Esophageal cancer (EC) is one of essentially the most malignant and deadly digestive‑associated tumors worldwide. However, acquired drug resistance is a significant impediment regarding anticancer chemotherapy. An growing quantity of research have reported that microRNAs (miRNAs/miRs) are implicated in regulating the sensitivity of drug resistance in esophageal squamous cell carcinoma (ESCC).
The intention of the current examine was to examine the position of miR‑106b‑3p in the sensitivity of cisplatin for ESCC. Initially, reverse transcription‑quantitative polymerase chain response (RT‑qPCR) was carried out to analyze miR‑106b‑3p and protein‑glutamine γ‑glutamyltransferase E (TGM3) expression ranges in ESCC and non‑tumor adjoining tissues.
By utilizing bioinformatics software program TargetScan, TGM3 was predicted to be a possible downstream goal of miR‑106‑3p. Following verification that TGM3 was a downstream goal of miR‑106b‑3p by the twin‑luciferase reporter assay, the results of miR‑106b‑3p transfection on KYSE30 cell viability and apoptosis following therapy with cisplatin had been confirmed utilizing Cell Counting Kit‑eight and stream cytometry assays, respectively.
The outcomes revealed that miR‑106b‑3p ranges had been upregulated, whereas TMG3 ranges had been downregulated in ESCC tissues. Dual‑luciferase reporter assays confirmed that miR‑106b‑3p negatively regulated TGM3 expression by binding to its 3’UTR sequence. It was additionally proven that inhibition of miR‑106b‑3p might improve the anti‑proliferative results, whereas selling the apoptotic results of cisplatin in the KYSE30 cell line by targeting TGM3. In conclusion, the current examine demonstrated that downregulation of miR‑106b‑3p could improve the sensitivity of KYSE30 cell to cisplatin by targeting TGM3.
Ketogenic food plan alleviates colitis by discount of colonic group Three innate lymphoid cells by way of altering intestine microbiome
Accumulating proof means that ketogenic diets (KDs) mediate the rise of circulating ketone our bodies and exert a possible anti-inflammatory impact; nonetheless, the results of this distinctive food plan on colitis stay unknown. We carried out a sequence of systematic research utilizing a dextran sulfate sodium (DSS) animal mannequin of inflammatory colitis.
Animals had been fed with a KD, low-carbohydrate food plan (LCD), or regular food plan (ND). Germ-free mice had been utilized in validation experiments. Colon tissues had been analyzed by transcriptome sequencing, RT2 profiler PCR array, histopathology, and immunofluorescence. Serum samples had been analyzed by metabolic assay equipment.
Fecal samples had been analyzed by 16S rRNA gene sequencing, liquid chromatography-mass spectrometry and gasoline chromatography-mass spectrometry. We noticed that KD alleviated colitis by altering the intestine microbiota and metabolites in a way distinct from LCD. Quantitative food plan experiments confirmed the distinctive affect of KD relative to LCD with a reproducible improve in Akkermansia, whereas the other was noticed for Escherichia/Shigella.
After colitis induction, the KD protected intestinal barrier operate, and diminished the manufacturing of RORγt+CD3– group Three innate lymphoid cells (ILC3s) and associated inflammatory cytokines (IL-17α, IL-18, IL-22, Ccl4). Finally, fecal microbiota transplantation into germ-free mice revealed that the KD- mediated colitis inhibition and ILC3 regulation had been depending on the modification of intestine microbiota.
Taken collectively, our examine presents a world view of microbiome-metabolomics modifications that happen throughout KD colitis therapy, and identifies the regulation of intestine microbiome and ILC3s as novel targets involving in IBD dietary remedy.
Expression and effect of microRNA-627 in human hypertrophic scar
To examine the expression and impact of microRNA-627 (miR-627) in human hypertrophic scar. The experimental analysis technique was used. From October 2019 to January 2020, hypertrophic scar tissue from 6 sufferers with hypertrophic scar (2 males and four females, aged (34±11) years) and the remaining regular pores and skin tissue from 6 trauma sufferers (Three males and three females, aged (35±13) years) after flap transplantation had been collected.
The above-mentioned 12 sufferers had been admitted to the General Hospital of Northern Theater Command and met the inclusion standards. The mRNA expression of miR-627 was detected by real-time fluorescent quantitative reverse transcription polymerase chain response.
The third to fifth passages of fibroblasts (Fbs) had been remoted from hypertrophic scar tissue and cultured for subsequent experiments after identification. Fbs from hypertrophic scar had been divided into miR-627 adverse management group, miR-627 mimic group, and miR-627 inhibitor group. The corresponding sequences had been transfected respectively. At 0 (instantly), 12, 24, 36, and 48 h after transfection, the cell viability was detected by thiazolyl blue technique; at 24 h after transfection, the apoptosis was detected by stream cytometry; at 24 h after transfection, the protein expression ranges of insulin-like development issue Ⅰ (IGF-Ⅰ), sort Ⅰ collagen, and α easy muscle actin (α-SMA) had been detected by Western blotting.
Two batches of Fbs from hypertrophic scar had been used, one batch was divided into IGF-Ⅰ wild sort+miR-627 adverse management group and IGF-Ⅰ wild sort+miR-627 mimic group, and the opposite batch was divided into IGF-Ⅰ mutant+miR-627 adverse management group and IGF-Ⅰ mutant+miR-627 mimic group. The corresponding sequences had been transfected respectively.
At 48 h after transfection, the expressions of luciferase and renal luciferase had been detected by luciferase reporter gene detection equipment, and the ratio of the 2 was calculated to mirror the exercise of IGF-Ⅰ. Fbs from hypertrophic scar had been divided into miR-627 adverse management group, miR-627 mimic alone group, and miR-627 mimic+IGF-Ⅰ group, and had been transfected with the corresponding sequences respectively.
EpiNext Bisulfite Sequencing Kit (Illumina) |
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| P-1056-24 | EpiGentek | 24 Reactions | 1225.4 EUR |
EpiNext Bisulfite Sequencing Kit (Illumina) |
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| P-1056 | EpiGentek |
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100ML SequaGel Buffer |
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| NAT1140 | Scientific Laboratory Supplies | 100ML | 24.97 EUR |
450ML SequaGel XR |
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| NAT1160 | Scientific Laboratory Supplies | 450ML | 75.24 EUR |
1L SequaGel XR |
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| NAT1162 | Scientific Laboratory Supplies | 1L | 119.7 EUR |
PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN400A-KIT | SBI | 1 Kit | 3357.6 EUR |
Chymotrypsin for Sequencing grade |
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| C4001-010 | GenDepot | 4x25ug | 375.6 EUR |
Chymotrypsin for Sequencing grade |
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| C4001-100 | GenDepot | 100ug | 343.2 EUR |
sequencing unit 33x45 cm |
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| ESEQ1100-SYS | Consort | ea | 1842 EUR |
Trypin for Mass & Sequencing |
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| T9600-025 | GenDepot | 25ug | 174 EUR |
Trypin for Mass & Sequencing |
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| T9600-100 | GenDepot | 100ug | 262.8 EUR |
Trypin for Mass & Sequencing |
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| T9600-112 | GenDepot | 12x100ug | 1988.4 EUR |
Trypin for Mass & Sequencing |
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| T9600-400 | GenDepot | 4x100ug | 781.2 EUR |
PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN300A-KIT | SBI | 1 Kit | 3357.6 EUR |
450ML SequaGel UreaGel Concentrate |
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| NAT1132 | Scientific Laboratory Supplies | 450ML | 75.24 EUR |
1L SequaGel UreaGel Concentrate |
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| NAT1134 | Scientific Laboratory Supplies | 1L | 109.44 EUR |
200ML SequaGel UreaGel Buffer |
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| NAT1142 | Scientific Laboratory Supplies | 200ML | 28.5 EUR |
450ML SequaGel UreaGel-6 |
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| NAT1144 | Scientific Laboratory Supplies | 450ML | 63.84 EUR |
1L SequaGel UreaGel-6 |
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| NAT1146 | Scientific Laboratory Supplies | 1L | 103.74 EUR |
450ML SequaGel UreaGel-8 |
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| NAT1148 | Scientific Laboratory Supplies | 450ML | 63.84 EUR |
1L SequaGel UreaGel-8 |
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| NAT1150 | Scientific Laboratory Supplies | 1L | 103.74 EUR |
450ML SequaGel UreaGel Diluent |
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| NAT1152 | Scientific Laboratory Supplies | 450ML | 35.68 EUR |
1L SequaGel UreaGel Diluent |
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| NAT1154 | Scientific Laboratory Supplies | 1L | 49.93 EUR |
90ML SequaGel Complete Buffer |
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| NAT1156 | Scientific Laboratory Supplies | 90ML | 23.83 EUR |
100ML SequaGel XR Concentrate |
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| NAT1164 | Scientific Laboratory Supplies | 100ML | 78.66 EUR |
450ML SequaGel XR Concentrate |
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| NAT1166 | Scientific Laboratory Supplies | 450ML | 338.58 EUR |
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN410A-KIT | SBI | 1 Kit | 5202 EUR |
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN412A-KIT | SBI | 1 Kit | 5202 EUR |
DNA Library Prep Kit for IIlumina Sequencing |
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| K1475-12 | Biovision | 12 Rxns | 576 EUR |
200ML SequaGel UreaGel Complete Buffer |
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| NAT1158 | Scientific Laboratory Supplies | 200ML | 29.75 EUR |
200ML SequaGel MD Monomer Solution |
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| NAT1168 | Scientific Laboratory Supplies | 200ML | 181.26 EUR |
MagaDye™ 4 Color Sanger Sequencing Terminator Kit |
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| 17068 | AAT Bioquest | 5 nmoles | 2091 EUR |
PCR Clean Up for DNA Sequencing |
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| BT5100 | Bio Basic | 100preps | 114.82 EUR |
PCR Clean Up for DNA Sequencing |
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| BT5101 | Bio Basic | 1000Preps, 1000prep | 553.3 EUR |
Frit Kit |
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| FRIT-KIT | Next Advance | 1each | 148.8 EUR |
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Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
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Watershed System Kit |
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| M-WS180320-1 | MiTeGen | 1 KIT | 9296 EUR |
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Description: Watershed System Kit |
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Column Packing Kit |
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| PACK-KIT | Next Advance | 1pack | 1242 EUR |
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Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
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PCR Mycoplasma Detection Kit |
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| M034-Kit | TOKU-E | Kit | 319.2 EUR |
1.2ML SequaGel MD SSCP Stop Solution |
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| NAT1174 | Scientific Laboratory Supplies | 1.2ML | 29.75 EUR |
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
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| CAS400A-KIT | SBI | 1 kit (10 rxn) | 1332 EUR |
Pesticide Kit Containing All 10 Multi-Compound Mixes |
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| SPXPR-KIT | Scientific Laboratory Supplies | 1ML | 1923.18 EUR |
Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
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| CAS410A-KIT | SBI | 1 kit (10 rxn) | 798 EUR |
Cas9-EGFP Protein and T7 gRNA SmartNuclease Synthesis Kit |
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| CAS420A-KIT | SBI | 1 kit (10 rxn) | 828 EUR |
CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV100PA-KIT | SBI | 1 Kit | 1358.4 EUR |
CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV105PA-KIT | SBI | 1 Kit | 1358.4 EUR |
MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV120PA-KIT | SBI | 1 Kit | 1358.4 EUR |
MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV125PA-KIT | SBI | 1 Kit | 1358.4 EUR |
Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS700A-KIT | SBI | 10 rxn | 1358.4 EUR |
Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS720A-KIT | SBI | 10 rxn | 1358.4 EUR |
Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS740A-KIT | SBI | 10 rxn | 1358.4 EUR |
T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
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| CAS510A-KIT | SBI | 1 Kit | 966 EUR |
Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV200PA-KIT | SBI | 1 Kit | 1358.4 EUR |
Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV205PA-KIT | SBI | 1 Kit | 1358.4 EUR |
Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV220PA-KIT | SBI | 1 Kit | 1358.4 EUR |
Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV225PA-KIT | SBI | 1 Kit | 1358.4 EUR |
Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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| CAS750A-KIT | SBI | 10 rxn | 1358.4 EUR |
Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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| CAS770A-KIT | SBI | 10 rxn | 1358.4 EUR |
Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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| CAS790A-KIT | SBI | 10 rxn | 1358.4 EUR |
Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
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| CAS9LIG-KIT | SBI | 1 Kit | 183.6 EUR |
Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL |
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| SPXMT-KIT | Scientific Laboratory Supplies | 30ML | 237.12 EUR |
ProtoBlock System |
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| NAT1004 | Scientific Laboratory Supplies | 1KIT | 71.82 EUR |
New system |
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| VS10WPGS2 | Scientific Laboratory Supplies | EACH | 80.94 EUR |
PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
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| PIN320A-KIT | SBI | 1 Kit | 5929.2 EUR |
Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL |
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| SPXHM-KIT | Scientific Laboratory Supplies | 30ML | 245.1 EUR |
PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
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| PIN340iPS-KIT | SBI | 1 Kit | 5929.2 EUR |
AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
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| GE620A-KIT | SBI | 1 kit | 2558.4 EUR |
AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
|||
| GE622A-KIT | SBI | 1 kit | 2558.4 EUR |
AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
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| GE624A-KIT | SBI | 1 kit | 2558.4 EUR |
Genorise® Customized PCR primers for cloning and sequencing |
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| GR108023 | Genorise Scientific | 2 nmol | 78 EUR |
vWF Acty. Kit |
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| ABP-ACT-KIT | Abpcorp | 12 x 8 microwells | 513.6 EUR |
vWF Ant. Kit |
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| ABP-TOT-KIT | Abpcorp | 12 x 8 microwells | 472.8 EUR |
AEC coloring system |
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| CT356 | U-CyTech | 10-plate | 169.2 EUR |
data acquisition system |
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| D230 | Consort | ea | 2044.8 EUR |
CometAssay Electrophoresis System |
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| 4250-050-ES | Biotechne | 1 Kit | 3540.96 EUR |
Cometslides Rack System |
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| 4252-040-02 | Biotechne | 1 Each | 256.56 EUR |
CCD cooling system |
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| 3600100 | Atto | 1unit | 5896.8 EUR |
Fast Mutagenesis System |
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| 20-abx098073 | Abbexa |
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photo documentation system |
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| VLBPCX4-20M | Consort | ea | 7622.4 EUR |
photo documentation system |
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| VLBPCX4-20MX | Consort | ea | 8128.8 EUR |
photo documentation system |
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| VLDP-HOOD | Consort | ea | 6100.8 EUR |
photo documentation system |
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| VLDP17-26M | Consort | ea | 9751.2 EUR |
photo documentation system |
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| VLDP17-26MX | Consort | ea | 10156.8 EUR |
Disposable Bag System |
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| H20CBS50 | Scientific Laboratory Supplies | EACH | 165.3 EUR |
IsoTherm-System Starter |
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| E3880001018 | Scientific Laboratory Supplies | EACH | 163.02 EUR |
miniONE Electrophoresis System |
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| ELE3400 | Scientific Laboratory Supplies | EACH | 624.72 EUR |
Heidolph clamping system |
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| 509-63100-00 | Scientific Laboratory Supplies | EACH | 135.66 EUR |
Campact Vacuum System |
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| AC701 | Scientific Laboratory Supplies | EACH | 2531.94 EUR |
Extractor System Option |
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| DRY1128 | Scientific Laboratory Supplies | EACH | 476.52 EUR |
Lower cooling system |
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| WAT4796 | Scientific Laboratory Supplies | EACH | 984.96 EUR |
Propane Transfer System |
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| M-CEM-37015 | MiTeGen | 1 UNIT | 625 EUR |
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Description: Propane Transfer System |
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Digestive system (esophagus |
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| GI1441 | TissueArray | each | 306 EUR |
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Description: Digestive system (esophagus, stomach, colon, rectum, liver, pancreas) cancer with matched adjacent normal tissue array, including TNM, pathology grade and clinical stage, 72 cases/144 cores, replaced by GI1441a |
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Digestive system (esophagus |
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| GI701a | TissueArray | each | 222 EUR |
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Description: Digestive system (esophagus, stomach, colon, rectum, liver, pancreas) cancer with matched adjacent normal tissue array, including TNM, pathology grade and clinical stage, 35 cases/70 cores, replacing GI701 |
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PrecisionX Multiplex gRNA Cloning Kit |
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| CAS9-GRNA-KIT | SBI | 10 rxn | 534 EUR |
SBA Clonotyping System-AP Kit (OKSB00002) |
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| OKSB00002 | Aviva Systems Biology | 1 Kit | 1015.2 EUR |
|
Description: Description of target: ;Species reactivity: ;Application: ELISA, ELISPOT;Assay info: ;Sensitivity: |
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SBA Clonotyping System Kit : HRP (OKSB00003) |
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| OKSB00003 | Aviva Systems Biology | 1 Kit | 944.4 EUR |
|
Description: Description of target: ;Species reactivity: ;Application: ELISA;Assay info: ;Sensitivity: |
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UreaGel 29:1 System 1L Kit |
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| NAT1128 | Scientific Laboratory Supplies | EACH | 127.68 EUR |
hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9) |
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| CAS620A-KIT | SBI | 1 kit | 2582.4 EUR |
|
Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression) |
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mRNAExpress mRNA Synthesis kit (5 reactions) |
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| MR-KIT-1 | SBI | 5 reactions | 1382.4 EUR |
42 Component Terpene Kit in Methanol |
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| CAN-TERP-KIT | Scientific Laboratory Supplies | 1ML | 287.28 EUR |
Canadian Pesticide Kit Containing All 6 Mixes |
|||
| CAN-CAN-KIT | Scientific Laboratory Supplies | 1ML | 758.1 EUR |
AxioCam Erc5s rev 2, w/Accessory Kit |
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| ZV-Erc5sAC-Kit | MiTeGen | 1 KIT | 1611 EUR |
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Description: AxioCam Erc5s rev 2, w/Accessory Kit- connection cable Y-USB 2.0.- HDMI cable to DVI-D- SD card- IR remote control |
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cfMAX cfDNA Isolation System |
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| CFMAX100A-1 | SBI | 1 Kit | 574.8 EUR |
modular system 10x10 cm |
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| EVS1100-MULTI | Consort | ea | 1436.4 EUR |
modular system 20x10 cm |
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| EVS1200-MULTI | Consort | ea | 1741.2 EUR |
modular system 20x20 cm |
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| EVS1300-MULTI | Consort | ea | 2349.6 EUR |
vertical system 10x10 cm |
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| EVS3100-SYS | Consort | ea | 930 EUR |
vertical system 20x10 cm |
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| EVS3200-SYS | Consort | ea | 1741.2 EUR |
vertical system 20x20 cm |
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| EVS3300-SYS | Consort | ea | 2044.8 EUR |
mini system 7x8 cm |
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| EHS3100-SYS | Consort | ea | 524.4 EUR |
mini system 8x10 cm |
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| EHS1050-SYS | Consort | ea | 422.4 EUR |
FractionPREP Cell Fractionation system |
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| 55R-1359 | Fitzgerald | 50 assays | 909.6 EUR |
|
Description: Cell Fractionation system for use in the research laboratory |
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Fast MultiSite Mutagenesis System |
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| abx098861-10rxns | Abbexa | 10 rxns | 828 EUR |
myGel Mini Electrophoresis System |
|||
| BM0286 | GenDepot | System | 741.6 EUR |
EasyDip Slide Staining System |
|||
| M900-12AS-ASSORTED | ScyTek Laboratories | 1 Case(s) | 211.2 EUR |
BCIP/NBT Substrate System |
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| GR103022 | Genorise Scientific | 2 x 25 mL | 142.8 EUR |
AP Fluorescent Substrate System |
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| GR103053 | Genorise Scientific | Each | 99.6 EUR |
HRP Floresce Substrate System |
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| GR103054 | Genorise Scientific | Each | 99.6 EUR |
amfiRivert Reverse Transcription System |
|||
| R5200-050 | GenDepot | 50 rxns | 465.6 EUR |
Episomal iPSC Reprogramming System |
|||
| SC900A-1 | SBI | 5 reactions | 1161.6 EUR |
96-Well Vacuum System |
|||
| SD5011 | Bio Basic | 1 UNIT | 388.86 EUR |
Dual Spot PLUS System |
|||
| MIC2516 | Scientific Laboratory Supplies | EACH | 938.22 EUR |
KNF SC920G Vacuum System |
|||
| PUM5198 | Scientific Laboratory Supplies | EACH | 4664.88 EUR |
Axygen Gel Documentation System |
|||
| GD-1000 | Scientific Laboratory Supplies | EACH | 5947.38 EUR |
Syngene DigiGenius System DG1 |
|||
| ELE7500 | Scientific Laboratory Supplies | EACH | 2394 EUR |
Syngene InGenius 3 System |
|||
| ELE7540 | Scientific Laboratory Supplies | EACH | 4075.5 EUR |
Syngene GBox F3 System |
|||
| ELE7580 | Scientific Laboratory Supplies | EACH | 8413.2 EUR |
Foot Pedal Complete System |
|||
| FLY1234 | Scientific Laboratory Supplies | EACH | 706.8 EUR |
MiniVac Waste Aspiration System |
|||
| ARG1464 | Scientific Laboratory Supplies | EACH | 254.22 EUR |
Nalgene System 100 Cryobox |
|||
| CRY8305 | Scientific Laboratory Supplies | PK10 | 199.5 EUR |
Ez C02 System Sachets |
|||
| 260679 | Scientific Laboratory Supplies | PK20 | 126.54 EUR |
Ez Campy System Sachets |
|||
| 260680 | Scientific Laboratory Supplies | PK20 | 64.98 EUR |
Ez Anaerobe Pouch System |
|||
| 260683 | Scientific Laboratory Supplies | PK20 | 117.42 EUR |
VeriClean Triple Bucket System |
|||
| CLE9830 | Scientific Laboratory Supplies | EACH | 1629.06 EUR |
AFS-EDI SYSTEM CART |
|||
| ZAFSCART2 | Scientific Laboratory Supplies | EACH | 800.28 EUR |
Connector 5m System-POD |
|||
| ZFC0NN5SQ | Scientific Laboratory Supplies | EACH | 135.66 EUR |
Safevac Vacuum Aspiration System |
|||
| SLS4950 | Scientific Laboratory Supplies | EACH | 1567.5 EUR |
Automatic Potentiometric Titration System |
|||
| TIT1030 | Scientific Laboratory Supplies | EACH | 5647.56 EUR |
EpiSonic 2000 Sonication System |
|||
| EQC-2000-1 | EpiGentek | 1 unit | 20590.9 EUR |
myGel Mini Electrophoresis System |
|||
| E1101 | Benchmark Scientific | 1 each | 623.9 EUR |
Digestive system tissue array |
|||
| DGS2081 | TissueArray | each | 474 EUR |
|
Description: Digestive system tissue array, including TNM, stage and grade, 104 cases/208 cores |
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ExoAb Antibody Kit (CD9, CD63, CD81, Hsp70 antibodies, rabbit anti-human) with goat anti-rabbit HRP secondary antibody |
|||
| EXOAB-KIT-1 | SBI | 25 ul each | 752.4 EUR |
CLOuD9 Gene Expression Regulation Kit (includes 10 ug each of dCas9-PYL1 and dCas9-ABI1 lentivectors, and 100 ul of 0.5M Inducer Agent) |
|||
| CASCL9-100A-KIT | SBI | 1 Kit | 1358.4 EUR |
pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
|||
| CVD19-520A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-550A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-575A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE Alpha Combo Kit, includes Cat# CVD19-590A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-599A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE Gamma Combo Kit, includes Cat# CVD19-630A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-639A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE Beta Combo Kit, includes Cat# CVD19-640A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-649A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE Delta Combo Kit, includes Cat# CVD19-650A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-659A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE Omicron Combo Kit, includes Cat# CVD19-660A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-669A-KIT | SBI | 1 kit | 1162 EUR |
pPACK-SPIKE B.1.429 Combo Kit, includes Cat# CVD19-610A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-619A-KIT | SBI | 1 kit | 1141 EUR |
pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution (Cat# LV810A-1) |
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| CVD19-629A-KIT | SBI | 1 kit | 1141 EUR |
Human Visual system homeobox 1(VSX1) ELISA kit |
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| E01V0077-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Human Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Visual system homeobox 1(VSX1) ELISA kit |
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| E01V0077-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Human Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Visual system homeobox 1(VSX1) ELISA kit |
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| E01V0077-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
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Description: A competitive ELISA for quantitative measurement of Human Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Visual system homeobox 2(VSX2) ELISA kit |
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| E01V0078-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Human Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Visual system homeobox 2(VSX2) ELISA kit |
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| E01V0078-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Human Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Visual system homeobox 2(VSX2) ELISA kit |
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| E01V0078-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
|
Description: A competitive ELISA for quantitative measurement of Human Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Visual system homeobox 1(VSX1) ELISA kit |
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| E03V0077-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Mouse Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Visual system homeobox 1(VSX1) ELISA kit |
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| E03V0077-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Mouse Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Visual system homeobox 1(VSX1) ELISA kit |
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| E03V0077-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
|
Description: A competitive ELISA for quantitative measurement of Mouse Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Visual system homeobox 2(VSX2) ELISA kit |
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| E03V0078-192T | BlueGene | 192 tests | 1524 EUR |
|
Description: A competitive ELISA for quantitative measurement of Mouse Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Visual system homeobox 2(VSX2) ELISA kit |
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| E03V0078-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Mouse Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse Visual system homeobox 2(VSX2) ELISA kit |
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| E03V0078-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
|
Description: A competitive ELISA for quantitative measurement of Mouse Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Visual system homeobox 1(VSX1) ELISA kit |
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| E06V0077-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Goat Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Visual system homeobox 1(VSX1) ELISA kit |
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| E06V0077-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Goat Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Visual system homeobox 1(VSX1) ELISA kit |
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| E06V0077-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
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Description: A competitive ELISA for quantitative measurement of Goat Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Visual system homeobox 2(VSX2) ELISA kit |
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| E06V0078-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Goat Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Visual system homeobox 2(VSX2) ELISA kit |
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| E06V0078-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
|
Description: A competitive ELISA for quantitative measurement of Goat Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Visual system homeobox 2(VSX2) ELISA kit |
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| E06V0078-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
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Description: A competitive ELISA for quantitative measurement of Goat Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Visual system homeobox 1(VSX1) ELISA kit |
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| E08V0077-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Canine Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Visual system homeobox 1(VSX1) ELISA kit |
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| E08V0077-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Canine Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Visual system homeobox 1(VSX1) ELISA kit |
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| E08V0077-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
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Description: A competitive ELISA for quantitative measurement of Canine Visual system homeobox 1(VSX1) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Visual system homeobox 2(VSX2) ELISA kit |
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| E08V0078-192T | BlueGene | 192 tests | 1524 EUR |
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Description: A competitive ELISA for quantitative measurement of Canine Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Visual system homeobox 2(VSX2) ELISA kit |
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| E08V0078-48 | BlueGene | 1 plate of 48 wells | 624 EUR |
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Description: A competitive ELISA for quantitative measurement of Canine Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Visual system homeobox 2(VSX2) ELISA kit |
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| E08V0078-96 | BlueGene | 1 plate of 96 wells | 822 EUR |
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Description: A competitive ELISA for quantitative measurement of Canine Visual system homeobox 2(VSX2) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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At 24 h after transfection, the protein expression ranges of IGF-Ⅰ, sort Ⅰ collagen, and α-SMA had been detected by Western blotting. The quantity of samples in cell experiment was 3. Data had been statistically analyzed with evaluation of variance for factorial design, one-way evaluation of variance, impartial pattern t check, and chi-square check.