Esophageal cancer (EC) is one of essentially the most malignant and deadly digestive‑associated tumors worldwide. However, acquired drug resistance is a significant impediment regarding anticancer chemotherapy. An growing quantity of research have reported that microRNAs (miRNAs/miRs) are implicated in regulating the sensitivity of drug resistance in esophageal squamous cell carcinoma (ESCC).
The intention of the current examine was to examine the position of miR‑106b‑3p in the sensitivity of cisplatin for ESCC. Initially, reverse transcription‑quantitative polymerase chain response (RT‑qPCR) was carried out to analyze miR‑106b‑3p and protein‑glutamine γ‑glutamyltransferase E (TGM3) expression ranges in ESCC and non‑tumor adjoining tissues.
By utilizing bioinformatics software program TargetScan, TGM3 was predicted to be a possible downstream goal of miR‑106‑3p. Following verification that TGM3 was a downstream goal of miR‑106b‑3p by the twin‑luciferase reporter assay, the results of miR‑106b‑3p transfection on KYSE30 cell viability and apoptosis following therapy with cisplatin had been confirmed utilizing Cell Counting Kit‑eight and stream cytometry assays, respectively.
The outcomes revealed that miR‑106b‑3p ranges had been upregulated, whereas TMG3 ranges had been downregulated in ESCC tissues. Dual‑luciferase reporter assays confirmed that miR‑106b‑3p negatively regulated TGM3 expression by binding to its 3’UTR sequence. It was additionally proven that inhibition of miR‑106b‑3p might improve the anti‑proliferative results, whereas selling the apoptotic results of cisplatin in the KYSE30 cell line by targeting TGM3. In conclusion, the current examine demonstrated that downregulation of miR‑106b‑3p could improve the sensitivity of KYSE30 cell to cisplatin by targeting TGM3.
Ketogenic food plan alleviates colitis by discount of colonic group Three innate lymphoid cells by way of altering intestine microbiome
Accumulating proof means that ketogenic diets (KDs) mediate the rise of circulating ketone our bodies and exert a possible anti-inflammatory impact; nonetheless, the results of this distinctive food plan on colitis stay unknown. We carried out a sequence of systematic research utilizing a dextran sulfate sodium (DSS) animal mannequin of inflammatory colitis.
Animals had been fed with a KD, low-carbohydrate food plan (LCD), or regular food plan (ND). Germ-free mice had been utilized in validation experiments. Colon tissues had been analyzed by transcriptome sequencing, RT2 profiler PCR array, histopathology, and immunofluorescence. Serum samples had been analyzed by metabolic assay equipment.
Fecal samples had been analyzed by 16S rRNA gene sequencing, liquid chromatography-mass spectrometry and gasoline chromatography-mass spectrometry. We noticed that KD alleviated colitis by altering the intestine microbiota and metabolites in a way distinct from LCD. Quantitative food plan experiments confirmed the distinctive affect of KD relative to LCD with a reproducible improve in Akkermansia, whereas the other was noticed for Escherichia/Shigella.
After colitis induction, the KD protected intestinal barrier operate, and diminished the manufacturing of RORγt+CD3– group Three innate lymphoid cells (ILC3s) and associated inflammatory cytokines (IL-17α, IL-18, IL-22, Ccl4). Finally, fecal microbiota transplantation into germ-free mice revealed that the KD- mediated colitis inhibition and ILC3 regulation had been depending on the modification of intestine microbiota.
Taken collectively, our examine presents a world view of microbiome-metabolomics modifications that happen throughout KD colitis therapy, and identifies the regulation of intestine microbiome and ILC3s as novel targets involving in IBD dietary remedy.
Expression and effect of microRNA-627 in human hypertrophic scar
To examine the expression and impact of microRNA-627 (miR-627) in human hypertrophic scar. The experimental analysis technique was used. From October 2019 to January 2020, hypertrophic scar tissue from 6 sufferers with hypertrophic scar (2 males and four females, aged (34±11) years) and the remaining regular pores and skin tissue from 6 trauma sufferers (Three males and three females, aged (35±13) years) after flap transplantation had been collected.
The above-mentioned 12 sufferers had been admitted to the General Hospital of Northern Theater Command and met the inclusion standards. The mRNA expression of miR-627 was detected by real-time fluorescent quantitative reverse transcription polymerase chain response.
The third to fifth passages of fibroblasts (Fbs) had been remoted from hypertrophic scar tissue and cultured for subsequent experiments after identification. Fbs from hypertrophic scar had been divided into miR-627 adverse management group, miR-627 mimic group, and miR-627 inhibitor group. The corresponding sequences had been transfected respectively. At 0 (instantly), 12, 24, 36, and 48 h after transfection, the cell viability was detected by thiazolyl blue technique; at 24 h after transfection, the apoptosis was detected by stream cytometry; at 24 h after transfection, the protein expression ranges of insulin-like development issue Ⅰ (IGF-Ⅰ), sort Ⅰ collagen, and α easy muscle actin (α-SMA) had been detected by Western blotting.
Two batches of Fbs from hypertrophic scar had been used, one batch was divided into IGF-Ⅰ wild sort+miR-627 adverse management group and IGF-Ⅰ wild sort+miR-627 mimic group, and the opposite batch was divided into IGF-Ⅰ mutant+miR-627 adverse management group and IGF-Ⅰ mutant+miR-627 mimic group. The corresponding sequences had been transfected respectively.
At 48 h after transfection, the expressions of luciferase and renal luciferase had been detected by luciferase reporter gene detection equipment, and the ratio of the 2 was calculated to mirror the exercise of IGF-Ⅰ. Fbs from hypertrophic scar had been divided into miR-627 adverse management group, miR-627 mimic alone group, and miR-627 mimic+IGF-Ⅰ group, and had been transfected with the corresponding sequences respectively.
 SequaGel MD SSCP Kit |
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| NAT1170 | National Diagnostics | 1KIT | EUR 211 |
 SequaGel MD Heteroduplex Kit |
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| NAT1172 | National Diagnostics | EACH | EUR 221 |
 100ML SequaGel Buffer |
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| NAT1140 | National Diagnostics | 100ML | EUR 67 |
 450ML SequaGel XR |
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| NAT1160 | National Diagnostics | 450ML | EUR 105 |
 1L SequaGel XR |
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| NAT1162 | National Diagnostics | 1L | EUR 138 |
) PinPoint-HR System for Platform Cell Line Generation & Retargeting (includes PIN400A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN400A-KIT | SBI | 1 Kit | EUR 2798 |
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 Chymotrypsin for Sequencing grade |
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| C4001-010 | GenDepot | 4x25ug | EUR 313 |
Chymotrypsin for Sequencing grade |
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| C4001-100 | GenDepot | 100ug | EUR 286 |
 sequencing unit 33x45 cm |
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| ESEQ1100-SYS | Consort | ea | EUR 1535 |
 Trypin for Mass & Sequencing |
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| T9600-025 | GenDepot | 25ug | EUR 145 |
Trypin for Mass & Sequencing |
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| T9600-100 | GenDepot | 100ug | EUR 219 |
Trypin for Mass & Sequencing |
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| T9600-112 | GenDepot | 12x100ug | EUR 1657 |
Trypin for Mass & Sequencing |
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| T9600-400 | GenDepot | 4x100ug | EUR 651 |
) PinPoint-FC System for Platform Cell Line Generation & Retargeting (includes PIN300A-1, FC200PA-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN300A-KIT | SBI | 1 Kit | EUR 2798 |
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 450ML SequaGel UreaGel Concentrate |
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| NAT1132 | National Diagnostics | 450ML | EUR 105 |
 1L SequaGel UreaGel Concentrate |
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| NAT1134 | National Diagnostics | 1L | EUR 134 |
 200ML SequaGel UreaGel Buffer |
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| NAT1142 | National Diagnostics | 200ML | EUR 70 |
 450ML SequaGel UreaGel-6 |
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| NAT1144 | National Diagnostics | 450ML | EUR 96 |
 1L SequaGel UreaGel-6 |
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| NAT1146 | National Diagnostics | 1L | EUR 126 |
 450ML SequaGel UreaGel-8 |
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| NAT1148 | National Diagnostics | 450ML | EUR 96 |
 1L SequaGel UreaGel-8 |
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| NAT1150 | National Diagnostics | 1L | EUR 126 |
 450ML SequaGel UreaGel Diluent |
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| NAT1152 | National Diagnostics | 450ML | EUR 75 |
 1L SequaGel UreaGel Diluent |
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| NAT1154 | National Diagnostics | 1L | EUR 86 |
 90ML SequaGel Complete Buffer |
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| NAT1156 | National Diagnostics | 90ML | EUR 66 |
 100ML SequaGel XR Concentrate |
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| NAT1164 | National Diagnostics | 100ML | EUR 107 |
 450ML SequaGel XR Concentrate |
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| NAT1166 | National Diagnostics | 450ML | EUR 301 |
) PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN410A-KIT | SBI | 1 Kit | EUR 4335 |
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) PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1) |
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| PIN412A-KIT | SBI | 1 Kit | EUR 4335 |
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 DNA Library Prep Kit for IIlumina Sequencing |
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| K1475-12 | Biovision | 12 Rxns | EUR 480 |
 200ML SequaGel UreaGel Complete Buffer |
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| NAT1158 | National Diagnostics | 200ML | EUR 71 |
 200ML SequaGel MD Monomer Solution |
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| NAT1168 | National Diagnostics | 200ML | EUR 184 |
 PCR Clean Up for DNA Sequencing |
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| BT5100 | Bio Basic | 100preps | EUR 95.68 |
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PCR Clean Up for DNA Sequencing |
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| BT5101 | Bio Basic | 1000Preps, 1000prep | EUR 461.08 |
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 1.2ML SequaGel MD SSCP Stop Solution |
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| NAT1174 | National Diagnostics | 1.2ML | EUR 71 |
 Frit Kit |
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| FRIT-KIT | Next Advance | 1each | EUR 124 |
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Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool. |
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 Column Packing Kit |
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| PACK-KIT | Next Advance | 1pack | EUR 1035 |
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Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar. |
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 PCR Mycoplasma Detection Kit |
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| M034-Kit | TOKU-E | Kit | EUR 266 |
 Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit |
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| CAS400A-KIT | SBI | 1 kit (10 rxn) | EUR 1110 |
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 CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV100PA-KIT | SBI | 1 Kit | EUR 1132 |
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 CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV105PA-KIT | SBI | 1 Kit | EUR 1132 |
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 MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV120PA-KIT | SBI | 1 Kit | EUR 1132 |
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 MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV125PA-KIT | SBI | 1 Kit | EUR 1132 |
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 Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS700A-KIT | SBI | 10 rxn | EUR 1132 |
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 Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS720A-KIT | SBI | 10 rxn | EUR 1132 |
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 Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector |
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| CAS740A-KIT | SBI | 10 rxn | EUR 1132 |
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) T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents) |
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| CAS510A-KIT | SBI | 1 Kit | EUR 805 |
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-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV200PA-KIT | SBI | 1 Kit | EUR 1132 |
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-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV205PA-KIT | SBI | 1 Kit | EUR 1132 |
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-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV220PA-KIT | SBI | 1 Kit | EUR 1132 |
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-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit) Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit |
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| CASLV225PA-KIT | SBI | 1 Kit | EUR 1132 |
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 Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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| CAS750A-KIT | SBI | 10 rxn | EUR 1132 |
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 Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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| CAS770A-KIT | SBI | 10 rxn | EUR 1132 |
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 Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector |
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| CAS790A-KIT | SBI | 10 rxn | EUR 1132 |
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 and Fast ligase (2.5ul)]) Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)] |
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| CAS9LIG-KIT | SBI | 1 Kit | EUR 153 |
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 ProtoBlock System |
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| NAT1004 | National Diagnostics | 1KIT | EUR 102 |
) PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
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| PIN320A-KIT | SBI | 1 Kit | EUR 4941 |
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) PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1) |
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| PIN340iPS-KIT | SBI | 1 Kit | EUR 4941 |
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, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)) AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
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| GE620A-KIT | SBI | 1 kit | EUR 2132 |
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, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)) AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
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| GE622A-KIT | SBI | 1 kit | EUR 2132 |
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, Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)) AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site) |
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| GE624A-KIT | SBI | 1 kit | EUR 2132 |
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 CCD cooling system |
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| 3600100 | Atto | 1unit | EUR 4914 |
 CometAssay Electrophoresis System |
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| 4250-050-ES | Biotechne | 1 Kit | EUR 2950.8 |
 Cometslides Rack System |
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| 4252-040-02 | Biotechne | 1 Each | EUR 213.8 |
 data acquisition system |
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| D230 | Consort | ea | EUR 1704 |
 AEC coloring system |
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| CT356 | U-CyTech | 10-plate | EUR 141 |
 Fast Mutagenesis System |
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| 20-abx098073 | Abbexa |
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 Electrophoresis System Set |
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| E1101-E | GeneOn | 1 pcs | Ask for price |
 photo documentation system |
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| VLBPCX4-20M | Consort | ea | EUR 6352 |
photo documentation system |
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| VLBPCX4-20MX | Consort | ea | EUR 6774 |
photo documentation system |
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| VLDP-HOOD | Consort | ea | EUR 5084 |
photo documentation system |
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| VLDP17-26M | Consort | ea | EUR 8126 |
photo documentation system |
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| VLDP17-26MX | Consort | ea | EUR 8464 |
) hspCas9 AAVS1 Safe Harbor Knock-in Donor (AAVS1-SA-puro-EF1-hspCas9) |
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| CAS620A-KIT | SBI | 1 kit | EUR 2152 |
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Description: Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector), CAS640PR-1 (Junction PCR Primer Mix to confirm Cas9 integration site), and CAS9-PR-1 (PCR primers to confirm Cas9 expression) |
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 vWF Acty. Kit |
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| ABP-ACT-KIT | Abpcorp | 12 x 8 microwells | EUR 428 |
 vWF Ant. Kit |
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| ABP-TOT-KIT | Abpcorp | 12 x 8 microwells | EUR 394 |
 PrecisionX Multiplex gRNA Cloning Kit |
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| CAS9-GRNA-KIT | SBI | 10 rxn | EUR 445 |
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 UreaGel 29:1 System 1L Kit |
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| NAT1128 | National Diagnostics | EACH | EUR 143 |
) SBA Clonotyping System-AP Kit (OKSB00002) |
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| OKSB00002 | Aviva Systems Biology | 1 Kit | EUR 846 |
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Description: Description of target: ;Species reactivity: ;Application: ELISA, ELISPOT;Assay info: ;Sensitivity: |
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) SBA Clonotyping System Kit : HRP (OKSB00003) |
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| OKSB00003 | Aviva Systems Biology | 1 Kit | EUR 787 |
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Description: Description of target: ;Species reactivity: ;Application: ELISA;Assay info: ;Sensitivity: |
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) mRNAExpress mRNA Synthesis kit (5 reactions) |
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| MR-KIT-1 | SBI | 5 reactions | EUR 1152 |
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 FractionPREP Cell Fractionation system |
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| 55R-1359 | Fitzgerald | 50 assays | EUR 758 |
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Description: Cell Fractionation system for use in the research laboratory |
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 Fast MultiSite Mutagenesis System |
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| abx098861-10rxns | Abbexa | 10 rxns | EUR 690 |
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 mini system 8x10 cm |
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| EHS1050-SYS | Consort | ea | EUR 352 |
 mini system 7x8 cm |
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| EHS3100-SYS | Consort | ea | EUR 437 |
 cfMAX cfDNA Isolation System |
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| CFMAX100A-1 | SBI | 1 Kit | EUR 479 |
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 myGel Mini Electrophoresis System |
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| BM0286 | GenDepot | System | EUR 618 |
 modular system 10x10 cm |
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| EVS1100-MULTI | Consort | ea | EUR 1197 |
 modular system 20x10 cm |
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| EVS1200-MULTI | Consort | ea | EUR 1451 |
 modular system 20x20 cm |
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| EVS1300-MULTI | Consort | ea | EUR 1958 |
 vertical system 10x10 cm |
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| EVS3100-SYS | Consort | ea | EUR 775 |
 vertical system 20x10 cm |
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| EVS3200-SYS | Consort | ea | EUR 1451 |
 vertical system 20x20 cm |
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| EVS3300-SYS | Consort | ea | EUR 1704 |
 EasyDip Slide Staining System |
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| M900-12AS-ASSORTED | ScyTek Laboratories | 1 Case(s) | EUR 176 |
 96-Well Vacuum System |
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| SD5011 | Bio Basic | 1 UNIT | EUR 324.05 |
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 amfiRivert Reverse Transcription System |
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| R5200-050 | GenDepot | 50 rxns | EUR 388 |
 Episomal iPSC Reprogramming System |
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| SC900A-1 | SBI | 5 reactions | EUR 968 |
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At 24 h after transfection, the protein expression ranges of IGF-Ⅰ, sort Ⅰ collagen, and α-SMA had been detected by Western blotting. The quantity of samples in cell experiment was 3. Data had been statistically analyzed with evaluation of variance for factorial design, one-way evaluation of variance, impartial pattern t check, and chi-square check.