Downregulation of miR‑106b‑3p increases sensitivity to cisplatin in esophageal cancer cells by targeting TGM3

Expression and effect of microRNA-627 in human hypertrophic scar

To examine the expression and impact of microRNA-627 (miR-627) in human hypertrophic scar. The experimental analysis technique was used. From October 2019 to January 2020, hypertrophic scar tissue from 6 sufferers with hypertrophic scar (2 males and four females, aged (34±11) years) and the remaining regular pores and skin tissue from 6 trauma sufferers (Three males and three females, aged (35±13) years) after flap transplantation had been collected.

The above-mentioned 12 sufferers had been admitted to the General Hospital of Northern Theater Command and met the inclusion standards. The mRNA expression of miR-627 was detected by real-time fluorescent quantitative reverse transcription polymerase chain response.

The third to fifth passages of fibroblasts (Fbs) had been remoted from hypertrophic scar tissue and cultured for subsequent experiments after identification. Fbs from hypertrophic scar had been divided into miR-627 adverse management group, miR-627 mimic group, and miR-627 inhibitor group. The corresponding sequences had been transfected respectively. At 0 (instantly), 12, 24, 36, and 48 h after transfection, the cell viability was detected by thiazolyl blue technique; at 24 h after transfection, the apoptosis was detected by stream cytometry; at 24 h after transfection, the protein expression ranges of insulin-like development issue Ⅰ (IGF-Ⅰ), sort Ⅰ collagen, and α easy muscle actin (α-SMA) had been detected by Western blotting.

Two batches of Fbs from hypertrophic scar had been used, one batch was divided into IGF-Ⅰ wild sort+miR-627 adverse management group and IGF-Ⅰ wild sort+miR-627 mimic group, and the opposite batch was divided into IGF-Ⅰ mutant+miR-627 adverse management group and IGF-Ⅰ mutant+miR-627 mimic group. The corresponding sequences had been transfected respectively.

At 48 h after transfection, the expressions of luciferase and renal luciferase had been detected by luciferase reporter gene detection equipment, and the ratio of the 2 was calculated to mirror the exercise of IGF-Ⅰ. Fbs from hypertrophic scar had been divided into miR-627 adverse management group, miR-627 mimic alone group, and miR-627 mimic+IGF-Ⅰ group, and had been transfected with the corresponding sequences respectively.

1L SequaGel XR
NAT1162	National Diagnostics	1L	EUR 138

sequencing unit 33x45 cm
ESEQ1100-SYS	Consort	ea	EUR 1535

Trypin for Mass & Sequencing
T9600-025	GenDepot	25ug	EUR 145

Trypin for Mass & Sequencing
T9600-100	GenDepot	100ug	EUR 219

Trypin for Mass & Sequencing
T9600-112	GenDepot	12x100ug	EUR 1657

Trypin for Mass & Sequencing
T9600-400	GenDepot	4x100ug	EUR 651

Chymotrypsin for Sequencing grade
C4001-010	GenDepot	4x25ug	EUR 313

Chymotrypsin for Sequencing grade
C4001-100	GenDepot	100ug	EUR 286

PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN400A-KIT	SBI	1 Kit	EUR 2798

PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN300A-KIT	SBI	1 Kit	EUR 2798

450ML SequaGel UreaGel Concentrate
NAT1132	National Diagnostics	450ML	EUR 105

1L SequaGel UreaGel Concentrate
NAT1134	National Diagnostics	1L	EUR 134

200ML SequaGel UreaGel Buffer
NAT1142	National Diagnostics	200ML	EUR 70

450ML SequaGel UreaGel-6
NAT1144	National Diagnostics	450ML	EUR 96

1L SequaGel UreaGel-6
NAT1146	National Diagnostics	1L	EUR 126

450ML SequaGel UreaGel-8
NAT1148	National Diagnostics	450ML	EUR 96

1L SequaGel UreaGel-8
NAT1150	National Diagnostics	1L	EUR 126

450ML SequaGel UreaGel Diluent
NAT1152	National Diagnostics	450ML	EUR 75

1L SequaGel UreaGel Diluent
NAT1154	National Diagnostics	1L	EUR 86

90ML SequaGel Complete Buffer
NAT1156	National Diagnostics	90ML	EUR 66

100ML SequaGel XR Concentrate
NAT1164	National Diagnostics	100ML	EUR 107

450ML SequaGel XR Concentrate
NAT1166	National Diagnostics	450ML	EUR 301

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN410A-KIT	SBI	1 Kit	EUR 4335

PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PIN412A-KIT	SBI	1 Kit	EUR 4335

DNA Library Prep Kit for IIlumina Sequencing
K1475-12	Biovision	12 Rxns	EUR 480

200ML SequaGel UreaGel Complete Buffer
NAT1158	National Diagnostics	200ML	EUR 71

200ML SequaGel MD Monomer Solution
NAT1168	National Diagnostics	200ML	EUR 184

PCR Clean Up for DNA Sequencing
BT5100	Bio Basic	100preps	EUR 95.68

PCR Clean Up for DNA Sequencing
BT5101	Bio Basic	1000Preps, 1000prep	EUR 461.08

1.2ML SequaGel MD SSCP Stop Solution
NAT1174	National Diagnostics	1.2ML	EUR 71

Frit Kit
FRIT-KIT	Next Advance	1each	EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit
PACK-KIT	Next Advance	1pack	EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit
M034-Kit	TOKU-E	Kit	EUR 266

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS400A-KIT	SBI	1 kit (10 rxn)	EUR 1110

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV100PA-KIT	SBI	1 Kit	EUR 1132

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV105PA-KIT	SBI	1 Kit	EUR 1132

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV120PA-KIT	SBI	1 Kit	EUR 1132

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
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Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS700A-KIT	SBI	10 rxn	EUR 1132

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
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Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
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T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)
CAS510A-KIT	SBI	1 Kit	EUR 805

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV200PA-KIT	SBI	1 Kit	EUR 1132

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV205PA-KIT	SBI	1 Kit	EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV220PA-KIT	SBI	1 Kit	EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV225PA-KIT	SBI	1 Kit	EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS750A-KIT	SBI	10 rxn	EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS770A-KIT	SBI	10 rxn	EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS790A-KIT	SBI	10 rxn	EUR 1132

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]
CAS9LIG-KIT	SBI	1 Kit	EUR 153

ProtoBlock System
NAT1004	National Diagnostics	1KIT	EUR 102

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN320A-KIT	SBI	1 Kit	EUR 4941

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PIN340iPS-KIT	SBI	1 Kit	EUR 4941

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE620A-KIT	SBI	1 kit	EUR 2132

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE622A-KIT	SBI	1 kit	EUR 2132

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vWF Acty. Kit
ABP-ACT-KIT	Abpcorp	12 x 8 microwells	EUR 428

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data acquisition system
D230	Consort	ea	EUR 1704

CCD cooling system
3600100	Atto	1unit	EUR 4914

Fast Mutagenesis System
20-abx098073	Abbexa	EUR 509.00 EUR 592.00	10 rxns 20 rxns

CometAssay Electrophoresis System
4250-050-ES	Biotechne	1 Kit	EUR 2950.8

Cometslides Rack System
4252-040-02	Biotechne	1 Each	EUR 213.8

photo documentation system
VLBPCX4-20M	Consort	ea	EUR 6352

photo documentation system
VLBPCX4-20MX	Consort	ea	EUR 6774

photo documentation system
VLDP-HOOD	Consort	ea	EUR 5084

photo documentation system
VLDP17-26M	Consort	ea	EUR 8126

photo documentation system
VLDP17-26MX	Consort	ea	EUR 8464

AEC coloring system
CT356	U-CyTech	10-plate	EUR 141

hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9)
CAS620A-KIT	SBI	1 kit	EUR 2152
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression)

PrecisionX Multiplex gRNA Cloning Kit
CAS9-GRNA-KIT	SBI	10 rxn	EUR 445

UreaGel 29:1 System 1L Kit
NAT1128	National Diagnostics	EACH	EUR 143

SBA Clonotyping System-AP Kit (OKSB00002)
OKSB00002	Aviva Systems Biology	1 Kit	EUR 846
Description: Description of target: ;Species reactivity: ;Application: ELISA, ELISPOT;Assay info: ;Sensitivity:

SBA Clonotyping System Kit : HRP (OKSB00003)
OKSB00003	Aviva Systems Biology	1 Kit	EUR 787
Description: Description of target: ;Species reactivity: ;Application: ELISA;Assay info: ;Sensitivity:

mRNAExpress mRNA Synthesis kit (5 reactions)
MR-KIT-1	SBI	5 reactions	EUR 1152

modular system 10x10 cm
EVS1100-MULTI	Consort	ea	EUR 1197

modular system 20x10 cm
EVS1200-MULTI	Consort	ea	EUR 1451

modular system 20x20 cm
EVS1300-MULTI	Consort	ea	EUR 1958

vertical system 10x10 cm
EVS3100-SYS	Consort	ea	EUR 775

vertical system 20x10 cm
EVS3200-SYS	Consort	ea	EUR 1451

vertical system 20x20 cm
EVS3300-SYS	Consort	ea	EUR 1704

mini system 7x8 cm
EHS3100-SYS	Consort	ea	EUR 437

mini system 8x10 cm
EHS1050-SYS	Consort	ea	EUR 352

Fast MultiSite Mutagenesis System
abx098861-10rxns	Abbexa	10 rxns	EUR 690

myGel Mini Electrophoresis System
BM0286	GenDepot	System	EUR 618

96-Well Vacuum System
SD5011	Bio Basic	1 UNIT	EUR 324.05

amfiRivert Reverse Transcription System
R5200-050	GenDepot	50 rxns	EUR 388

EasyDip Slide Staining System
M900-12AS-ASSORTED	ScyTek Laboratories	1 Case(s)	EUR 176

cfMAX cfDNA Isolation System
CFMAX100A-1	SBI	1 Kit	EUR 479

Episomal iPSC Reprogramming System
SC900A-1	SBI	5 reactions	EUR 968

FractionPREP Cell Fractionation system
55R-1359	Fitzgerald	50 assays	EUR 758
Description: Cell Fractionation system for use in the research laboratory

ExoAb Antibody Kit (CD9, CD63, CD81, Hsp70 antibodies, rabbit anti-human) with goat anti-rabbit HRP secondary antibody
EXOAB-KIT-1	SBI	25 ul each	EUR 627

At 24 h after transfection, the protein expression ranges of IGF-Ⅰ, sort Ⅰ collagen, and α-SMA had been detected by Western blotting. The quantity of samples in cell experiment was 3. Data had been statistically analyzed with evaluation of variance for factorial design, one-way evaluation of variance, impartial pattern t check, and chi-square check.