIT | SBI | 1 Kit | 1358.4 EUR |
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV220PA-KIT | SBI | 1 Kit | 1358.4 EUR |
Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
|||
| CASLV225PA-KIT | SBI | 1 Kit | 1358.4 EUR |
Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
|||
| CAS750A-KIT | SBI | 10 rxn | 1358.4 EUR |
Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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| CAS770A-KIT | SBI | 10 rxn | 1358.4 EUR |
Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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| CAS790A-KIT | SBI | 10 rxn | 1358.4 EUR |
Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
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| CAS9LIG-KIT | SBI | 1 Kit | 183.6 EUR |
Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL |
|||
| SPXMT-KIT | Scientific Laboratory Supplies | 30ML | 237.12 EUR |
PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
|||
| PIN320A-KIT | SBI | 1 Kit | 5929.2 EUR |
Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL |
|||
| SPXHM-KIT | Scientific Laboratory Supplies | 30ML | 245.1 EUR |
PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
|||
| PIN340iPS-KIT | SBI | 1 Kit | 5929.2 EUR |
Human Metallothionein- 1L, MT1L ELISA KIT |
|||
| ELI-36664h | Lifescience Market | 96 Tests | 988.8 EUR |
Human Metallothionein 1L(MT1L)ELISA Kit |
|||
| QY-E02761 | Qayee Biotechnology | 96T | 433.2 EUR |
ProtoBlock System |
|||
| NAT1004 | Scientific Laboratory Supplies | 1KIT | 71.82 EUR |
New system |
|||
| VS10WPGS2 | Scientific Laboratory Supplies | EACH | 80.94 EUR |
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|||
| GE620A-KIT | SBI | 1 kit | 2558.4 EUR |
AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|||
| GE622A-KIT | SBI | 1 kit | 2558.4 EUR |
AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|||
| GE624A-KIT | SBI | 1 kit | 2558.4 EUR |
BCA Protein Assay Kit |
|||
| AR0146-1L | BosterBio | 1kit | 547.2 EUR |
SLINGSHOT-1L Conjugated Antibody |
|||
| C48407 | SAB | 100ul | 476.4 EUR |
CELL EXPANSION BAG, 1L |
|||
| 91-200-85 | CORNING | 1/pk | 134.4 EUR |
|
Description: Flexible Containers; Bioprocess Bags and Parts |
|||
InstantBlue 1L (Instant Blue) |
|||
| ISB1L | Expedeon | 1L bottle | 198 EUR |
Kartell Jug 1L PP |
|||
| JUG1002 | Scientific Laboratory Supplies | EACH | 9.12 EUR |
Jug StSteel 1L Graduated |
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| JUG2002 | Scientific Laboratory Supplies | EACH | 42.18 EUR |
1L DEPC Treated Water |
|||
| NAT1104 | Scientific Laboratory Supplies | 1L | 69.54 EUR |
Although L. monocytogenes isolates have been inclined to most antimicrobials, some resistance to penicillin (8/15, 53.3%) and tetracycline (2/15, 13.3%) was noticed. These outcomes recommend that LM-16 has been repeatedly current in biofilms in the slaughterhouse environments since 2006. Our examine signifies that L. monocytogenes contamination in uncooked beef may constantly happen throughout beef processing in slaughterhouse environments by contact with gloves, splitting saws, and drains.