Toehold-mediated DNA strand displacement-driven super-fast tripedal DNA walker for ultrasensitive and label-free electrochemical detection of ochratoxin A
DNA walkers, as clever synthetic DNA nanomachines, have been extensively used as environment friendly nucleic acid amplification instruments that the detection sensitivity may be improved by incorporating DNA walkers into DNA biosensors. Nevertheless, because the untimely launch or flameout in a area of regionally exhausted substrate, the strolling effectivity of DNA walkers stays unsatisfactory.
In this work, we design a sensible tripedal DNA walker that’s fashioned by target-initiated catalyzed hairpin meeting (CHA), which may transfer alongside the DNA duplex tracks on electrode pushed by toehold-mediated DNA strand displacement (TMSD) for transduction and amplification of electrochemical alerts.
Emphatically, this versatile tripedal DNA walker is able to strolling freely alongside the tracks with unconstrained strolling vary. Moreover, the design of multi-legged walker can weaken the derailment of leg DNA and shorten the shifting time on electrode, making certain the processive strolling with excessive effectivity.
Additionally, the persistent strolling of tripedal walker is pushed by cascading TMSD, which eliminates the defects of excessive price and instability of enzyme-assisted amplification expertise. Therefore, the tripedal DNA walker-based electrochemical biosensor has monumental potential for the purposes of OTA detection, and divulges a brand new avenue for meals security evaluation and scientific prognosis.